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Flashcards in Handout 5 Deck (21):
1

advantages to using molecular techniques for the analysis of infectious agents?

SSS
sensitivity
safety
speed

2

what is the copy machine for DNA?

PCR

3

what is restriction fragment length polypmorphism used for? example from class?

the military example of sequencing a strain
finger printing

4

what is the method that detects PATHOGEN in the DNA sample?

DNA hybridizaiton

5

what uses a probe to look for pathogen DNA in a fixed sample?

In SITU hybridization

6

AKA fingerprinting?

RFLP

7

what are you detecting in serological testing?

ANTIBODY!

8

soluble molecules forming antibody/antigen complexes?

precipitation

9

clumping larger antigens like CELLS?

agglutination

10

what uses antibodies to look for markers on cells/separate cells?

flow cytometry

11

what uses antibodies to detect antigen?

ELISA

12

what identifies proteins or antibodies to a protein?

western blot

13

what is similar to ELISA but uses radiation?

RIA!

14

what is the microbiological approach that ID based on antigens, DNA or other molecular techniques?

immunological/molecular

15

what is the old schools approach that uses the 5 Is?

pure microbiological appraoch.
innoculation, incubation, isolation, inspection, identificaiton

16

disease contracted form a health care setting?

Iatrogenic

17

unknown cause?

idiopathic

18

infection causing severe systemic inflammatory reaction?

septicemia

19

membranes covering brain and spinal cord?

meninges

20

inflammation in membranes covering the brain and the spinal cord?

meningitis

21

MIC?

animicrobial susceptiility test
minimum inhibitory concentration