BIOLOGY- biology and microscopy Flashcards
(15 cards)
early microscopes (Hans and Zacharias Jansen)
-invented microscope in 1500’s
- ocular lens (eyepiece) and objective lens
- magnifying power of 20x
Robert Hooke
- 3 lens microscope
- uses beam of light
micrographia
illustrations of various living and non-living things as they appear under a microscope
what was robert hooke interested in?
the structure of cork, which was lightweight, could float, firm and compressible
who named cells
robert hooke
Anton Van Leeuwenhook
-single lens microscope
- could magnify 250x
who was the first to see movement of single celled organisms (animalcules)
anton van leeuwenhoek
parts of the microscope (draw it if you need)
- ocular lens
- body tube
- revolving nosepiece
- arm
- objectives
- stage
- stage clips
- diaphragm
- course adjustment knob
- fine adjustment knob (the smaller one)
- light source
- base
calculating total magnification
multiply magnifications of individual lenses (eg. 10x multiply 4x = 40x magnification)
object size formula
object size = filed of view diameter/ number of objects to fit across field of view
scale
drawing size/ object size
is scale recorded in ratios?
yes
why do we need to calculate the high power field of view?
because it can be too small to see
calculating high power FOV
low power FOV x low power magnification/ high power magnification
micrometers
1/1000th of a millimeter