Hematology Lab Part 2 Flashcards Preview

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Flashcards in Hematology Lab Part 2 Deck (39):
1

The blood contains 3 specialized classes of cells or "formed elements"

Red blood cells (erythrocytes)
White blood cells (leukocytes)
Platelets (thrombocytes)

2

Another name for RBC

Erythrocytes

3

Another name for WBC

Leukocytes

4

Another name for Platelets

Thrombocytes

5

The function of these cells are to transport oxygen and carbon dioxide

RBC

6

The function of these cells are to combat infections and invading organisms

WBC

7

The function of these cells are to prevent blood loss

Platelets

8

Normal Blood Cell Values of RBC in Males

5.4 +/- 0.8 million per cubic millimeter (mm^3)

9

Normal Blood Cell Values of RBC in Females

4.8 +/- 0.6 million per cubic millimeter (mm^3)

10

Normal Blood Cell Values of WBC in Males

7-9000 per mm^3

11

Normal Blood Cell Values of WBC in Females

5-7000 per mm^3

12

Normal Blood Cell Values of Platelets

150,000 to 400,000 per mm^3
average = 300,000 per mm^3

13

Which are not true "cells"?

Erythrocytes and the Platelets;
They both lack nuclei and are unable to undergo mitosis to form daughter cells;
nothing more than "bags" to carry specific chemicals;
hemoglobin in RBC & platelet factor 3 in the platelets

14

If each gram of hemoglobin in the RBC is maximally saturated with oxygen, it can carry about

1.34 mL of oxygen

15

In each 100 mL of blood there is roughly

15 g of Hb, hence around 20 mL of oxygen can be carried in every 100 mL of blood

16

Anemia often results from

an abnormal decrease in the number of erythrocytes, so that insufficient oxygen is carried to the tissues and they become oxygen starved
other factors may also cause anemia, such as decreased Hb in each cell, decreased cell size, hemorrhage, etc.

17

In order to accurately diagnose the cause of anemia the total erythrocyte picture must be examined

hematocrit, hemoglobin, RBC count, cell size, hemoglobin in each cell, etc

18

Automatic device that makes cell counts

Coulter Counter

19

Hemocytometer

Each of the 2 counting chambers for counting blood cells are
9 mm squares divided into 9 squares, each measuring 1 mm square;
4 corner squares are used for counting leukocytes and are divided into 16 smaller squares
The center 1 mm^2 square is divided into 25 small squares (1/25 mm^2), and each is further subdivided into 16 smaller squares

20

Leukopenia

Deficient number of leukocytes

21

Leukocytosis

Abnormally high number of leukocytes

22

Which chemical do RBC carry?

Hemoglobin

23

Which chemical do platelets carry?

platelet factor 3

24

Tool we used in our blood lab for diluting

Thoma Pipettes

25

Purpose of Hayem's or Gower's solution

preserve the corpuscles and prevent coagulation

26

The dilution of RBC was by a factor of

200X

27

The depth of the counting chamber for RBC was by a factor of

10X

28

The area of the RBC was multiplied by a factor of

25X

29

The total multiplication factor for RBC is

200 X 10 X 25 = 50,000

30

If you were to count an average of 120 RBC per 1/25 mm^2 square, your RBC count would be

120 X 50,000 = 6,000,000 RBC per mm^3

31

By calculating the RBC size and the percentage of hemoglobin each cell carries, we are able to more accurately pinpoint the cause of

Anemia

32

Mean Corpuscular Volume (MCV)

MCV = hematocrit (% red cells) X 10 / RBC count (millions/mm^3)

33

Normal Range of MCV

87 +/- 2 cubic microns

34

Mean Corpuscular Hemoglobin Concentration (MCHC)

MCHC (%) = hemoglobin (g/100ml blood) X 100 / hematocrit (%)

35

Normal Range of MCHC

34 +/- 2% of the RBC is hemoglobin

36

The WBC were diluted by a factor of

X20

37

The WBC depth was by a factor of

X10

38

The average number of cells in 1 mm^2 were counted by a factor of

X1

39

The total multiplication factor for WBC

20 X 10 X 1 = 200