Chaperoning protein folding Flashcards
(20 cards)
Labyrinth of tubules and flattened sacs, central role in lipid and protein biosynthesis
Endoplasmic reticulum (ER)
What is the largest membrane in a eukaryotic cell
Endoplasmic reticulum
What ER has membrane bound ribosomes
Rough ER
ER that handles synthesis, folding and post-translation modification of proteins
Rough ER
ER with no bound ribosomes
Smooth ER
ER that handles lipid synthesis, detoxification reactions and storage of calcium
Smooth ER
The smooth ER is involved in the transport of proteins from the _____ to the _________
ER, golgi
Molecules that speed up protein folding
Molecular chaperones
True or false: chaperones are only located in certain cellular compartments
False
How do molecular chaperones prevent aggregation
Recognise hydrophobic patches on the surface of proteins
Forms a new environment to surround misfolded protein and facilitate folding
Chaperonins
Chaperonins are a _________ complex
hsp60-like protein
What cap is added to the chaperonin
GroES cap
What machinery does a molecular chaperone have
hsp70 machinery
What marks how long peptide has been in the ER
Number of glucoses on precursor oligosaccharide
Retain protein in ER and continue cycle until it’s correctly folded
Lectins
Improperly folded proteins are exported from ER to _________ to be degraded (________)
cytoplasm, retrotranslocation
Three sensors for misfolded proteins in the unfolded protein response
IRE1, PERK, ATF6
Destroys proteins that don’t ever fold correctly
Proteolytic machinery
Hydrophobic regions on proteins interacting with each other
Aggregation
