Microbio 3rd Exam Flashcards Preview

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Flashcards in Microbio 3rd Exam Deck (38)
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1
Q

oxytrophic mutant, deficient in some metabbolic function
grow mutants on media containing the missing ingredient and media lacking it
if they can grow on media that lacks it, it means they mutated and lacks gene of interest

A

.

2
Q

cDNA, when you deal with eukaryote, the RNA is complete because introns have been removed,

A

.

3
Q

fluorescent probe, only hybridizes with complementary

differs from western blot, id specific proteins, southern for gene sequences

A

.

4
Q

7) cell engulfing prokaryotic organisms

derived mitochondria and chloroplasts

A

.

5
Q

biochemical tests are used to determine

A

enzymatic activity

6
Q

why aren’t viruses classified in a kingdom?

A

the do not have cells

7
Q

Which scientists proposed the kingdom prokaryote in 1968?

A

Murray.

8
Q

Bacteria and arachaea share which characteristic?

A

They are both prokaryotic cell types.

9
Q

Into which group would you place a single-celled photoautotroph?

A

Protist.

10
Q

Which one of the following statements describes the domain Bacteria?

A

They have prokaryotic cells

11
Q

Which domain has linear chromosomes?

A

Eukarya

12
Q

Which one of the following do not apply to eukaryotic cells?

A

Have circular chromosomes.

13
Q

Prokaryotes have 70s and Eukaryotes have 80S Ribosomes

A

.

14
Q

Treating DNA from different bacteria with the same restriction enzyme followed by separation of the fragments by electrophoresis is known as

A

DNA fingerprinting.

15
Q

Organisms that can form thin tubes called hyphae

A

Fungi

16
Q

In the scientific name staphylococcus aureus, Staphylococcus is the?

A

Genus.

17
Q

Historically, eukaryotic organisms that did not fit into other kingdoms were placed into?

A

Protista

18
Q

Cladograms for microorganisms are made from information derived from?

A

rRNA sequences.

19
Q

Fossil evidence indicates that cyanobacteria-like cells first existed on Earth how many years ago?

A

3.5 billion.

20
Q

A molecular clock for evolution is based on

A

Nucleotide sequences in the genomes of organisms.

21
Q

Which of the following is the correct order in the taxonomic hierarchy from the broadest to most specific

A
  • King
  • phy
  • class
  • order
  • family
  • genus
  • species
22
Q

Unlike Archaea and Eukarya, the first amino acid in protein synthesis in bacteria is.

A

Formylmethionine

23
Q

Genetically related groups of eukaryotes that share a common ancestor are known as?

A

Clades.

24
Q

Which one of the following is not a major group of archaea?

A

Obligate acidophiles

25
Q

Which of the following techniques can ID antibodies in a patient’s serum.

A

Western blotting.

26
Q

To which kingdom do molds belong?

A

Fungi

27
Q

The test that involves a reaction between known antibodies and an unknown bacterium is known as

A

ELISA.

28
Q

Phage typing - known virus between unknown bacterium

A

.

29
Q

A disease acquired by many people in an area in short time

A

epidemic

30
Q

In london drank broad street pump

A

john snow

31
Q

Germ-free animals

A

have increased susceptibility to disease.

32
Q

In commensalism

A

one organism benefits, the other is unaffected.

33
Q

An example of a non-communicable disease?

A

Tetanus.

34
Q

Microbial antagonism

A

preventing overgrowth of harmful microorganisms in host.

35
Q

Prodromal period

A

stage of disease that is early, mild symptoms.

36
Q

Clostridium difficile

A

Leading cause of healthcare-associated infections.

37
Q

CDC

A

morbidity and mortality weekly report publishers.

38
Q

E. coli synthesizing vitamin k and B in large intestine is an example of what type of symbiosis?

A

Mutualism.

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