Quiz 4: Albumin Flashcards Preview

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Flashcards in Quiz 4: Albumin Deck (8)
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1

Pre-albumin (Transthyretin)

• Migrates ahead of albumin in electrophoresis
• Transport protein for thyroid hormones and Vitamin A (retinol).
• Synthesized in liver and is a major indicator of nutritional status of the patient.

2

Albumin Prevalence

• Synthesized in the Liver
• Albumin is the most abundant protein in the body.
• Most but not all urine protein is albumin

3

Albumin Measurement

• Measured by dye-binding methods (bromcresol green; bromcresol purple etc)
• 3.9 - 5.1 g/dL (almost 50% of the total protein in serum)

4

Functions of Albumin

• Control of colloid oncotic pressure (COP), the movement of water across membranes (high conc. of albumin maintains appropriate amount of fluid in tissues)
• Binding to substances in blood:
- carry hormones and drugs (bound vs. free)
- act as a buffer by binding free ions.

5

Increased Serum Albumin Levels

Found only in dehydration

6

Decreased Serum Albumin Levels

Found in a wide variety of conditions including:
(a) decreased synthesis in the liver
(b) hepatic disease (c) urinary loss
(d) malnutrition

7

A/G Ratio Calculation and Ranges

• A measure of the ration between the two major forms of protein found in the serum, albumin and the globulins.
• Total Protein = Albumin + Globulins
• Total Protein - Albumin = Globulins
• TP = 6.4 - 8.3 g/dL
• Albumin = 3.9 - 5.1 g/dL

8

Globulin Definition

• Second major category of proteins in serum.
• Consists of subgroups (based on their separation in electrophoresis) called:
alpha 1 and 2
beta 1 and 2
gamma fractions