Hematology Lab Part 1

Question 1

Functions of Blood

Answer 1

Provides a medium for the maintenance of homeostasis in the cell’s environment Functions as a transportation system, bringing nutrients and oxygen to the cells and removing wastes and carbon dioxide from interstitial fluid around the cells Integrates various organs through hormones Provide buffers for acid-base balance Destroys foreign organisms through phagocytosis and antibody action Provide distribution of body heat & heat conservation Prevent its own loss through hemostasis (coagulation) mechanisms

Question 2

The percent volume of whole blood that is occupied by red blood cells

Answer 2

Hematocrit

Question 3

Determined by comparing the height of the red cell in the centrifuged tube to the height of the total column of blood

Answer 3

Blood Hematocrit

Question 4

Normal values of male Hematocrit values and range

Answer 4

Average = 46% Range = 43 - 49%

Question 5

Average = 46% Range = 43 - 49%

Answer 5

Males Hematocrit Levels

Question 6

Average = 41% Range = 36 - 45%

Answer 6

Females Hematocrit Levels

Question 7

Severe Anemia

Answer 7

Hematocrit may fall to as low as 15%

Question 8

Hematocrit may fall to as low as 15%

Answer 8

Severe Anemia

Question 9

Normal Values of Females Hematocrit & Range

Answer 9

Average = 41% Range = 36 - 45%

Question 10

Hematocrit

Answer 10

The percent volume of whole blood that is occupied by red blood cells

Question 11

Polycythemia

Answer 11

Hematocrit may rise to as high as 70%

Question 12

Hematocrit rises to as high as 70%

Answer 12

Polycythemia

Question 13

Calculating Hematocrit

Answer 13

Hct (%) = height of red cells (mm) / height of red cells and plasma (mm) x 100

Question 14

Amount of O2 which blood can carry is closely related to

Answer 14

Concentration of hemoglobin

Question 15

Each gram of hemoglobin when completely saturated is capable of carrying

Answer 15

1.34 ml of oxygen

Question 16

34% of the red blood cell by weight is

Answer 16

Hemoglobin; it is essentially a bag of hemoglobin

Question 17

Average = 15.4 g/100 ml blood Range = 13.6 to 17.2

Answer 17

Average concentration/Range of Hemoglobin in Males

Question 18

Average: 13.3 g/100 ml blood Range: 11.5 to 15.0

Answer 18

Average concentration/Range of Hemoglobin in Females

Question 19

Anemic

Answer 19

Less than 10g/100ml of blood

Question 20

Less than 10g/100ml of blood

Answer 20

Anemic

Question 21

Calculation of Total Maximum Oxygen Carrying Capacity

Answer 21

Total blood Hb (g) = blood volume (ml of blood) X hemoglobin concentration (g Hb/ml of blood) Total oxygen carrying capacity = (total g Hb) X (1.34 ml O2/g Hb)

Question 22

Each gram of hemoglobin is capable of carrying

Answer 22

1.34 ml of oxygen when completely saturated

Question 22

Strenuous exercise, rheumatic fever, severe burns

Answer 22

Neutrophilic Leukocytosis

Question 23

Blood Volume for Females

Answer 23

65 ml blood per kilogram body weight +/- 10% (1 kg = 2.2 lbs)

Question 24

Differential white blood cell count

Answer 24

The percentage of each type of white cell in the total leukocyte population is calculated

Question 25

Average concentration/range of hemoglobin in Males

Answer 25

Average = 15.4 g/100 ml blood Range = 13.6 to 17.2

Question 26

Neutrophilic Leukopenia

Answer 26

Protozoan infections, malnutrition, aplastic anemia

Question 27

Neutrophilic Leukocytosis

Answer 27

Strenuous exercise, rheumatic fever, severe burns

Question 28

Scarlet fever, parasitic infections, allergic reactions

Answer 28

Eosinophilia

Question 29

Average concentration/Range of Hemoglobin in Females

Answer 29

Average: 13.3 g/100 ml blood Range: 11.5 to 15.0

Question 30

Protozoan infections, malnutrition, aplastic anemia

Answer 30

Neutrophilic Leukopenia

Question 31

Lymphocytosis

Answer 31

Mumps, German measles, whooping cough

Question 32

Blood volume for Males

Answer 32

79 ml blood per kilogram body +/- 10%

Question 33

Mumps, German measles, whooping cough

Answer 33

Lymphocytosis

Question 34

Eosinophilia

Answer 34

Scarlet fever, parasitic infections, allergic reactions

Question 35

Monocytosis

Answer 35

Chronic diseases, such as tuberculosis & leukemia

Question 36

Granulocytes

Answer 36

Neutrophils Eosinophils Basophils

Question 37

Chronic diseases, such as tuberculosis & leukemia

Answer 37

Monocytosis

Question 38

Lymphocytopenia

Answer 38

Administration of glucocorticoid drugs

Question 39

Administration of glucocorticoid drugs

Answer 39

Lymphocytopenia

Question 40

Eosinophils

Answer 40

2-4% of total WBC 13 microns diameter Bilobed nucleus Coarse red-orange cytoplasmic granules, blue-purple nucleus

Question 41

Neutrophils Eosinophils Basophils

Answer 41

Granulocytes

Question 42

Neutrophils

Answer 42

65% of total WBC 10-12 microns diameter Multi-lobed (3 or more) nucleus Small pink cytoplasmic granules, purple nucleus

Question 43

Small Lymphocytes

Answer 43

20-25% of total WBC 7 microns diameter Very large, spherical nucleus surrounded by a thin cytoplasm Light blue cytoplasm (nongranular), deep blue or purple nucleus

Question 44

65% of total WBC 10-12 microns diameter Multi-lobed (3 or more) nucleus Small pink cytoplasmic granules, purple nucleus

Answer 44

Neutrophils

Question 45

2-4% of total WBC 13 microns diameter Bilobed nucleus Coarse red-orange cytoplasmic granules, blue-purple nucleus

Answer 45

Eosinophils

Question 46

Basophils

Answer 46

0.5% of total WBC 14 microns diameter Bilobed nucleus Black nucleus is usually completely obscured by large, deep-blue or reddish-purple cytoplasmic granules

Question 47

0.5% of total WBC 14 microns diameter Bilobed nucleus Black nucleus is usually completely obscured by large, deep-blue or reddish-purple cytoplasmic granules

Answer 47

Basophils

Question 48

Agranulocytes (Mononuclear Leukocytes)

Answer 48

Small Lymphocytes Large Lymphocytes Monocytes

Question 49

20-25% of total WBC 7 microns diameter Very large, spherical nucleus surrounded by a thin cytoplasm Light blue cytoplasm (nongranular), deep blue or purple nucleus

Answer 49

Small Lymphocytes

Question 50

Another name for Granulocytes

Answer 50

Polymorphonuclear Leukocytes

Question 51

Large Lymphocytes

Answer 51

3% of total WBC 10 microns diameter Large oval, indented nucleus Light blue cytoplasm (nongranular), dark purple nucleus

Question 52

Erythrocyte

Answer 52

Another name for a RBC

Question 53

Monocytes

Answer 53

3-7% of total WBC 15 microns diameter Large, deeply indented nucleus (horseshoe-shaped) Light blue-gray cytoplasm (nongranular), blue or purple nucleus

Question 54

3-7% of total WBC 15 microns diameter Large, deeply indented nucleus (horseshoe-shaped) Light blue-gray cytoplasm (nongranular), blue or purple nucleus

Answer 54

Monocytes

Question 55

Normally, each 100 mL of blood will contain approximately:

Answer 55

15 grams of hemoglobin, which is distributed among the 500 billion red cells in each 100 mL of whole blood

Question 56

Another name for red blood cell

Answer 56

Erythrocyte

Question 57

3% of total WBC 10 microns diameter Large oval, indented nucleus Light blue cytoplasm (nongranular), dark purple nucleus

Answer 57

Large Lymphocytes

Question 58

In our lab we measured our hemoglobin concentration in

Answer 58

% = g Hb/100 ml of blood

Question 59

Major difficulties seldom develop until

Answer 59

level of 7.5g/100ml of blood is reached

Question 60

A rat has approximately how much blood per kilogram?

Answer 60

70 ml blood per kilogram body weight

Question 61

average leukocyte count

Answer 61

7,500/mm^3 average

Question 62

Another name for Agranulocytes

Answer 62

Mononuclear Leukocytes

Question 63

Function of Heparin

Answer 63

inhibits coagulation