Rh Blood Group Flashcards
(92 cards)
1
Q
what is the second most important blood group
A
Rh blood group
2
Q
does the Rh blood group have naturally occurring AB
A
no
3
Q
what kind of inheritance does the Rh blood group have
A
mendelian
4
Q
what kind of chromosomes is the RH gene on
A
haplotype
5
Q
T or F: the D ag is not immunogenic
A
F
6
Q
presence of D ag is considered RH ___
A
pos
7
Q
what chromosome are RHD and RHCE gene
A
chromosome 1
8
Q
what does RHD code for
A
presence/ absence of the D protein
9
Q
what does RHCE code for
A
presence of other proteins
10
Q
what chromosome is the RHAG gene located on
A
chromosome 6
11
Q
what gene must be present for successful expression of the Rh ag
A
RHAG
12
Q
what is the order of Rh ag that stimulate the strongest Ag response
A
D> c> E> C> e
13
Q
which Rh blood group system three separate closely linked genes that produce the ag
A
fisher-race
14
Q
which Rh blood group system? multiple alleles at one complex locus
A
wiener
15
Q
what kind of system does rosenfield use
A
numerical
16
Q
which Rh blood group system adapts well to computer entry
A
rosenfield
17
Q
what number is D ag (rosenfield)
A
1
18
Q
what number is C ag (rosenfield)
A
2
19
Q
what number is E ag (rosenfield)
A
3
20
Q
what number is c ag (rosenfield)
A
4
21
Q
what number is e ag (rosenfield)
A
5
22
Q
what Rh blood group system is used to talk to doctors
A
wiener
23
Q
what Rh blood group is on ISBT labels
A
rosenfield
24
Q
def? 6 digit number for each ag specificity
A
ISBT
25
what do the first 3 digits of the ISBT number indicate
blood group system
26
what do the last 3 digits of the ISBT number indicate
ag specificity
27
what are the 2 functions of the Rh ag
mmb integrity and intracellular cation transport
28
if someone has membrane abnormalities or RBC shortened survival what kind of Rh ag
Rh null
29
what race has the most Rh pos population
asians
30
what causes deleted phenotypes
replacement of large portions of RHCE gene with RHD gene
31
if the RBC lack C/c and or E/e what is the result
enhanced D activity
32
Rh mod =
weakened expression of Rh ags
33
Rh null=
no Rh ags expressed
34
what gene is mutated to have Rh mod
RHAG
35
what kind of Rh null type has a mutated RHAG gene
regulator type
36
what kind of Rh null type has a mutated RHCE gene and deleted RHD gene
amorphic type
37
what kind of Rh blood group has mild hemolytic anemia and reticulocytes
Rh null
38
what kind of blood can Rh null pt be transfused with
Rh null blood
39
what category of weak D has a reduced amount of D ags
quantitative
40
what category of weak D has C inherited in the trans location
position effect
41
what category of weak D has a missing part
Mosaic
42
if c and D are in double doses what does it cause C to do
decreases the expression of D
43
what is put into the tube for forward ABO typing
1 drop of regent antisera + 1 drop of pt cells
44
what is put into the tube for reverse ABO typing
1 drop of pt serum + 1 drop of red cell reagent
45
is Rh typing forward or reverse typing
foward
46
what occurs as a result of not added reagent or serum
false neg
47
what rh ag typing reagent has limited availability; expensive; and lengthy incubation time
saline reactive
48
can you use saline reactive reagent with a weak D
no
49
what rh ag typing reagent increase the likelihood of false pos
high protein
50
what has to be run with a high protein rh reagent
manufacturer's control
51
can you use high protein rh reagent with a weak D
yes
52
what rh ag typing reagent relaxes ab molecule by breaking disulfide bonds. ab span distance between RBC in low protein medium
chemically modified
53
can chemically modified rh reagent be used with weak d
yes
54
what rh ag typing reagent hybridize to increase ab production and ab strength
monoclonal
55
what is the most common Rh ag typing reagent used
monoclonal
56
if a pt is weak D pos what are they considered
Rh pos
57
can you give weak d blood to a d neg pt
no
58
what do expecting mothers need to be tested for
weak d
59
if a mother is Rh neg what do they need to be tested for
weak d
60
t or f: some hospitals may or may not test transfusion recipients for weak d
t
61
what is done during a du test
incubate tubes at 37 C for 15 mins--> spin --> read
62
what is done during a Coomb's test
wash tubes with saline 3x --> add Coomb's reagent --> spin --> read
63
what happens if the Rh control is pos during a du test
test is invalid
64
are IgG clinically. significant
yes
65
what temp do Rh ab react at
body temp
66
do Rh ab bind C'
no
67
The Rh system genes are:
RHD and RHCE
68
What Rh antigen is found in 85% of the Caucasian population and is always significant for transfusion purposes?
Big D
69
How are weaker-than-expected reactions with anti-D typing reagents categorized?
weak D
70
Cells carrying a weak D antigen require the use of what test to demonstrate its presence?
Indirect antiglobulin test
71
How are Rh antigens inherited?
codominate alleles
72
Biochemically speaking, what type of molecules are Rh antigens?
proteins
73
Rh antibodies react best at what temperature (°C)?
37
74
Rh antibodies are primarily of which immunoglobulin class?
IgG
75
Rh antibodies have been associated with which clinical condition?
Hemolytic disease of the fetus and newborn
76
What do Rh null cells lack?
Rh ags
77
what is the fisher-race nomenclature from the Weiner nomenclature: R1r
DCe/ce
78
what is the fisher-race nomenclature from the Weiner nomenclature: R2R0
DcE/Dce
79
what is the fisher-race nomenclature from the Weiner nomenclature: RzR1
DCE/DCe
80
what is the fisher-race nomenclature from the Weiner nomenclature: r'r
Ce/ce
81
what is the rosenfield nomenclature from the Weiner nomenclature: R1r
RH: 1, 2, -3, 4, 5
82
what is the rosenfield nomenclature from the Weiner nomenclature: R2R0
RH: 1, -2, 3, 4, 5
83
what is the rosenfield nomenclature from the Weiner nomenclature: RzR1
RH: 1, 2, 3, -4, 5
84
what is the rosenfield nomenclature from the Weiner nomenclature: r'r
RH: -1, 2, -3, 4, 5
85
what ag are present: R1r
D, C, c, e
86
what ag are present: R2R0
D, E, c, e
87
what ag are present: RzR1
D, C, E, e
88
what ag are present: r'r
C, c, e
89
Which Rh phenotype has the strongest expression of D?
D-
90
An individual has the following serologic reactions: D+C+E+c+e+f–. What is the most probable genotype?
R1R2
91
Which of the following is the most common haplotype in the African American population?
Dce
92
If a patient who is R 1 R 1 is transfused with RBCs that are R o r , which antibody is he most likely to produce?
anti- c
