Bacterial sexually transmitted infections I Flashcards Preview

Year 1 Microbiology > Bacterial sexually transmitted infections I > Flashcards

Flashcards in Bacterial sexually transmitted infections I Deck (60):
1

What chlamydia species is the most common cause of genital infection and conjunctivitis?

Chlamydia trachomatis

2

Does chlamydia have peptidoglycan in its cell wall?

No

3

What is the shape of chlamydia bacteria?

Cocci

4

What is the infectious particle in chlamydia?

Elementary body

5

What is the replicating / metabolically active particle in chlamydia?

Reticulate body

6

Chlamydia inclusions are made up primarily of elementary bodies or reticulate bodies?

Reticulate bodies

7

The multiple chlamydia trachomatis serovars are based on what protein?

Major outer membrane protein

8

Chlamydia has a tropism for what type of cell?

Nonciliated, columnar, cuboidal, and transitional epithelial cells

9

How does chlamydia do cytotoxic damage?

Destruction of epithelium (cell lysis) and proinflammatory cytokine response

10

Without treatment, chlamydia can lead to what condition?

Fibrosis (infertility)

11

Which serovars are responsible for trachoma?

A, B, Ba, C

12

Which serovars are responsible for urogenital tract infection?

D-K

13

Which serovars are responsible for lymphogranuloma venerum?

L1, L2, L2a, L2b, L3

14

Why are LGV serovars more invasive than the other serovars?

Replication in mononuclear phagocytes

15

What is the leading cause of preventable blindness in the world?

Chronic chlamydia conjunctivitis

16

What are the symptoms of acute conjunctivitis in sexually active adults?

1. Mucopurulent discharge2. Keratitis 3. Corneal infiltrates 4. Occasional corneal vascularization

17

What can help prevent transmission of neonatal conjunctivitis?

Erythromycin eye drops

18

What is the presentation for male C. trachomatis UG infection?

Dysuria and thin urethral MUCOPURULENT discharge

19

What are the complications of male C. trachomatis UG infection?

Epididymitis, prostatitis

20

What is Reiter syndrome?

1. Complication of male C. trachomatis UG infection 2. Urethritis, conjunctivitis, polyarthritis

21

What is the presentation for female C. trachomatis UG infection?

Mucopurulent discharge

22

Is UG chlamydia infection more common in men or women?

Women

23

What disease presents with primary (painless) lesions / papules and inflammation and swelling of lymph nodes draining the site of infection with inguinal lymphadenopathy?

Lymphogranuloma venereum (LGV)

24

What is a common result of lymphatic spread in LGV?

Proctitis

25

What are the tests for C. trachomatis?

1. Giemsa stain 2. Iodine stain 3. Immunofluorscence 4. PCR

26

What does the Giemsa stain detect in C. trachomatis infection?

Cytoplasmic inclusions (need epithelial cells)

27

What does the iodone stain detect in C. trachomatis infection?

Reticulate bodies (need epithelial cells)

28

What does immunofluorescence detect in C. trachomatis infection?

Elementary bodies (need epithelial cells)

29

What sample is needed for PCR diagnosis of C. trachomatis?

Urine or urethral discharge

30

What is the treatment for C. trachomatis infection?

Doxycycline or macrolides

31

Does chlamydia infection confer immunity?

No

32

Is neisseria gram negative or gram positive?

Gram negative

33

What is the shape of neisseria?

Diplococci

34

Is neisseria aerobic or anaerobic?

Aerobic

35

Is neisseria catalase positive or negative?

Positive

36

Is neisseria oxidase positive or negative?

Positive

37

Does neisseria form spores?

No

38

How can N. meningitidis and N. gonorrhoeae be distinguished?

N. meningitidis ferments maltose, N. gonorrhoeae does not

39

What are the N. gonorrhoeae virulence factors?

1. Pilin 2. Por protein 3. Opa protein 4. LOS 5. Outer membrane blebs 6. IgA1 protease 7. B-lactamase

40

What is the role of pilin in N. gonorrhoeae?

Attachment, anti-phagocytic

41

What is the role of por protein in N. gonorrhoeae?

Promotes intracellular survival

42

What is the role of opa protein in N. gonorrhoeae?

Attachment to eukaryotic cells

43

What is the role of LOS in N. gonorrhoeae?

Endotoxin

44

What is the role of the outer membrane blebs in N. gonorrhoeae?

Contain LOS and OM proteins, enhance toxicity and absorb antibodies

45

What is the role of IgA1 protease?

Destroys IgA

46

What is the role of B-lactamase?

Hydrolyzes B-lactam ring in penicillin

47

Which N. gonorrhoeae virulence factors undergo antigenic variation?

1. Pilin 2. Por protein 3. Opa protein 4. LOS

48

Neisseria infections are associated with deficiencies in what complement components?

Late complement components C5b-C9

49

What are the late complement components responsible for?

Formation of the membrane attack complex (MAC) - lysis of pathogen

50

What is the horizontal spread of N. gonorrhoeae?

Eye - mouth - genitals

51

Does N. gonorrhoeae have a capsule?

No

52

How do the gonococci attach to mucosal cells?

Pili, PorB, Opa

53

What do gonococci do after invasion of mucosa?

1. Replicate inside cells, lysis 2. Phagocytosis by macrophage / neutrophil 3. LOS stimulates inflammation

54

What kind of discharge accompanies N. gonorrhoeae infection?

Mucopurulent discharge

55

What are the disseminated effects of N. gonorrhoeae infection?

Septicemia, skin and joint infections, suppurative arthritis, pustular rash on extremities

56

What always accompanies N. gonorrhoeae genital infection?

Pharyngitis

57

What is the diagnosis for N. gonorrhoeae infection?

1. Direct smear 2. Culture 3. PCR

58

What would a gram stain for N. gonorrhoeae reveal?

Gram negative bean shaped diplococci in NEUTROPHILS

59

How can N. gonorrhoeae be cultured?

1. Chocolate agar (nonselective) 2. Thayer-Martin media (selective)

60

What is the treatment for N. gonorrhoeae?

Ceftriaxone plus doxyclycline or azithromycin (for chlamydia since they probably have that too)