Lab Midterm Study Guide

Question 1

How is lactose broken down

Answer 1

lactaid

Question 2

What are the products of lactose breakdown

Answer 2

glucose and galactose

Question 3

what are the products of ONPG breakdown

Answer 3

glucose and DNP

Question 4

Why did the tube turn yellow in the lactaid experiment ?

Answer 4

separation from ONPG into glucose and DNP

Question 5

what is an example of a control variable ?

Answer 5

water

Question 6

what was the independent variable in lab 1 ?

Answer 6

lactaid

Question 7

what was the dependent variable in lab 1 ?

Answer 7

color

Question 8

experimental group and control group, what’s the difference

Answer 8

experimental group receives the treatment, the control group is not changed

Question 9

Benedict’s Reagent is used to test for _____

Answer 9

monosaccharides/sugars

Question 10

What color does Benedict’s Reagent turn when positive

Answer 10

Green (low), yellow, orange, red (high)

Question 11

What color does Benedict’s Reagent turn when negative

Answer 11

Baby Blue

Question 12

What is a positive control

Answer 12

Substance that contains molecule that is being tested

Question 13

What is a negative control

Answer 13

Substance that does not contain the molecule being tested

Question 14

Iodine is used to test for the presence of ____ which is a ___

Answer 14

amylose : starch

Question 15

Positive result of the Iodine test is

Answer 15

Blue/Black Color

Question 16

Negative result of the Iodine test is

Answer 16

Pale Yellow Color

Question 17

Amylose chemically digests ____ in crackers

Answer 17

starch

Question 18

Sudan IV Dye and Greasy Spot Test for the presence of _____

Answer 18

oils / triglycerides

Question 19

Sudan IV Test is what color when positive

Answer 19

Red

Question 20

Sudan IV Test is what when it’s negative

Answer 20

Not fully mixed, particles will float

Question 21

What are detergents used for ?

Answer 21

Help dissolve oils in water through EMULSIFICATION

Question 22

If the paper in the Greasy Spot Test dries within minutes it is

Answer 22

Aqueous

Question 23

If the paper in the Greasy Spot Test is still wet after a few minutes, their are signs of ___

Answer 23

lipids

Question 24

The Biuret test is used to test for ____

Answer 24

Proteins

Question 25

The Biuret Test is ___ when it is a negative result

Answer 25

Blue

Question 26

The Biuret Test is ____ when it is a positive result

Answer 26

Purple/Violet

Question 27

The Dih Diphenylamine Test binds ____ and _____ to form a molecule

Answer 27

DNA and Diphenylamine

Question 28

The Dih Diphenylamine test is ___ when positive

Answer 28

blue

Question 29

The Dih Diphenylamine test is ___ when negative

Answer 29

no change

Question 30

What does the iris diaphragm do

Answer 30

Limits the amount of light transmitted

Question 31

Stage Knob

Answer 31

Moves the stage left to righty

Question 32

Coarse Focus Knob

Answer 32

Moves the stage up and down

Question 33

Fine Focus Knob

Answer 33

Focuses the stage quality

Question 34

What is Microscopy

Answer 34

Study of Microbes using a Microscope

Question 35

Micrometer Size Range

Answer 35

1 micrometer = 1 / 1000 millimeter

Question 36

Magnification

Answer 36

Amount of enlargement of an image

Question 37

Resolution

Answer 37

Ability to see detail

Question 38

Clarity

Answer 38

Crispness of the image

Question 39

Formula for FOV

Answer 39

FOV low * Mag Low = FOV high * Mag High

Question 40

FOV Area Formula

Answer 40

A = pi(r)^2