Protein Synthesis Flashcards Preview

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Flashcards in Protein Synthesis Deck (23):
1

What are the 5 steps of protein synthesis?

Activation. Initiation. Elongation. Termination. Posttraslational modifications.

2

What is the structure of tRNA?

Cloverleaf: 3' end of molecule (CCA sequence), The T psi C loop, D loop, anticodon loop

3

In what loop does the tRNA bind to the ribosome?

T psi C loop

4

Where is the amino acid binding site on tRNA?

3' end of the molecule

5

Isoacceptor tRNA

Recognize different codons, but all have the same animo acid

6

Amino acyl tRNA synthetases

Couple the amino acid to tRNA via an ester bond. Requires 2 ATP

7

tRNA f met

Initiation codon for prokaryotes

8

What initiation factors bind to 30S subunit?

IF 1 and 3

9

What does IF2 bind to in prokaryotes?

tRNA f met (then binds to AUG at P site)

10

What is the initiation factor essential for lining up the start codon on the ribosome?

eIF-4E (is an mRNA cap binding factor)

11

What is the leader sequence for prokaryotes?

Shine-Dalgarno

12

What determines the efficiency of eukaryotic message translation?

Kozac Consensus Sequence

13

3 stages of elongation

Binding, peptide bond formation, translocation

14

Polycistronic mRNA

One mRNA can code for several proteins, and each one might be translated

15

Polyribosome

Ribosomes + mRNA during translation

16

What does the phosphorylation of eIF-2 do in eukaryotes?

Inhibits translation

17

Cycloheximide

Inhibits peptide bond formation in eukaryotic cells

18

Diphtheria toxin

Catalyzes the reaction of NAD+ w/ eukaryotic elongation factor, resulting in inhibition of translocation

19

Cloramphenicol

Inhibits peptide bond formation, and can inhibit mitochondrial protein synthesis at high levels in both eukaryotes and prokaryotes

20

Puromycin

Causes premature termination in both eukaryotes and prokaryotes

21

Tetracyline

Inhibits elongation in prokaryotes

22

Sreptomycin

Blocks translation in prokaryotes

23

Erythromycin

Inhibits translocation in prokaryotes