Into to Flow Cytometry Flashcards
(60 cards)
1
Q
CD8
A
T-cell marker
2
Q
CD117
A
Immature marker
3
Q
CD 71
A
Erythroid precursor marker
4
Q
CD 19
A
B-cell marker
5
Q
CD4
A
T-cell marker
6
Q
Kappa light chain
A
B-cell marker
7
Q
TdT
A
Immature marker
8
Q
CD 41
A
Megakaryocyts marker
9
Q
CD 13
A
Granulocytic/monocytic marker
10
Q
CD3
A
T-cell marker
11
Q
Glycophorin A
A
Erythroid precursor marker
12
Q
Lambda light chain
A
B-cell marker
13
Q
CD 34
A
Immature AND granulocytic/monocytic marker
14
Q
CD 42
A
Megakaryocyte marker
15
Q
CD 14
A
Granulocytic/monocytic marker
16
Q
CD61
A
Megakaryocyte marker
17
Q
CD 33
A
Granulocytic/monocytic marker
18
Q
CD 20
A
B-cell marker
19
Q
CD5
A
T-cell marker
20
Q
CD 15
A
Granulocytic/monocytic marker
21
Q
CD7
A
T-cell marker
22
Q
CLL important cell morphology
A
Smudge cells
23
Q
Mantle Cell Lymphoma important Immunophenotype
A
Cyclin D1+
24
Q
Mantle Cell Lymphoma genetics
A
t(11;14)
25
Follicular Lymphoma important Immunophenotype
bc12+
26
Follicular Lymphoma genetics
t(14;18)
27
Hairy Cell Leukemia important cell morphology
Hairy Cells
28
Hairy Cell Leukemia important immunophenotype
CD11c, CD103+, CD123+, bright CD20+
29
Hairy Cell Leukemia genetics
BRAF-V600E
30
Hairy Cell Leukemia important additional feature
Trap stain positive
31
Burkitt Lymphoma important architecture
Diffuse with star sky pattern
32
Burkitt Lymphoma important cell morphology
Many vacuoles
33
Burkitt Lymphoma important immunophenotype
Ki67
34
Burkitt Lymphoma genetics
t(8;14)
35
Burkitt Lymphoma important additional features
Mainly in children and young adults
36
Cutaneous T-Cell Lymphoma/Mycosis fungoides important architecture
diffuse infiltrates into skin
37
Cutaneous T-Cell Lymphoma/Mycosis fungoides important cell morphology
sezary cells
38
Cutaneous T-Cell Lymphoma/Mycosis fungoides important immunophenotype
CD7- CD8-
39
Large Granular Lymphocyte Leukemia important immunophenotype
cd4-, cd8+,cd57+, cd16+, TIA1+, Gramzyme B+
40
Large Granular Lymphocyte Leukemia important genetics
STAT3 gene mutation
41
Classical Hodgkin Lymphoma important cell morphology
```
Reed-Sternberg (binucleated) cells
Hodgkin Cells (mononuclear)
```
42
Classical Hodgkin Lymphoma important immunophenotype
CD45-
43
Classical Hodgkin Lymphoma important additional features
present with localized lymph node involvement
44
Plasma Cell Myeloma important architecture
Plasma cell infiltrates in BM >30% of cellularity
45
Plasma Cell Myeloma important additional features
Monoclonal IgG or IgA in serum or urine; rouleaux; bone lesions
46
Morphologic and functional changes are caused by what?
genetic and epigenetic changes
47
Prominent cell type found in follicles
B-cells
48
Prominent cell type found in paracortex
T-Cell
49
Prominent cell type found in medulla
Plasma cells
50
CLL presents where and SLL presents where?
```
CLL = bone marrow
SLL = Lymph Nodes
```
51
Hodgkin Lymphoma is _____ in origin
B Cell
52
What are the important occurrences in Hairy Cell Leukemia
Pamcytopenia
splenomegaly
"fried egg appearance"
53
Age of onset for Burkitt Lymphoma
children and young adults
54
Age of onset for Hodgkin Lymphoma
median age is 30
55
lineage of plasma cells
B Cells
56
Flame Cells
- morphology
- antibody production
- plasma cell
| - producing IgA
57
Mott Cells
- morphology
- antibody production
- globules of immunoglobulin that stain pink, colorless or blue, inclusions called Russell Body
- produces IgG
58
Plasma cell myeloma produces what antibody(s)
Usually IgG, but uncommonly IgA
59
Waldenstrom's Macroglobulinemia produces what antibody(s)
IgM
60
cryoglobulinemic purpura is associated with what condition?
Waldenstrom's macroglobulinemia
