Microbio 3rd Exam

Question 1

oxytrophic mutant, deficient in some metabbolic function
grow mutants on media containing the missing ingredient and media lacking it
if they can grow on media that lacks it, it means they mutated and lacks gene of interest

Answer 1

.

Question 2

cDNA, when you deal with eukaryote, the RNA is complete because introns have been removed,

Answer 2

.

Question 3

fluorescent probe, only hybridizes with complementary

differs from western blot, id specific proteins, southern for gene sequences

Answer 3

.

Question 4

7) cell engulfing prokaryotic organisms

derived mitochondria and chloroplasts

Answer 4

.

Question 5

biochemical tests are used to determine

Answer 5

enzymatic activity

Question 6

why aren’t viruses classified in a kingdom?

Answer 6

the do not have cells

Question 7

Which scientists proposed the kingdom prokaryote in 1968?

Answer 7

Murray.

Question 8

Bacteria and arachaea share which characteristic?

Answer 8

They are both prokaryotic cell types.

Question 9

Into which group would you place a single-celled photoautotroph?

Answer 9

Protist.

Question 10

Which one of the following statements describes the domain Bacteria?

Answer 10

They have prokaryotic cells

Question 11

Which domain has linear chromosomes?

Answer 11

Eukarya

Question 12

Which one of the following do not apply to eukaryotic cells?

Answer 12

Have circular chromosomes.

Question 13

Prokaryotes have 70s and Eukaryotes have 80S Ribosomes

Answer 13

.

Question 14

Treating DNA from different bacteria with the same restriction enzyme followed by separation of the fragments by electrophoresis is known as

Answer 14

DNA fingerprinting.

Question 15

Organisms that can form thin tubes called hyphae

Answer 15

Fungi

Question 16

In the scientific name staphylococcus aureus, Staphylococcus is the?

Answer 16

Genus.

Question 17

Historically, eukaryotic organisms that did not fit into other kingdoms were placed into?

Answer 17

Protista

Question 18

Cladograms for microorganisms are made from information derived from?

Answer 18

rRNA sequences.

Question 19

Fossil evidence indicates that cyanobacteria-like cells first existed on Earth how many years ago?

Answer 19

3.5 billion.

Question 20

A molecular clock for evolution is based on

Answer 20

Nucleotide sequences in the genomes of organisms.

Question 21

Which of the following is the correct order in the taxonomic hierarchy from the broadest to most specific

Answer 21

• King
• phy
• class
• order
• family
• genus
• species

Question 22

Unlike Archaea and Eukarya, the first amino acid in protein synthesis in bacteria is.

Answer 22

Formylmethionine

Question 23

Genetically related groups of eukaryotes that share a common ancestor are known as?

Answer 23

Clades.

Question 24

Which one of the following is not a major group of archaea?

Answer 24

Obligate acidophiles

Question 25

Which of the following techniques can ID antibodies in a patient's serum.

Answer 25

Western blotting.

Question 26

To which kingdom do molds belong?

Answer 26

Fungi

Question 27

The test that involves a reaction between known antibodies and an unknown bacterium is known as

Answer 27

ELISA.

Question 28

Phage typing - known virus between unknown bacterium

Answer 28

.

Question 29

A disease acquired by many people in an area in short time

Answer 29

epidemic

Question 30

In london drank broad street pump

Answer 30

john snow

Question 31

Germ-free animals

Answer 31

have increased susceptibility to disease.

Question 32

In commensalism

Answer 32

one organism benefits, the other is unaffected.

Question 33

An example of a non-communicable disease?

Answer 33

Tetanus.

Question 34

Microbial antagonism

Answer 34

preventing overgrowth of harmful microorganisms in host.

Question 35

Prodromal period

Answer 35

stage of disease that is early, mild symptoms.

Question 36

Clostridium difficile

Answer 36

Leading cause of healthcare-associated infections.

Question 37

CDC

Answer 37

morbidity and mortality weekly report publishers.

Question 38

E. coli synthesizing vitamin k and B in large intestine is an example of what type of symbiosis?

Answer 38

Mutualism.