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Biology I (116) > DNA Extraction Lab > Flashcards

Flashcards in DNA Extraction Lab Deck (9)
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1

3 basic steps in DNA Extraction

1. cell must be lysed( broken open) to release the nucleus

2. Nucleus is open to release DNA

3. Once the DNA is released it must be precipitated out of the solution

2

reagents

a substance or mixture for use to produce a chemical reaction

3

reagents required

1. salt-
2. detergent-breaks down lipid membrane-
3. alcohol--ethanol precipitates the dna

4

both cell and nuclear membrane are made mostly of what macromolecule

lipids (phospholipid)

5

In order for the cell to be lysed what must first occur

The lipid membrane must first be broken down

6

What reagents are used to break down lipid membranes of cells (. How?

1. Manual grinding
2. detergent solution

Soap molecules mix with fats or lipids, causing structures made if lipids to break apart

7

alcohol--ethanol

precipitates the dna

8

Salt

It provides the Dna with a favorable environment by contributing POSITIVELY CHARGE atoms that neutralizes the normal negative charge of DNA, allowing it to clump together

9

the charge of DNA

NEGATIVE ( also pole/soluble)