Quiz 3: Cholesterol Testing Methods Flashcards Preview

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Flashcards in Quiz 3: Cholesterol Testing Methods Deck (6)
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Elevated Cholesterol

Atherosclerosis, coronary artery disease, increased risk of heart attack


Decreased Cholesterol

Liver Disease


Enzymatic Testing Methodologies

Cholesterol ester + water and cholesterol esterase gives cholesterol + fatty acid
Cholesterol + O2 and cholesterol oxidase gives cholestenone H2O2
H2O2 + phenol + 4-aminoantipyrine and peroxidase gives quinoneimine dye and water


HDL Cholesterol Testing

Increased levels are assoc. with decreased risk of coronary artery disease, decreased levels indicate the opposite
- Precipitate LDL/VLDL: with (dextran or heparin) sulfate-magnesium chloride, then assay the supernatant.
- Homogeneous assay: uses Ab to bind LDL and VLDL, enzymatic analysis can follow


LDL Cholesterol Testing

Assoc. with artherosclerosis and coronary heart disease
Calculated or measured directly
- Friedewald formula (indirect):
+ LDL = total cholesterol - [HDL + triglyceride/5]
- Homogeneous assay: uses detergents to keep HDL/VLDL from binding with dye, then an enzymatic assay is preformed


Triglyceride Testing Methodologies

Elevated levels assoc. with hyperlipoproteinemias, pancreatitis, alcoholism, obesity, hypothyroidism, nephrotic syndrome, and storage diseases
Triglyceride + water and lipase gives glycerol + fatty acids
Glycerol + ATP and glycerokinase gives glycerophosphate + ADP
Glycerophosphate + O2 and glycerophosphate oxidase gives dihydroxyacetone + H2O2
H2O2 + dye and peroxidase gives colored product and water