Biosynthetic secretory pathway Flashcards
(28 cards)
Multiple internal protein sequences form a specific 3D conformation
Signal patch
_____ directs the ER signal sequence to a specific receptor in the rough ER membrane
SRP
SRP binds to
Large ribosomal subunit
Protein translocator
Sec61 complex
How does Sec61 complex open
- Plug moves out of the way
- Opens sideways at the seam
Translocator opens by binding the
Signal sequence
What happens to protein once it has completely passed through translocator
Enters the biosynthetic secretory pathway
What determines the cellular compartment the protein is targeted to
Signal sequence
Enzyme that cleaves the ER signal sequence
Signal peptidase
Where does folding and assembly into multimeric proteins occur
ER lumen
Most proteins synthesised in the rough ER are
Glycosylated
Glycosylation refers to the addition of
Oligosaccharides
The GPI anchor marks proteins for
Membrane insertion
True or false: proteins that are not properly folded and assembled can leave the ER
False
Selective gates that allow transport between the nucleus and cytoplasm
Gated transport (nuclear pores)
Protein transporters that require proteins to be unfolded to cross from the cytoplasm to topologically distinct areas
Transmembrane transport
Membrane enclosed transport intermediates move proteins between compartments
Vesicular transport
What direction do proteins with COPI coat go
Retrieval transport
What direction do proteins with COPII coat go
From the ER to the golgi
How is the direction of transport determined in vesicular transport
Coating on the vesicle
Three regions of the golgi apparatus
Cis, medial, trans
What organelle finishes the processing of oligosaccharides
Golgi apparatus
What components make up the golgi stack
Cis cisterna, medial cisterna, trans cisterna
Static golgi transport model
Vesicular
