AHG

Question 1

what is the antiglobilin test known as

Answer 1

Coombs test

Question 2

what immunoglobin has to be on RBC for agglutination to be visible in a Coombs test

Answer 2

IgG

Question 3

what animal does anti-human globulin come from

Answer 3

rabbits

Question 4

what does AHG bind to during a Coombs test

Answer 4

human immunoglobulins

Question 5

what kind of antiglobulin test measures in vivo sensitization of RBC

Answer 5

direct

Question 6

what kind of antiglobulin test measures in vitro sensitization of RBC

Answer 6

indirect

Question 7

what is an example of an indirect anti globulin test

Answer 7

ab screen test

Question 8

what kind of sample is used in a indirect Antiglobulin test

Answer 8

serum

Question 9

during a direct antiglobulin test what does the reagent detect

Answer 9

IgG or C’

Question 10

in a direct antiglobulin test what are the RBC washed with

Answer 10

saline

Question 11

what needs to be added if a neg Coombs test is received

Answer 11

checked cells

Question 12

what do checked cells verify

Answer 12

adequate washing, Anti-human globulin was added and working

Question 13

after checked cells are added and a pos test is recored what does this mean

Answer 13

test is valid

Question 14

after checked cells are added and a neg test is recored what does this mean

Answer 14

test is invalid and must be repeated

Question 15

if a pos direct antiglobulin test uses poly specific AHG what need to be done

Answer 15

repeat using monospecific AHG reagent

Question 16

what can cause the AHG reagent to become contaminated

Answer 16

putting vial in pt sample

Question 17

what is detected in an indirect antiglobulin test

Answer 17

irregular abs in recipient sera

Question 18

what is another name for Coombs reagent

Answer 18

antihuman globulin

Question 19

what color is poly specific AHG reagent

Answer 19

clear

Question 20

what does ploy specific AHG contain

Answer 20

abs to human IgG and human C3d

Question 21

what is an advantage of polyspecific AHG

Answer 21

detects C’ binding Abs on RBC

Question 22

what is an disadvantage of polyspecific AHG

Answer 22

detects C’ on cells that may not be clinically sig

Question 23

what is the color of IgG monospecific AHG

Answer 23

green

Question 24

what does IgG monospecific AHG contain

Answer 24

abs to human IgG only

Question 25

what color is C3d monospecific AHG

Answer 25

clear

Question 26

what size is C3d monospecific AHG

Answer 26

small

Question 27

what does C3d monospecific AHG contain

Answer 27

abs to human C3d only

Question 28

what is an advantage of monospecific AHG

Answer 28

fewer nuisance pos

Question 29

who regulates Coombs reagents

Answer 29

FDA

Question 30

what do CC reagent contain

Answer 30

regent RBC coated with IgG

Question 31

A description of the antiglobulin test is:

Answer 31

AHG reacts with human globulin molecules bound to RBCs

Question 32

Polyspecific AHG reagent contains:

Answer 32

Anti-IgG and anti-C3d.

Question 33

Monoclonal anti-C3d is Derived from

Answer 33

one clone of plasma cells.

Question 34

Which of the following is a clinically significant antibody whose detection has been reported in some instances to be dependent on anticomplement activity in polyspecific AHG?

Answer 34

anti-Jk a

Question 35

After the addition of IgG-coated RBCs (check cells) to a negative AHG reaction during an antibody screen, a negative result is observed. Which of the following is a correct interpretation based on these findings?

Answer 35

The antibody screen cannot be interpreted.

Question 36

RBCs must be washed in saline at least three times before the addition of AHG reagent to:

Answer 36

Remove traces of free serum globulins.

Question 37

An in vivo phenomenon associated with a positive is:

Answer 37

Maternal antibody coating fetal RBCs.

Question 38

False-positive DAT results are most often associated with:

Answer 38

Use of refrigerated, clotted blood samples in which complement components coat RBCs in vitro.

Question 39

Polyethylene glycol (PEG) enhances antigen-antibody reactions by:

Answer 39

Concentrating antibody by removing water.

Question 40

Solid-phase antibody screening is based on:

Answer 40

Adherence

Question 41

A positive DAT may be found in which of the following situations

Answer 41

HDFN

Question 42

What do Coombs’ check cells consist of?

Answer 42

Type O-positive cells coated with anti-D

Question 43

Which of the following IAT methods requires the use of check cells?

Answer 43

Manual tube method with albumin

Question 44

Which uncontrollable factor can affect AHG testing?

Answer 44

Incubation time

Question 45

Which would be the most efficient method for a laboratory staffed by medical laboratory technicians?

Answer 45

Polybrene

Question 46

A 27-year-old group O mother has just given birth to a group A baby. Since the mother has IgG anti-A, anti-B and anti-A, B in her plasma, which of the following methods and tests would be most effective at detecting the anti-A on the baby’s RBCs?

Answer 46

IAT using gel