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Flashcards in Lab characteristics Deck (54)
1

Giemsa Stain (5)

Chlamydia, Borrelia, Rickettsiae, Trypanosomes, Plasmodium (Certain Bugs Really Try my Patience)

2

PAS Stain

Stains glycogen, mucopolysaccharides; used to diagnose Whipple disease (Tropheryma whipplei)

3

Ziehl-Neelsen (carbol fuchsin)

Acid fast organisms (Nocardia, Mycobacterium)

4

India Ink

Cryptococcus neoformans (mucicarmine can also be sued to stain thick polysaccharide capsule red)

5

Silver stain

Fungi (ex: Pneumocystis), Legionella, Helicobacter pylori

6

H. influenzae culture

Chocolate agar with factors V (NAD+) and X (hematin)

7

Neisseria

Thayer-Martin (or VPN) media - vancomycin, polymyxin, and nystatin)

8

Bordetella pertussis

Bordet-Genou (potato) agar (Bordet for Bordetella

9

Cornyebacterium diptheriae

Tellurite agar, Loffler medium

10

M. tuberclosis

Lowenstein-Jensen agar

11

M. pneumoniae

Eaton agar; requires cholesterol

12

Lactose-fermenting enterics (5)

Citrobacter, Klebsiella, E. coli, Enterobacter, Serratia (weak)

13

Legionella

Charcoal yeast extract agar buffered with cysteine and iron

14

Fungi

Sabouraud agar

15

Obligate aerobes (3)

Nocardia, Pseudomonas aeruginosa, Mycobacterium tuberclosis

16

Obligate anaerobes (3)

Clostridium, Bacteroides, Actinomyces

17

Obligate intracellular (2)

Rickettsia, Chlamydia

18

Facultative intracellular (8)

Salmonella, Neisseria, Brucella, Mycobacterium, Listeria, Francisella, Legionella, Yersinia pestis

19

Encapsulated bacteria (7)

Strep pneumo, Haemophilus influenzae type B, Neisseria meningitidis, E. coli, Salmonella, Klebsiella pneumoniae, and Group B Strep (SHiNE SKiS)

20

Catalase (+) organisms (7)

Pseudomonas, Listeria, Aspergillus, Candida, E. coli, S. aureus, Serratia (PLACESS for your cats)

21

Urease (+) bugs (8)

Cryptococcus, H. pylori, Proteus, Ureaplasma, Nocardia, Klebsiella, S. epidermidis, S. saprophyticus

22

Yellow pigment

S. aureus, Actinomyces israelli (sulfur granules)

23

blue-green pigment

pseudomonas aeruginosa

24

red pigment

serratia marcescens

25

protein A virulence factor

s. aureus

26

IgA Protease

S. pneumoniae, H. influenzae type B, Neisseria (SHiN)

27

M protein

Helps prevent phagocytosis; Group A Strep

28

Protein A

Binds Fc regionof IgG; S. aureus

29

Novobiocin differentiates what?

Staph epidermidis (sensitive) from Staph saprophyticus (resistant)

30

Bacitracin differentiates what?

Strep pyogenes (sensitive) from Strep agalactiae (resistant)

31

Optochin differentiates what?

Strep pneumo (sensitive) from Viridans streptococci (resistant)

32

Alpha hemolytic (2)

Strep pneumo, Viridans streptococci

33

Beta hemolytic (4)

Strep pyogenes, Strep agalactiae, Staph aureus, Listeria monocytogenes

34

Gamma hemolytic (no hemolysis)

Enterococci (Group D), Strep bovis

35

Oxidase (+) organisms

Pseudomonas, Campylobacter jejuni, V. cholerae, H. pylori

36

Produces H2S

Salmonella

37

Grows at 42 degrees C

Campylobacter jejuni

38

Grows in alkaline media

Vibrio cholerae

39

hippurate test +

s. agalactiae

40

elek test

C. diphtheriae

41

spore forming

b. anthracis, c. perfringens, c. tetani, b.cereus, c.botulinum, coxiella burnetii

42

interferon-g release assay

TB

43

pink colonies on macconkey agar

Citrobacter, Klebsiella, E. coli, Enterobacter, Serratia (weak)

44

EMB agar

lactose fermenters (purple/black), E.coli (purple w/green sheen)

45

chocolate agar

h.influenzae, s. aureus

46

charcoal yeast extract culture with iron and cysteine

legionella

47

light microscopy with aniline dyes

borrelia

48

dark field microscopy

treponema

49

VDRL/RPR

syphillis, false positive: viruses (EBV, hep), drugs, SLE, leprosy

50

eaton agar

mycoplasma pneumoniae

51

scotch tape test

enterobius vermicularis

52

leukocyte esterase test positive

bacterial uti

53

nitrite test +

gram negative uti

54

negative urease test for a UTI

E.coli, enterococcus