Past Quiz & Test Questions Flashcards
formula for actual size of an object
field of view diameter (micrometres)
_____________________________________________________
# of times specimen fits across field of view
ocular lens = 10X and low power= 4X, what is the total magnification?
40X
ocular lens = 10X and medium power= 10X, what is the total magnification?
100X
ocular lens = 10X and high power= 40X, what is the total magnification?
400X
how do you find the field of view diameter?
find size of field (mm) and multiply by 1000 to convert to micrometres
what equation do we use to calculate field diameter?
HPFD LPMag.
______ = ________
LPFD HPMag.
what equation do we use to calculate field diameter on medium power?
MPFD LPMag. (40x)
______ = ________
LPFD MPMag. (100x)
HPFD 40x
______ = _____
4500 400x
find the field diameter on high power
4500 x 40
___________
400
=450 micrometres
a microscope has a LPFD of 3000μm, HPM of 400μm and LPM of 40x. Calculate the HPFD.
HPFD LPMag.
______ = ________
LPFD HPMag.
HPFD 40x
______ = ________
3000 400
40 x 3000
= __________
400
HPFD = 300μm
what is the equation to find the scale?
measured length of the drawing (μm)
________________________________________
actual size (μm)
if the measured length is 10mm, what does it become after you convert it to micrometres?
10 x 1000
=10, 000 μm
Find the scale of a drawing if the actual size of the specimen is 40 μm and measured length is 20mm.
mm–>μm = x1000
20 x 1000= 20, 000 μm
20,000 μm
______________
40
scale = 500:1 (500 times larger)
Find the field of view diameter of the high powered field of view. The low power field of view diameter is 3.5mm, the low powered objective is 4X and the high is 40X.
1) 3.5mm –> μm = 3500μm
2) 4X x 10 (ocular lens) = 40X
3) 40X x 10 (ocular lens) = 400X
4)
HPFD LPMag.
______ = ________
LPFD HPMag.
HPFD 40X
______ = _____
3500 400X
3500 x 40 / 400
HPFD =350μm
This specimen is on high power. The HPFD is 350μm, high power objective lens is 400X, fits across 6 times and FOV on low power is 3500μm.
Use only the information you need to calculate the actual size of the specimen.
of times specimen fits across
actual size
FOV diameter (μm) \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ # of times specimen fits across
350μm / 6 (times)
= 58 μm
Your lab group had trouble finding a specimen while using the high-powered objective of the microscope. What should you do to find the specimen again?
use the low power objective first to centre it and add or reduce light to see it better
How is an amoeba classified? Include 2 levels of classification
-eukaryote
-animal-like protist
How are paramecium classified? Include 2 levels of classification
-eukaryote
-animal-like protist
A microscope on low power, has a field of view that measures 4mm. The spores under the slide are photographed under low powered magnification of 40X. Goes across 7 times. Find the actual size of the organism.
actual size= 4000 / 7
= 571.43 μm
A microscope has a low powered field diameter of 4mm. Find the diameter of the field of view on high if the objective lens has a power of 4X on low and 40X on high.
4mm = 4000μm
4000 x 40
____________
400
= 400 μm
True or false: monocultures are successful because of genetic diversity
false
True or false: Bees affect genetic diversity of other species
true
true or false: prokaryotes have membrane bound organelles
false
true or false: there are 3 races of Homo sapiens sapiens
false
true or false: archaebacteria have peptidoglycan in their cell walls
false