Cell culture Flashcards
(15 cards)
Primary cell cultures
Derived from normal animal tissue
True or false: Primary cultures will grow indefinitely
False
_______________ cultures most accurately reflect the
cells in the tissue from which they came, but their
limited lifespan precludes many experiments
Primary cell
______________ are capable of indefinite growth in culture when given appropriate environment /nutrients
Continuous or transformed cell lines
_______________ less accurately reflect the cells in the tissues
from which they were derived, but are easy to propagate and
more amenable to experimental manipulation
Continuous cell lines
Can be differentiated into all
different cell types using specific
hormones and growth factors in
culture media
Embryonic stem cells
Adult cells derived from skin biopsies or blood cells that have been reprogrammed back into an embryonic-like pluripotent state
Adult Induced Pluripotent Stem Cells (iPSCs)
__________________ cells require a rich media and specific
growth conditions in a dedicated cell culture incubator
Mammalian
Minimal essential components in MEM
Glucose, salts, amino acids, vitamins, serum, may add phenol red
What are non adherent cell lines derived from
Specific cell lines derived from blood
Why do we dilute non-adherent cells
Gives the cells more space and access to more nutrients
What chemical is used in the passaging of adherent cells to break down focal adhesions
Trypsin
What happens in a confluent cell culture
Cells stop dividing G0, cell contact inhibition prevents further proliferation
What are hydrogels
Hydrated polymer networks
True or false: 3D culture models better mimic the cell environment in tissue
