Flashcards in Handout 4 Deck (20):
- formalin (37% in water) - denatures proteins and nucleic acids – alkylating agent
ethylene oxide – for heat sensitive materials
1) 2% gluteraldehyde – alkylated DNA and proteins
put in the chemiclve with formaldehyde and a little water
Dry heat 320F for 2 hours or 340F for 1 hour. Coagulates proteins – safe for metal will not dull or corrode
can be used for heat sensitive materials but SLOW (10-16 hours)
for heat sensitive materials
is paterurization sterilization or disinfectant?
only a disinfectant!
what time and temp should you use for autoclave?
121 C for 15 - 20 mins at 15 psi
check with bacterial spores
pass through small pores that bacteria are excluded – technically not sterile
UV light – thymine dimers (hoods, food prep areas); gamma (gloves)
in a _____ culture, multiple organisms grow but look different?
in this culture, organisms are enriched for unique metabolic needs?
what kind of media will inhibit the growth of some?
differential wil alom the ID of certain types of organisms
why might a look at the colony morphology be useful?
might help with ID. circular, spindle, raised, etc.
what color with the hemolysis of ALPHA?
beta hemolysis color
CLEAR_ completely cleared cuz it's BETTA
____ hemolysis is NONE
gamma-cuz gamma sorority girls are dumb
rings shift phase of light; live specimens and internal structures
two lenses, often need to stain prep to view
when do we use the acid fast stain?