Flashcards in Cytochemistry Deck (15)
staining constituents of cells to help determine lineage; used when cells are so immature that you cannot identify them from morphology alone
enzyme found in primary granules of neutrophils; positive in myeloid and negative in lymphoid cells
Myeloperoxidase Stain (MPO)
stains lipids; positive in primary and secondary granules of neutrophils and the granules of monocytes; negative in lymphoid cells; slightly less sensitive than MPO
Sudan Black Stain (SBB)
primarily used to identify early granulocytic cells where red = positive; SPECIFIC ESTERASE
Naphthol-AS-D-Chloroacetate Esterase (NASDA)
primarily used to identify monocytic cells; NONSPECIFIC ESTERASE
Alpha Naphthyl Butyrate/Acetate Esterase- ANBE/ANAE
______ is positive in megakaryocytes while ______ is not
in ANBE red is positive in ____________ cells and the _______ cells are negative.
Periodic Acid Schiff (PAS)
block positivity of PAS is present in what?
blasts of ALL
PAS is negative in normal RBC precursors , but positive in blasts of what?
erythroid type of AML
immunoperoxidase reaction for factor VIII is positive in what?
-MPO and SBB are positive for what cells?
-NASDA is specific and stains for what?
-ANBE is non-specific and is postive in what?