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Hematology Module 2 > Cytochemistry > Flashcards

Flashcards in Cytochemistry Deck (15)
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staining constituents of cells to help determine lineage; used when cells are so immature that you cannot identify them from morphology alone

cytochemistry

1

enzyme found in primary granules of neutrophils; positive in myeloid and negative in lymphoid cells

Myeloperoxidase Stain (MPO)

2

stains lipids; positive in primary and secondary granules of neutrophils and the granules of monocytes; negative in lymphoid cells; slightly less sensitive than MPO

Sudan Black Stain (SBB)

3

primarily used to identify early granulocytic cells where red = positive; SPECIFIC ESTERASE

Naphthol-AS-D-Chloroacetate Esterase (NASDA)

4

primarily used to identify monocytic cells; NONSPECIFIC ESTERASE

Alpha Naphthyl Butyrate/Acetate Esterase- ANBE/ANAE

5

______ is positive in megakaryocytes while ______ is not

ANAE; ANBE

6

in ANBE red is positive in ____________ cells and the _______ cells are negative.

monocytic; myeloid

7

stains glycogen

Periodic Acid Schiff (PAS)

8

block positivity of PAS is present in what?

blasts of ALL

9

PAS is negative in normal RBC precursors , but positive in blasts of what?

erythroid type of AML

10

immunoperoxidase reaction for factor VIII is positive in what?

megakaryoblastic leukemia

11

RECAP
-MPO and SBB are positive for what cells?

Myeloid

12

RECAP
-NASDA is specific and stains for what?

granulocytes

13

RECAP
-ANBE is non-specific and is postive in what?

monocytic

14

RECAP
-PAS is positive in what?

in ALL and Leukemic or malignant RBCs