27. Creatinine Flashcards
What is Creatinine?
Chemical Waste Molecule
Generated from Muscle Metabolism
FROM Creatine
What are the Analytical Methods for Creatinine Investigation?
- Colorimetric Methods
- Express Reagent Strips
- Enzymatic Methods with UV Detection
- Methods with Ion-Selective Electrodes
1| What are the Colorimetric Methods for Creatinine?
Creatinine + Picric (In Alkaline Solution)
FORMING Orang-Yellow Compound = Chromogen
3| Which Method has a HIGH Specificity?
Enzymatic Methods
With UV Detection
What are the Errors in Creatinine Investigation?
+VE FALSE Readings
-VE FALSE READINGS
What CAUSES the POSITIVE (+ve) FALSE Readings in Creatinine Investigation?
- Pyruvate Acetoacetate
- Glucose
- Uric Acid
- a-Ketoacids
- Cephalosporines
What CAUSES the NEGATIVE (-ve) FALSE Readings in Creatinine Investigation?
INCREASED Levels of
- Bilirubin
- Hemoglobin
as they DESTROY the Chromogen
What are the Indications for Creatinine Investigation?
Damage of Nephrons
IN LATE Kidney Damage
What Specimen are Required for Creatinine Investigation?
Serum WITHOUT HEMOLYSIS
Urine
What is the Reference Range of Creatinine in MALE?
60 - 110 μmol / L
What is the Reference Range of Creatinine in FEMALE?
44 - 96 μmol / L
What Causes INCREASED Creatinine Levels?
DECREASED Glomerular Infiltration by MORE than 50%
Condition IMPAIRING Kidney Function
Drugs
Consumption of LARGE AMOUNTS of Dietary Meat
What Causes DECREASED Creatinine Levels?
LOWER Muscle Mass, FROM a Disease = Muscular Dystrophy; Aging
Severe Liver Diseases
VERY LOW Protein
Pregnancy
