Lab Practical 1 Flashcards
What 3 organisms are used in the disinfectant lab?
Bacillus cereus, Escherichia coli, and Staphylococcus epidermidis
What disinfectants are used in the disinfectant lab?
Vinegar, bleach, Purell, mouthwash, and hibiclens
What was the growth medium for the disinfectant lab?
Nutrient agar
How did we add the organisms for the disinfectant lab?
Pour plate
Where does Staphylococcus epidermidis reside?
On the skin
Where does Escherichia coli reside?
In the colon
What organisms were used in the osmosis lab?
Saccharomyces cervisiae, Bacillus cereus, Penicillium chrysogenum, Pseudomonas fluorescens, and Serratia marcescens
What was tested in the osmosis lab?
Salt and sugar
What was used as a control in the osmosis lab?
Nutrient agar
What is 1 method of food preservation?
Fridge/freezer or salt
What tool do you use to perform a streak?
An applicator stick
What are the purposes of heat fixing bacteria to a slide?
Kills the organisms and sticks the bacteria to the slide
What are simple stains used to study?
Cell structure/morphology
What organisms are used in the staining lab?
Staphylococcus epidermidis and Escherichia coli
Primary stain of a Gram stain?
Crystal violet
Counterstain of a Gram stain?
Safranin
Mordant of a Gram stain?
Iodine
Decolorizer for a Gram stain?
Alcohol
After staining, gram-positive bacteria is what color?
Violet
After staining, gram-negative bacteria is what color?
Pink
What kingdom does Penicillium belong to?
Fungi
Observe the Penicillium picture
Done
What is a common name for Penicillium?
Mold
What kingdom does Candida belong to?
Fungi
What does Penicillium look like?
A hand with fingers or broccoli
What is a common name for Candida?
Yeast
What kingdom does Giardia belong to?
Protista
Observe the Giardia pictures
Done
What does Giardia look like?
In the trophozoite phase, it looks like a tadpole with a smile
In the cyst stage, it looks like an egg with a smile
How many nuclei and flagella does Giardia have?
2 nuclei, 8 flagella
What kingdom does Trichomonas belong to?
Protista
Stages of Giardia?
Trophozoite and cyst
Stages of Trichomonas?
Only the trophozoite stage
Observe the Trichomonas picture
Done
What does Trichomonas look like?
Like Giardia with only 1 nucleus
What kingdom does Trypanosoma belong to?
Protista
List the fungi from the eukarya domain
Penicillium, Candida
List the protista from the eukarya domain
Trichomonas, Trypanosoma, Giardia
List the animal kingdom members from the eukarya domain
Clonorchis, Enterobius, Taenia saginata/solium, Xenopsylla, Ixodes, Pediculus humanus capitis/corporis or Pediculus Pthirus pubis, and Sarcoptes
What does Trypanosoma look like?
Like a tiny worm around RBCs
Observe the Trypanosoma image
Done
Notes about Trypanosoma?
Blood smear
Observe the Enterobius image
Done
What do the Enterobius look like?
Worms. Male is curved and shorter, female is longer and straighter
What is the common name for Enterobius?
Pin worm
What kingdom does Clonorchis belong to?
Animal
Phylum and class of Clonorchis?
Phylum - Platyhelminthes
Class - Trematoda
What is a common name for Clonorchis?
Liver fluke
What is notable about Clonorchis?
It is a hermaphrodite