JVD 2020 #4 Biphasic Calcium Phosphate Microparticles Mixed with Autologous Blood: Application for the Reconstruction of a Large Mandibular Bone Defect in a Dog; Girard

Question 1

In prior combined surgical and regenerative method for reconstruction of critical size mandibular defects in dogs, what was used?

Answer 1

Collagen sponge containing granules of hydroxyapatite (HA) and beta-tricalcium phosphate infused with rhBMP. (Arzi 2015)

Question 2

What is BCP, biphasic calcium phosphate?

Answer 2

BCP is a combination of hydroxyapatite (HA) and beta-tricalcium phosphate (B-TCP) in the form of ceramic granules or combined with gel-polymers

Question 3

How does the combination of HA and B-TCP work?

Answer 3

HA is not resorbed and inert, thus provides a structural scaffold. B-TCP is rapidly resorbed, has bioactivity for osteoinduction and promotes tissue regeneration.

Question 4

What portion of blood was found to be responsible for induction of new bone formation in prior study of clotted whole blood with BCP microparticles?

Answer 4

The mononuclear cell fraction.

Question 5

What did the CaP biomaterial in BRB consist of?

Answer 5

calibrated 80-200 micrometer microporous microparticles of BCP, composed of 60% HA and 40% B-TCP in a ready-to-use kit

Question 6

Was any fixation used to adhere the gel form BRB cylinder or clotted composite to the bone ends or plate?

Answer 6

No. It was laid alongside the plate without any rigid fixation to the plate or bone. The surroundign soft tissue were sutured to provide soft tissue cover to the BCP/blood and implant plate.

Question 7

When was evidence of new bone bridging the interface between the implant material and the adjacent mandibular bone first observed?

Answer 7

At the 4 week-post surgery CBCT.

Question 8

What are the objectives of bone substitutes?

Answer 8

serve as a substrate for the migration, proliferation and differentiation of cells originating from surrounding tissues, followed by bone ingrowth.