JVD 2019 #2 Use of Platelet-Rich Plasma and Platelet-Rich Fibrin in Dentistry & Oral Surgery: Introduction and Review of the Literature; Feigin, Shope Flashcards
Growth factors in platelets are responsible for….
Increased cell mitosis Increased collagen synthesis Blood vessel growth Cellular recruitment Other factors important for healing and regeneration of tissues
What are platelet concentrates?
Concentrated suspensions of growth factors in platelets that act as locally applied bioactive surgical additives to induce wound healing
What is platelet rich plasma?
Concentrate of PRP protein obtained from whole blood and centrifused to remove the RBCs
What is platelet rich fibrin?
2nd-generation platelet-rich concentrate where autologous platelets and leukocytes are present in a complex fibrin matrix
What occurs in the 1st centrifugation?
The second?
1- blood separates into 3 distinct strata (red corpuscules base, acellular plasma (PPP) and Max platelet concentration area)
2- platelet concentration at the bottom of the tube
cPRP: the platelet concentrate is suspended in an acellular plasma fraction
What is the lifespan of inactivated platelets?
7-10 days
How can platelet-rich fibrin be used?
Resorbable membrane for GTR as bioscaffold
What are the known cytokines & growth factors in platelet-rich fibrin?
interleukin 1B (IL-B), IL-4, tumor necrosis factor alpha, PDGF A and B, TGF-B1, insulin-like growth factor 1, vascular endothelial growth factor (VEGF).
TGF-B1, PDGF-AB and VEGF released from PRF in highest amounts.
Has sustained release of growth factors for 1-4 weeks.
Take-home message for Protocol for PRP preparation
8mL blood= 1ml PRP
1st spin= hard= separate the RBCs from plasma that contains platelets, WBCs and some clotting factors (buffy coat)
2nd spin= soft= separate platelet concentrate (PRP) from the platelet-poor plasma (PPP)
Use in 4 hours.
Release of growth factors begins within 10mins, almost 95% secreted in 1st hour
