Pre Lab Quiz 14-15-16 Flashcards
DNA Fingerprinting
Technique used to identify an individual based on DNA sequence
Regions of DNA that code for proteins
Coding Regions
Polymorphic DNA
Refers to chromosomal DNA that varies widely
DNA can be identified by ___ or ____
RFLP or PCR
Restriction Endonucleases
enzymes that cut DNA in a sequence and generate fragments
STR
FBI uses it, microsatellites
VNTR
minisatellite repeats, 100+ times
RFLP
differentiation between two individuals
Gel Electrophoresis
Used to separate DNA fragments and visualize them
What does Gel Electrophoresis use to seperate DNA fragments
Agarose Gel
DNA placed in electric current will move toward electrode ___-
anode
The agarose gel forms a porous ___
lattice
DNA ladder
A standard made up of several dna molecules of dif of known lengths
Transformation
phenotypic change caused by change in genotype
Gene
sequence of DNA providing instructions for making a protein
Gene sequence is converted into mRNA by process called ___
transcription
Transform bacteria with ___ gene codes for Green Flourescent Protein
GFP green flourescent protein
GFP gene is introduced into bacteria using ___ ____ ____
recombinant DNA plasmid
plasmid is reffered to as a ___
vector