Handout 4 Flashcards Preview

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Flashcards in Handout 4 Deck (20):
1

- formalin (37% in water) - denatures proteins and nucleic acids – alkylating agent

formaledhyde

2

ethylene oxide – for heat sensitive materials

GAS STERILIZATION

3

1) 2% gluteraldehyde – alkylated DNA and proteins

chemical sterilization
put in the chemiclve with formaldehyde and a little water

4

Dry heat 320F for 2 hours or 340F for 1 hour. Coagulates proteins – safe for metal will not dull or corrode

heat sterilization

5

can be used for heat sensitive materials but SLOW (10-16 hours)

ethylene oxide
for heat sensitive materials

6

is paterurization sterilization or disinfectant?

only a disinfectant!

7

what time and temp should you use for autoclave?

121 C for 15 - 20 mins at 15 psi
check with bacterial spores

8

pass through small pores that bacteria are excluded – technically not sterile

filters

9

UV light – thymine dimers (hoods, food prep areas); gamma (gloves)

radiation

10

in a _____ culture, multiple organisms grow but look different?

differential

11

in this culture, organisms are enriched for unique metabolic needs?

specialized

12

what kind of media will inhibit the growth of some?

selecitve
differential wil alom the ID of certain types of organisms

13

why might a look at the colony morphology be useful?

might help with ID. circular, spindle, raised, etc.

14

what color with the hemolysis of ALPHA?

green!

15

beta hemolysis color

CLEAR_ completely cleared cuz it's BETTA

16

____ hemolysis is NONE

gamma-cuz gamma sorority girls are dumb

17

rings shift phase of light; live specimens and internal structures

phase contrast

18

two lenses, often need to stain prep to view

brightfield

19

when do we use the acid fast stain?

mycobacterium

20

what do the fluorescent stains let us see?

surface antigens and structures