Special stains and cultures Flashcards Preview

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Flashcards in Special stains and cultures Deck (47):
1

Silver stain

H. pylori, Pneumocystis, Legionella

2

Tellurite agar

C. diphtheria

2

M. TB

Lowenstein-Jensen agar

2

Green metallic sheen on MacConkey agar

E. coli

4

Cryptococcus india ink prep

5

Fungi

Sabouraud agar

6

Partially acid fast oocysts of cryptosporidium 

(diarrhea in AIDS)

6

Thayer-Martin media

Neisseria spp

 

 

7

Bordetella pertussis

Bordet-Gengou (potato) agar

8

What does Thayer-Martin media contain and why is it used?

Vancomycin (kills G+), polymyxin (kills G- except Neisseria), nystatin (kills fungi)

 

Isolate N. gonorrhae or meningitidis

9

Neisseria spp

Thayer-Martin media

10

MacConkey agar

Lactose-fermenting enterics turn pink

 

(E. coli, Klebsiella)

11

Legionella silver stain (Gram - intracellular coccobacillus)

11

Needs NAD+ and hematin

H. flu

 

(Factors V and X, respectively)

11

Lactose-fermenting on MacConkey agar

E. coli, Klebsiella

12

Sabouraud agar

Fungi

13

C. diphtheria

Tellurite agar, Loeffler medium

13

Lowenstein-Jensen agar

M. TB

14

Loeffer medium

C. diphtheria

16

TB acid fast

 

(nubs = TB; long = Nocardia)

17

P. falciparum Giemsa stain

17

Requires cysteine and iron

Legionella

 

(provided on charcoal yeast extract)

19

Borrelia Giemsa

 

(spirochete! Lyme disease)

21

What does PAS stain?

Glycogen and mucopolysaccharides

22

Cryptococcus

India ink prep

23

H. pylori, Pneumocystis, Legionella

Silver stain

24

Chlamydia Giemsa stain

 

(Intranuclear inclusions)

24

H. flu

Chocolate agar, contains factor V (NAD+) and X (hematin)

26

Bordet-Gengou (potato) agar

Bordetella pertussis

28

What is the one important use of PAS?

Whipple's disease

 

(diarrhea, abdominal pain, joint pain)

29

Eaton agar

Mycoplasma pneumoniae

 

(Contains cholesterol)

31

Nocardia acid fast

 

(long = Nocardia; nubs = mycobacterium)

33

Trypansomiasis Giemsa

34

Pneumocystis silver stain

 

(Flattened footballs)

35

Needs cholesterol

Mycoplasma pneumoniae

36

Ziehl-Neelsen

Acid fast stain

 

Mycobacterium, Nocardia, oocysts of Cryptosporidium

37

T. whippeli PAS

 

(Large reddish blobs in GI mucosa)

38

Chocolate agar

H. flu

38

Mycoplasma pneumoniae

Eaton agar (contains cholesterol)

39

Non-lactose fermenting on MacConkey agar

Pseudomonas, Shigella, Proteus

40

H. pylori silver stain

 

(notice GI mucosa)

42

Charcoal yeast extract agar

Legionella

 

(Buffered with cysteine and iron)

43

Legionella

Charcoal yeast extract

 

(Contains cystiene and iron)

44

Gram - enterics

MacConkey agar to know if they're lactose-fermenting (E. coli, Klebsiella) or non-lactose-fermenting (Proteus, Pseudomonas, Shigella)

45

Giemsa stain

Chlamydia, Rickettsia, Borrelia, Plasmodium, Trypanosomes

46

India ink

Cryptococcus

47

Rickettsia Giemsa stain

 

(Morulae of Erlichiosis)