biochem 2 krebs cycle Flashcards Preview

Anatomy > biochem 2 krebs cycle > Flashcards

Flashcards in biochem 2 krebs cycle Deck (25):
1

What enzyme catalyses pyruvate to acetyl CoA?

PDC-----pyruvate dehydrogenase

2

what is the first subsrate in krebs cycle?

Acetyl CoA

3

What is the first reaction of kreb's cycle?

condensation of Acetyl CoA by enzyme citrate synthase to create citrate by the addition of water and the byproduct CoASH

4

What is the second reaction of Kreb's cycle?

the movement of hydroxyl group on Carbon 2 of citrate to carbon 3 of Isocitrate catalyzed by aconitase

5

What is the third reaction of Kreb's cycle?

the oxidation and decarboxylation of isocitrate to give
1.NADH + (H+) & CO2
2. Alpha-ketoglutarate

6

What is the catalyst of the third reaction?

isocitrate dehydrogenase

7

What is the fourth reaction of Kreb's cycle?

The oxidation and decarboxylation of alpha-ketogluarate to produce
1. NADH + (H+) & CO2
with the addition of CoASH
2.Succinyl CoA

8

What is the catalyst of the fourth reaction?

alpha-ketoglutarate dehydrogenase

9

What forms at the ketone end of Succinyl CoA after the fourth reaction?

a high energy bond

10

What is the fifth reaction of Kreb's cycle?

substrate level phosphorylation of Succinyl CoA (no O2 needed) to produce
1. CoASH
with the addition of GDP + P
2. GTP
3. Succinate

11

What is the catalyst used for the fifth reaction?

succinate thiokinase

12

What is the sixth reaction of Kreb's cycle?

oxidation of Succinate to produce (after the addition of FAD
1. FADH (2H)
2.Fumarate

13

What is the sixth reaction's catalyst?

succinate dehydrogenase

14

What is the seventh reaction of Kreb's cycle?

hydration of fumarate to produce (after the addition of H2O) hydration duh!
1. Malate by the calalyst fumarase

15

What is the eighth and final reaction of Kreb's cycle?

oxidation of Malate to produce (after the addition of NAD+
1. NADH + (H+)
2. Oxaloacetate

16

What is the catalyst of the eighth reaction?

malate dehydrogenase

17

What are the reaction steps that produce NADH + H+ or FADH (2H)

reaction
3. NADH + (H+)
4. NADH +(H+)
6. FADH (2H)
8. NADH +(H+)

18

What reaction (s) produce GTP?

reaction 5

19

What reactions produce CO2?

reaction 3 & 4

20

increased lactate produces _____

acidosis

21

Where is the 3 NADH and 1 FADH being sent?

ETC electron transport chain for oxidative phosphorylation

22

What are the first 4 substates used in Kreb's cycle?

1.Acetyl CoA
2.Citrate
3.Isocitrate
4.alpha-ketoglutarate

23

What are the first four catalysts used in Kreb's cycle?

1. citrate synthase
2.aconitase
3.isocitrate dehydrogenase
4.alpha-ketoglutarate dehydrogenase

24

What are the last 5 substrates used in Kreb's cycle?

5. Succinyl CoA
6. Succinate
7. Fumarate
8. Malate
9. Oxaloacetate

25

What are the last 4. catalysts used in Kreb's cycle?

5.succinate thiokinase
6.succinate dehydrogenase
7. fumarase
8. malate dehydrogenase