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Flashcards in M19 Deck (40):
1

what is disinfection

process by which the number of microorganism are reduced to a level that is considered safe

2

what ca still be present after disinfection

bacteria
spores
viruses

3

what is antiseptic

antimicrobial agent that can be applied safely to the surface of th body or exposed tissues

4

what re some common routes of disinfection

cleaning
heat
ultrasound
chemicals

5

what does cleaning do

reduce bioburden

6

what are the ways of cleaning

manual auomated
hot water nd detergent
damp dust

7

what are some heat methods of disinfection

pasteurisation
boil water
wash and rinse

8

what does ultrasound do to disinfect

high frequency sound waves
remove dirt
disrupt cell mem
prep o autoclave

9

what are some chemicals used to reduce microbes on skins nd mucous mems

alcohols
centirmide
chlorhexidine
iodine

10

what are the features of alcohol

wet contact 3 mins
kill bac
inactivate spores
evap, flam
caotropic

11

what is centirmide

low level disinfectant
quaternary ammonium compounds
cationic detergents

12

what can inactivate centrimide

anionic detergents
soap and water

13

what is common use of chlorhexidine

hibiscrub hand disinfection

14

what is a common use of iodine

betadine
surgical scrub

15

what is a iodophor

iodine plus detrgent

16

what are some chemicals used to reduce microbes on inanimate objects

hypochlorites
phenols
alcohols

17

what are hypochlorites

halogen compound
chlorine based

18

what is hypochlorites weak ag

tbuercolcidal activity

19

what are the strong and was hypochlorite solutions used for

strong - visible blood contemn expo for 2 mins
weak - no blood contemn expo for 10 min

20

what do phenols do

denature proteins and disrupt cell mems
spores resist inactivation
hycolin 1%

21

when using disinfectants what is to be considered

range antimicrobial activity
rate of action
kind of action
effect of pH
stability
contact time

22

what is sterilisation

process by which all microorganisms are killed or removed to render the object incapable of causing infection

23

what is classified as sterile

unable to reproduce sexually
free form living microorganisms
1 x 10^6

24

what re some methods of sterilisation

heat
chemical
radiation
filtration

25

what depicts the choice of sterilisation method

purpose
nature of material
nature of contamination
convenience - time

26

what si the spectrum of microorganisms from sensitive to resistant

viruses
bac cells
fungal spores
bac spores
prions

27

what is the D value

time taken to reduce the viable number of organisms by 90%

28

what is bioburden

num bac present

29

Nature of sterilisation media

proteinaceous org mat increased chance of survival

30

wat are the diff heat for sterilisation

direct
dry heat
moist heat

31

what is dry heat

hot air oven
kills by destroy oxo of essential cell constituents

32

what si moist heat

kills by coag and denture enzyme and structural protein
autoclaving

33

wha do u do for an autoclave

in container load
steam enter
remove air
close valves
stea pressure of set time
turn off hat
allow pressure to fall

34

what is the requirements of autoclaving

2.25 bar
134 degrees
3 mins

35

what are the controls for heat sterilisation

record sheet and probe
browns tube
autoclave tape
spore strips

36

what does formaldehyde vapour do

fumigation
lack good penetration
sterilising heat

37

what does 2 % gluteraldehyde do

buffered to alkaline pH
inactivated by organic material

38

how does ultraviolet sterilise

low energy
good penetration
burns skin
reduces bac count

39

how'd does ionising radiation sterilise

high energy
good penetration
carcinogenic
cobalt 60 source

40

whats the way filtration is used to sterilise

mechanical process
size exclusion
high efficiency particulate air filters