Viral infections of blood and tissues I Flashcards Preview

Year 1 Microbiology > Viral infections of blood and tissues I > Flashcards

Flashcards in Viral infections of blood and tissues I Deck (29):
1

Does EBV have an envelope?

Yes

2

What is the EBV genome?

dsDNA

3

How does EBV gain attachment and entry?

C3d

4

Where does EBV replicate?

Epithelial cells and B cells

5

What is the virology of EBV?

1. Infected B cells induced to proliferate 2. Heterophile Abs produced 3. T cells clear infection but some B cells remain (memory)

6

What are the genes involved in EBV carciogenesis?

Latent membrane protein 1 (LMP1), latent membrane protein 2 (LMP2), Epstein Barr viral nuclear antigen 1 (EBNA1)

7

What is the function of LMP1 in EBV?

Increased growth and suppressed apoptosis

8

What is the function of LMP2 in EBV?

Increased growth of B cells

9

What is the function of EBNA1 in EBV?

Controls expression of LMP1 and LMP2

10

When is a rash seen in infectious mononucleosis?

Following ampicillin treatment

11

What is the course of appearance of antibodies in infectious mononucleosis?

1. Anti-EA / Anti-VCA 2. IgM 3. Anti-EBNA / IgG

12

What is the diagnosis for infectious mononucleosis?

1. Mono spot test - heterophile Abs 2. Antibodies to EBV - IgM to viral capsule antigen (VCA) demonstrates primary infection 3. Downey cells

13

What are Downey cells?

Atypical T cell with vacuoles, altered nucleus, and indented cell margins

14

What are cofactors associated with Burkitt's lymphoma?

Chronic malaria and immune suppression

15

What are the clinical symptoms of Hodgkin's lymphoma?

Enlarged mediastinal lymph nodes, fever, night sweats, weight loss

16

What is the key cytological finding in Hodkin's lymphoma?

Reed Sternberg cell - large cell with two or more nuclei or nuclear lobes - each of which contains an eosinophilic nucleus

17

What are the symptoms of nasopharyngeal carcinoma?

Facial pain, fullness in sinuses and throat, hearing loss

18

What is the treatment for post-transplantation lymphoproliferative disorder?

1. Reduce immunosuppression 2. Treat with rituximab 3. Conventional chemotherapy

19

Is CMV enveloped?

Yes

20

What is the CMV genome?

dsDNA

21

Where does latency occur in CMV infection?

Monocyte

22

What are the primary antiviral therapies used for CMV?

Gancyclovir (IV / oral), valganciclovir (oral)

23

What is the method of action of gancyclovir?

Converted to polymerase inhibitor by CMV enzymes

24

What is the method of action of valganciclovir?

Converted to gancyclovir within the body

25

What drugs are used for resistant CMV?

Cidofovir, Foscarnet

26

How does CMV infectious mononucleosis-like illness differ from actual mononucleosis?

No heterophile antibodies, seen in infections of non-immunocompromised adults

27

What are the main symptoms of cytomegalic inclusion body disease?

Hepatosplenomegaly, jaundice, petechiae / rash

28

What is the risk of transmission from primary CMV infection during pregnancy?

33%

29

CMV retinitis is usually seen in what patient population?

AIDS patients