MOLECULAR MEDICINE I

Question 1

enzymes derived from bacteria
highly specific
recognise and cleave DNA at specific sequences

Answer 1

restriction endonucleases

Question 2

endonuclease may have a symmetric cut with ___ ends

endonuclease may have a asymmetric cut with ___ overhang

endonuclease may have an asymmetric cut with ___ overhang

Answer 2

blunt ends
5’ overhang
3’ overhang

Question 3

use of restriction endonucleases give rise to procedures such as

1. _____
2. _____
3. _____

Answer 3

DNA fingerprinting
formation of recombinant DNA
DNA propagating

Question 4

commonly used to separate and analyze the nucleic acids based on their size

Answer 4

gel electrophoresis

Question 5

_______: recombinant DNA replicating within host cell

______________: In-vitro DNA amplication

Answer 5

cell-based
polymerase chain reaction

Question 6

_________ is required to clone either cDNAs or copies of genes

Answer 6

vector

Question 7

3 classes of vectors

P, L, Y

Answer 7

plasmids
lambda
yeast artificial chromosome (YAC) vector

Question 8

circular DNAs found in bacteria

autonomous replication separate from the host genome

Answer 8

plasmid

Question 9

STEPS IN CELL BASED DNA PROPAGATION (CLONING)

(4 STEPS)
R-T-S-P

Answer 9

1. recombinant DNA (rDNA) production
2. transformation (introduce the rDNA to the host microorganism)
3. selection (select the microorganism that has been fully transformed)
4. propagation and purification

Question 10

DNA PROPAGATION

________: recombinant DNA replicating within host cell
________: in-vitro DNA amplification

Answer 10

cell based
polymerase chain reaction

Question 11

method of amplifying a target sequence of DNA up to 600bp

requires DNA polymerase enzyme to remain stable at high temperature

Answer 11

polymerase chain reaction

Question 12

3 steps of PCR

DD, DP, PE

Answer 12

dna denaturation
dna primer annealing
primer extension by Taq polymerase

Question 13

_____________ gene expansion can be detected by PCR

Answer 13

Huntingtin gene