M2 Lab Methods Flashcards Preview

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Flashcards in M2 Lab Methods Deck (35):
1

what is procedure

handling specimen
lab processing and reporting
interpretation and use of info

2

what do u do in lab analysis

isolate and identify organism
- sample
- timing
- handling
- request form

3

how is a sample taken fro purulent infection (pus forming)

aspirate of pus - drain
prevent contamination
maintain anaerobes

4

how to sample mucosal infections

yeast, swab and oral wash
herpes, lesions swab

5

how to sample caries and per disease

complex pic involving mix of organisms

6

how to do samples for culture

results = 2-5 days
streaked blood agar (aerobic/anaerobic)
colonies then indie cosines
antibiotic sensitivity

7

what does culturing do

determine viability (aerobes Vs anaerobes)
component species
provides pure material (single colony)

8

what is culturing sequence

primary inoculum
second streak
3rd streak
streak to single colonies

9

what is general media

transport media
- sloppy agar, incl charcoal prevents desiccation and abs toxic agents

rich excess of nutrients

10

what is enrichment media

addition of blood/serum/extracts will support growth of fastidious organisms
blood agar also - haemolytic profile determined

11

what si selective media

presence of specific substance permits the growth of one organism over another

12

what re some examples of selective media

mannitol salt agar
salmonela-shigella

13

what is differential media

incroportio of chems produces visible changes in colonies that facilitate identification

14

what are some examples of differentiate media

macconkay agar
- bile salts & crystal violet
eosin & methylene blue

15

what can be used for identification

microscopy - single colony or pus sample
- pure culture
- shape, size, grouping
-structures
- staining

16

what are the cocci structures

single
pair
cluster
chains

17

what are the rod bacilli structure

single
chain
curved rod
spiral

18

what is one species with many colony phenotypes

bacillus subtilis

19

what is metabolic profiling

biochem characterisation
utilisation of carbon sources and amino acids

20

what is exo enzyme production

catalase
coagulase
hydrolysis of lipid
urease

21

whats urease

urea converted to ammonia & CO2

22

what causes fermentation

lactose

23

what are serological tests

host immune response to Ag by rising of Abs

24

what are Ab specific to

microbe or single component

25

what else does serological tests do

detects presence of sp IgM Ab to virus
demo in vitro bu agglutination
rapid detection of viruses
serotype bac indeitifacton

26

what are immunological techniques

difficult to identify pathogens
artificially raised sp antibodies
- agglutination reactions
- DFA test
- ELISA

27

what is aggltination

antibody to antigen with latex bead - red blood cell
caring clot

28

what is DFA fluorescence tect

one step - org expo to Ab with attached dye, organism bind toAb and be seen

two step - similar to one step Ab at start not dye, instead secondary Ab dyed

29

what is ELISA

enzyme linked immunosorbent assay

30

how do u do ELISA

coat wells with antigen
wash
add antibody test
wash
add enzyme conjugate
wash
add chromogenic enzyme substrate and read colour change

31

what are virulence test

virulence factor can be critical in pathogenesis (adhesion, toxin)
in vitro antibody recognition, DNA hybridisation

32

what is DNA technology

sp primers amplify sp peice dna
PCR

33

what is MALDI-TOF

matrix assisted laser desorption ionisation time of fight

34

what are the features of MALDI_TOF

powerful
rapid
precise
cost effective

35

what does madli tof do

gen series of ions from a sample dept on its constituents
sep ions accord to mass
detects proteins
results in characteristic signature