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AABMicro2.7.24 Flashcards

1
Q

TSA w/sheep blood, diff w/hemolysis, most nonfastidious bacteria

A

SBA Sheep blood agar

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2
Q

Isolation of Haemophilus and Neisseria, factors X &V, incubated at increased CO2But best for…Neisseria gonnerhea better on…

A

CHOC, chocolate agarBetter for HaemophilusBest for gonnerhea and meningitis is modified thayer martin

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3
Q

CHOC supplies which factors and is incubated with increased what?

A

X, V, increased CO2

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4
Q

colistin-nalidixic acid agar, CNAisolates…suppresses…

A

isolates GPsuppresses GN

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5
Q

PEAisolates for … and …inhibits

A

isolates GPC and anaerobic GNRinhibits enteric GNR

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6
Q

PEA

A

Phenyl ethyl alcohol agar

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7
Q

CNA

A

colistin-nalidixic acid agar

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8
Q

EMB

A

eosin methylene blue

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9
Q

EMB, isolates…inhibits….LF are … To … E.coli what kind of color…

A

isolates enteric GNRinhibits GPLF are green-black to purpleE.coli have a green metallic sheen

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10
Q

MACisolates…inhibits…LF color…

A

isolates enteric GNRinhibits GP

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11
Q

bile salts and crystal violet that inhibit most GP are in what medium

A

MAC

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12
Q

SMACsorbitol MacConkey isolates……doesn’t ferment sorbitol and are colorless

A

E.coli 0157:H7

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13
Q

4 medias for isolation of salmonella and shigella

A

SS, HE, XLD, GN

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14
Q

Hektoen enteric (HE) isolates these twoinhibits

A

Salmonella and shigellainhibits normal GI flora

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15
Q

XLD

A

xylose lysine deoxycholate

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16
Q

Cold loving, optimum temp 15C

A

psychrophiles

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17
Q

moderate temp, 37C most pathogenic organisms

A

mesophiles

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18
Q

heat loving, 50-60C

A

thermophiles

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19
Q

obligate anaerobes are harmed by

A

O2

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20
Q

Facultative anaerobes can grow…

A

with or w/out O2

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21
Q

microaerophiles prefer

A

lower O2

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22
Q

capnophiles prefer

A

higher CO2

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23
Q

Aerotolerant

A

do not need O2, and not hurt by it

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24
Q

Typical incubation CO2% temp/name of ideal temphumidity

A

5-10% CO235-37C/98.6F/optiumum50-70% humidity

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25
Q

Autoclave lbs, mins, temp

A

15lbs, 15min, 121C

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26
Q

QC for wet sterilization/autoclave

A

Bacillusstearothermophilus

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27
Q

Two halogens that are disinfectants

A

idoine and chlorine

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28
Q

Process that inactivates or inhibits microbes (not spores)and example

A

disinfectionBleach (1:10)

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29
Q

Process that kills all microbes including sporesand examples

A

sterilizationautoclaves

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30
Q

Penicillin

A

beta lactaminhibits cell wells

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31
Q

Gram neg anaerobe resistant to peniciilin

A

B.fragilis

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32
Q

Two antibiotics best indicators for poor disk storage

A

penicilin and methicillin

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33
Q

Vancomycin is drug of choice for these two microbes

A

C.difficile and MRSA

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34
Q

Vancomycin is a

A

glycopoeptide

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35
Q

Kirby-Bauer Method uses what agar

A

MHMueller-Hinton

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36
Q

McFarland is what concentration and how many organisms

A

0.510*8

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37
Q

MIC

A

minimum inhibitory concentrationlowest concentration that prevents growthfirst dilution w/no growth

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38
Q

MBC

A

minimum bacteristatic concentrationlowest concentration results in >99.9% killing

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39
Q

E-test

A

MIC on a stick

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40
Q

Modified Thayer-Martin good for these two

A

N.gonorrhea and N.meningitis

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41
Q

Thayer Martin media is what kind of agar

A

enriched chocolate

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42
Q

Difference between TM and Modified and what does modified inhibit

A

MTM has trimethroprim lactate which inhibits swarming of Proteus

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43
Q

Middlebrook 7H10 and Lowenstein-Jenson good forWhich one best for observing microscopically

A

MycobacteriumMiddlebrook 7H11

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44
Q

Species for QC of dry heat oven

A

B.subtilis

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45
Q

BBE good for

A

B.fragilisBacteroides Bile Esculin

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46
Q

CCFA Cycloserine-Cefoxitinfor

A

C.difficile

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47
Q

Regan-Lowe andBordet-Gengou for…which one preferred…

A

B.pertussisRegan-Lowe preferred

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48
Q

BCYEbuffered charcoal yeast extract for

A

Legionella

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49
Q

Cystine-Tellurite, Tinsdale and Loefflers Medium

A

C.diphtheriae

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50
Q

Never refridgerate these 3

A

CSF, anaerobic and GC

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51
Q

Blood culture preped with…draw before…dilution/ml

A

prep with alcohol, iodinedraw before fever spike1:10 dilution, 10mL

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52
Q

2 Stains for acid fast/mycobacteriabest of the two…

A

Kinyoun and Ziehl-NielsonKinyoun

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53
Q

Calcofluor white stains…KOH is used to…

A

stains yeast/fungiKOH breaks down debris and mucous

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54
Q

Gram stainprimary stain, decolorizer, mordant and counterstain

A

Crystal violetacohol/acetoneiodinesafranin

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55
Q

JEMBEC plates used to

A

transport and grow N.gonorrhoeae

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56
Q

Modified Thayer-Martin, Martin LewisNYC, GC-LECT,JEMBEC isolate

A

N.gonorrhoeae and meningitidis

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57
Q

Two species of enterobacteriaceae that are nonmotile

A

shigella and klebsiella

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58
Q

Species of enterobacteriaceae that is both motile and nonmotile at diff temps

A

Yersinia motile at 25/RT, not at 35

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59
Q

Two genuses that form spore

A

Bacillus and clostridium

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60
Q

Disinfectant for walls/floors that doesn’t kill spores/myco/noneveloped

A

quarternary ammoniums

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61
Q

Phenol

A

disinfectant, doesn’t kill spores

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62
Q

2% aqueous glutaraldehyde

A

used when items can’t be sterlized by heatcold sterlization

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63
Q

Sodium polyanethod sulfonate (SPS)

A

used to grow bacteria by preventing clotting, phagocytosis and bactericial effects

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64
Q

Urethral contamination of urine is greather than Squamous epis/hpf

A

10

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65
Q

Resistance to penicillin should be tested for

A

beta lactamase production

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66
Q

beta-lactamase test that uses nitrocefin and there is hydrolysis of the beta lactim ring resulting in red color

A

Chromogenic cephalosporin metodor Nitrocefin test

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67
Q

Tests beta lactamase w/penicillin-pheno red substrate leading to color change from red to yellow to penicilloic acid

A

Acidimetric method

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68
Q

Test for beta lactamase where betalactamse hydroyze penicillin G and reduce iodine, resulting in fading of blue to colorless

A

Iodometric method

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69
Q

Potassium tellurite used to id

A

Corynebacterium diphtheriae

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70
Q

Potassium tellurite inhibits…color of C.diph

A

gp/gn nornal floragray black

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71
Q

Loeffler serum agar enhances these two things for C.diph

A

pleomorphism and metachromatic granules

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72
Q

Elek test detects toxin for

A

C.diph

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73
Q

TCBSthiosulfate citrate bile salts used for

A

Vibrio

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74
Q

2 kinds of alpha heme

A

s.pneumoviridians

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75
Q

3 kinds of beta heme

A

Group A, BL.mono

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76
Q

1 gamma heme

A

enterococci

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77
Q

GPC resistant to bacitracin

A

Staph

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78
Q

Cause of toxic shock and food poisoning

A

S.aureus

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79
Q

Staph that is nonheme and common cause of hospital aquired UTI

A

S.epi

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80
Q

staph that is mostly novobiocin sensitive

A

CNS

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81
Q

Antibiotic disk that diff between CNS and S.saproS.Sapro versus CNS susceptibility

A

NovobiocinAll CNS suspectible to Novobiocinexcept S.sapro is resistant

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82
Q

Strain of CNS that is resistant to novobiocin

A

S.sapro

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83
Q

Pigmented, high domed large GPC

A

micrococcus

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84
Q

Test that diff staph versus strep

A

catalase

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85
Q

Mannitol salt agar, MSA selects for

A

S.aureus, pos

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86
Q

90% of strep, cause of rheumatic fever, scarlet fever

A

Group A StrepS.pyogenes

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87
Q

Strep that is sensitive to BacitracinHow many units on disks

A

Group A strepS.pyogenes0.02-0.04

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88
Q

Strep NF of female genital and cause of neonatal issues

A

Group B strepS.agalactiaae

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89
Q

Diff Group A from B with what antibiotic…which one is which

A

BacitracinGroup A is sensitiveGroup B is resistant

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90
Q

Strep that is hippurate poss and CAMP pos (shows arrowhead)

A

group B strepS.agalactiae

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91
Q

beta Strep that is PYR pos

A

Grp A strep

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92
Q

Non heme strep that causes nosocomial UTI and hydrolyzes esculin

A

Group D Strepnonenterococci

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93
Q

two examples of Group D strep

A

S.bovis, S.gallolyticus

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94
Q

Used to be called strep, alpha/non heme, hydrolyzes esculin and grows in NaCL

A

enteroccouccus

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95
Q

Two oval GPC organisms that can cause nosocomial UTI, is in GI, and hydrolyze esculin

A

Group D and enterocccocci

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96
Q

What diff Group D and enterococci

A

Enterococcus grows in NaCl

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97
Q

GPC lancet shaped, alpha heme, encapsulated normal in uppper respiratory

A

S.pneumo

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98
Q

Strep that is sensitive to Optochin

A

S.pneumo

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99
Q

P disk is called

A

Optochin

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100
Q

Strep that is sensitive to optochin and is bile soluble

A

S.pneumo

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101
Q

2 Tests that diff S.pneumo from viridiansWhich one is pos for both

A

optochin and bile solubilityS.pneumo posviridians neg

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102
Q

Group F

A

microaerophillicS.milleri

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103
Q

GPC that has butterscotch odor

A

microaerophillics.milleri

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104
Q

Large GPC that are nonpatho, in environment/skin, pigmented and microdase pos

A

micrococcus

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105
Q

Antibiotic that will kill MRSA

A

Vancomycin

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106
Q

Two genera with GN dipplococci and oxidase pos

A

Neisseria, Moraxella

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107
Q

GNDC coffee bean shaped, oxidase pos,

A

Neisseria

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108
Q

GNDC that ferments glucose only

A

N.gonn

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109
Q

GNDC that is uppe respir, ferments both glucose and maltose

A

N.meningitidis

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110
Q

GNDC of upper respiratory in kids, ferments all glucose, maltose and lactose

A

N.lactamica

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111
Q

Organism that causes upper respiratory, otitis media, hockey puck colonies

A

M.cattarhalis

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112
Q

GNDC that is neg for sugars, DNase pos, butyrate esterase pos

A

M.catarrhalis

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113
Q

2 oxidase pos GNDC genera

A

N.mening,gonnM.catt

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114
Q

GNDC needs Co2, 48hrs to grow, ferments glucose only

A

N.gonn

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115
Q

N.mening transmitted by…seen mostly in…ferments these two sugars…

A

respiratory dropskidsglucose, maltose

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116
Q

These two test diff M.cat from Neisseria

A

DNase posbutyrate esterase pos

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117
Q

M.cat seen in…

A

resp infections

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118
Q

spore forming GP rods

A

bacillus

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119
Q

4 nonspore forming GP rods

A

CLENCorynebacteriumListeriaErysipelothrixNocardia

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120
Q

CLEN acronym for nonspore forming GP rods

A

CorynebacteriumListeriaErysipelothrixNocardia

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121
Q

Gram variable/pos with clue cells, bacterial vaginosis

A

Gardnerella vaginalis

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122
Q

GPR that appears as chinese letters and has metachromatic granules

A

Corynebaceriumdiphtheriae

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123
Q

Which medium will help enhance metachromic granules in C.diphtheriae

A

Loefflers

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124
Q

Which GPR has metachromatic granules on Loefflers

A

C. diphtheriae

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125
Q

Elek test tests for…in what organism

A

toxin productionC.diphtheriae

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126
Q

Ingrient in Tinsdale agar and color change due to its hydrolysisorganism diff on this

A

telluriteblackC.diphtheriae

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127
Q

GPR with tumbling motility/umbrella motility at RT

A

L.monocytogenes

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128
Q

GPR with test tube brush growth in gelatin and cause of occupational infections for fisherman etc

A

Erysipelothrixrhuiopathiae

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129
Q

GPR spores, ground glass

A

Bacillus

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130
Q

Bamboo shoots, medusa head

A

B.anthracis

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131
Q

Bacillus that causes food poisoning due to fried rice toxin

A

B.cereus

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132
Q

Most Bacillus are motile except for

A

B.anthracis

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133
Q

GPR on skin/mucous, club shaped

A

Corynebacterium

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134
Q

GPR grows on Loeffler, has metachromic granules, has TOX gene

A

Corynebacteriumdiphtheriae

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135
Q

GPR that is hospital acquired, most commonly isolated diphtheroid

A

C.jeikeium

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136
Q

GPR that can cause issues in newborns and food poisoning

A

L.mono

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137
Q

GPR causing skin/lung infections and has sulfur granules

A

Nocardia

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138
Q

Nocardia that causes skin infectionsq

A

N.brasiliensis

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139
Q

Nocardia that causes lung infections

A

N.asteroides

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140
Q

What diff L.mono fron diptheroids/corynebacterium

A

motility

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141
Q

Another name for metachromatic granules

A

Babes-Ernst granules

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142
Q

Potassium tellurite inhibits…

A

normal flora so that C..diph can grow

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143
Q

GP to Gvariable cocco bacillius that can be grown in cold enrichment

A

L.mono

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144
Q

Diptheroid that is NF in throat

A

C.pseudo-diphtheriticum

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145
Q

Two tests to diff L.mono from Corynebacteriumwhich one pos/neg

A

motility and salicinL.mono: motile, salicin +Cory: nonmotile, salicin -

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146
Q

What stain does mycobacteria use

A

acid fast, Kinyoun

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147
Q

NALC and NaOH do what for mycobacteria

A

NALC liquefyNaOH digest/decontaminate

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148
Q

Mycobacteria need to be incubated with…at…

A

with 5% CO2, at 35C

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149
Q

Two mediums for mycobacteria, which one clear and can examine microscopically

A

Middlebrook 7H11: clear plateLowenstein-Jensen: green slant

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150
Q

Tween 80 test diff and is pos inpos turns…neg is…

A

Myco kansasiipink/redamber

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151
Q

What can distinguish M.tb from others

A

Nitrate and niacin

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152
Q

Niacin and nitrate can differentiate these two mycobacteria

A

M.tbM.bovis

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153
Q

M.tb is nicacin/nitate…M.bovis is niacin/nitrate…

A

tb is posbovis is neg

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154
Q

Arylsulfatase test diff what genusArylsulfatase is an enzyme that splits free phenolphthalein from the tripotassium salt of phenolphthalein disulfite. The test for the identification of …. species is performed in a tube containing a substrate of phenolphthalein.

A

mycobacterium

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155
Q

3 stains for acid fast

A

KinyounZiehl-NeelsenFluorochrome

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156
Q

Primary stain, decolorizer, and counterstain in Kinyoun and Ziehl-Neelsen stains

A

Primary: carbolfuchsinDecolorizer: acid alcoholCounter: Methylene blue

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157
Q

Primary stain, decolorizer, and counterstain in Fluorochrome stain for acid fast

A

Primary: Auramine-rhodaminedecolorizer: acid alcoholCounter: potassium permanganate

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158
Q

Color of AFB on Kinyoun and Ziehl-Neelsen

A

red rods, blue backgroud

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159
Q

Which acid fast stain requires heat

A

Ziehl-Neelson

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160
Q

Which acid fast stain requires cold

A

Kinyoun

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161
Q

Diagnostic test and source for M.leprae

A

acid fast tissue

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162
Q

Hansens disease is also called

A

Leprosy

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163
Q

Macrophages w/acid fast bacilli

A

lepra cells

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164
Q

Lumpy jaw cause

A

A.israelii

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165
Q

Actinomyces israeli is ana or aero

A

anaerobic

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166
Q

GNR not NF, swimmers ear, Motile, grape like odorOxidase pos, cat pos

A

Pseudomonas aeruginosa

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167
Q

GNR bacterial diarrhea, seagulls, darting corkskrew motility

A

Campybacterjejuni

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168
Q

GNR ulcers, urea breath test

A

Helicobacter pylori

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169
Q

GNR TCBS, rice water stools, motile, comma shaped

A

Vibrio cholerae

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170
Q

GNCB upper respiratory, CHOC, can have capsules

A

H.influenze

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171
Q

Haemophilus pink eye

A

aegyptius

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172
Q

Haemophilus STD,chancroid, bipolar, schools of fish

A

ducreyi

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173
Q

Haemophilus that require both X and V

A

influenze, aegyptius

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174
Q

Haemophilus that requires only X (hemin)

A

ducreyi

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175
Q

H.parahaemolyticus and parainfluenzae requires which factor

A

V

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176
Q

GNR cause of trench fever, cat scratch disease

A

Bartonella

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177
Q

GNCB obligate intracellular parasiteinhalation; with undulant fever, unpasterized milk, animals, bioterrism

A

Brucella

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178
Q

GNCB tularemia/rabbit fever, level 3 pathogen

A

Francisella

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179
Q

GNR from air conditioners, Pontiact fever, BCYE (needs cytsteine)

A

Legionella

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180
Q

GNCB Pasterurella that is caused by bite/scratch, musty odor

A

multocida

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181
Q

Legionella that is brown on Feeley-Gorman iron cysteine agar

A

L.pneumophila

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182
Q

String test, positive result looks like

A

If the result is positive, the bacterial cells will be lysed by the sodium deoxycholate or Sodium taurocholate, the suspension will lose turbidity, and DNA will be released from the lysed cells causing the mixture to become viscous. A mucoid “string” is formed when an inoculating loop is drawn slowly away from the suspension.

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183
Q

String test diff these three genuses and also diff these two species

A

Vibrio spp. from Aeromonas spp. and Plesiomonas shigelloides.Vibrio cholerae (string test positive) from other Vibrio spp. (string test negative).

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184
Q

Vibrio that is yellow on TCBS, string test pos

A

Vibrio cholerae

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185
Q

Vibrios that are green on TCBS and cause bacteremia

A

parahemolyticus, vulnificus

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186
Q

The only Brucella that doesn’t grow on basic fuchin, grows on thionin

A

suis (pigs)

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187
Q

The only Brucella that needs CO2, also thionin neg

A

abortus (cow)

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188
Q

The only Brucella that doesn’t produce H2S

A

melentensis (goat)

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189
Q

Bordetella that is urease positive, oxidase positive

A

bronchiseptica

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190
Q

GN bipolar, plague, fleas/rats, safety pin

A

Yersenia pestis

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191
Q

HACEK all HACEK members are fastidious Gram-negative bacteria associated with infective endocarditis.Most HACEK are capnophilic, mouth flora, pathogenic in immunocompromised

A

HaemophilusActinobacillus/AggregatibacterCardiobacterium hominisEikenella corodensKingella kingae

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192
Q

Campylobacter is best in what atmospheric condition

A

obligate microaerophilliclikes chickens, 42C

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193
Q

obligate microaerophilliccurved GN rodgull wingedlikes monstly N

A

Campy

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194
Q

Campy gas mixture %O, CO2, N

A

85% N210% CO25% O2

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195
Q

curved GNR nonferment, microaerophillic, nitrate pos

A

C.jejuni

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196
Q

How to stain myco-plasma and urea-plasma

A

Dienes stainMycoplasma colonies can be identified by observing typical fried egg colonies on culture medium. Visualization is facilitated by application of Diene’s stain directly to the agar surface. Diene’s stain is a nonspecific stain that imparts a contrasting appearance to Mycoplasma colonies on agar. It allows easy visualization of colony morphology and characteristics.

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197
Q

Two genus of bacteria that are the smallest free living and have no cell wall

A

Myco-plasma and Urea-plasma

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198
Q

Cause of primary atypical pneumonia, walking pneumonia

A

M.pneumoniae

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199
Q

Which free living microbe has fried egg appearance

A

M.pneumoniea

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200
Q

How to stain and how to visiualize T.pallidum

A

silverdarkfield

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201
Q

Cause of syphilis

A

Treponoma pallidum

202
Q

How is leptospira passed to humans

A

via animal urine

203
Q

Borrelia that causes relapsing fever

A

B.reccurentis

204
Q

Cause of Lyme diseasetransmitted by/vector

A

B.burgdorferiIxodes ticks

205
Q

Chlamydiaceae, Rickettsia, Coxiella, and Ehrlichia all are

A

obligate intracellular parasites

206
Q

Chlamydiaceae have no…in their cell walland are stained with…

A

peptidoglycanGiesma

207
Q

Chlamydia species STD

A

trachomatis

208
Q

Chlamydia with parrot fever

A

psittaci

209
Q

Chlamydia ass. w/Guillian Barre and grown on HELA cells

A

pneumoniae

210
Q

Test that uses Proteus OX antigens to detech rickettsial antibody

A

Weil Felix

211
Q

5 Rickettsiae conditions

A

Rickettsial poxRocky Mt. Spotted FeverQ and murine fever Typhus fever

212
Q

Q Fever is actually caused by this rickettsiael organism

A

Coxiella burnetti

213
Q

PRAS

A

pre reduced anaerobically sterilizedmedia

214
Q

Classic principle of anaerobic culture

A

Jar tech, palladium pelletsH2/CO2 w/waterindicators

215
Q

GPR with double zone of beta, box car, gas gangrene, food

A

Clostridium perfringens

216
Q

Clostridium that doesn’t usually have spores

A

C.perfringens

217
Q

Naegler test on egg yolk agar used for

A

C.perfringenspos/has lecithinase activity

218
Q

Molar tooth, lumpy jaw GPR anarobe

A

Actinomyces israelii

219
Q

Acceptable Anaerobic specimens

A

BF, Blood, Bile, BMasipirate, woundBiopsy, tissue

220
Q

Unacceptable anaerobic specimens

A

swabssputum, washings, gastricvoided, catheterized urinefeces

221
Q

Anaerobe that forms black, dark halos on BBE due to esculin hydrolysis

A

Bacteroides fragilis

222
Q

Ideal atmosphere for anaerobes

A

80-90% N10% CO25%H

223
Q

QC for anaerobes

A

O2 is color, white colorless no O2

224
Q

1 GPC anaerobe1GNC anaerobe

A

GPC: Pep-GNC: Veillonella

225
Q

Finegoldia magna, Peptostreptococcus, Peptoniphilus all are what kind of microbes

A

anaerobic GPC

226
Q

4 GPR anaerobic

A

ClostridiumLactobacillusActinomycesPropionibacterium

227
Q

Anaerobic GPR with molar tooth, affects brain/facw

A

Actinomyces

228
Q

2 clostridium that have oval subterminal spores, 1 that may have

A

botulinumdifficile (perfringens may hay subterminal)

229
Q

2 Clostridium with round terminal spore

A

tetani, ramosum

230
Q

Anaerobic GPR antibiotic ass. diarrhea

A

C.difficile

231
Q

Anaerobic GPR NF skin, acne

A

Propioni-bacterium

232
Q

Anaerobic GPR NF mouth/female genital, lactic acid/pH

A

Lactobacillus

233
Q

Anaerobic GNCNF upper, red flouresnce

A

Veillonella

234
Q

Anaerobic GNR lung infection, tapered, fluoresence chartreuse/green yellow

A

Fusobacterium

235
Q

Anaerobic GNR head,neck flurousence brick red

A

Prevotella

236
Q

4 nonmotile

A

Shigella, Klebsiella (Enterobacteria)B.antracis (rest bacillus are motile)Yesenia nonmotile at 35

237
Q

Tuft of flagela at polar end

A

Lophitrichous

238
Q

Uniform flagella over body

A

Petitrichous

239
Q

Amphitrichous

A

Tuft of flagella at both poles

240
Q

Have capsules: Most are respiratory

A

Crypto neoKleb pneumoS.pneumoH.influN.memin

241
Q

3 stains for metachromatic granules, best oneMAN

A

Methylene blue bestAlbertNeisser

242
Q

Forms of sterilization

A

Autoclave 121, 15, 15Filtration Boiling Fractionalization Dry heatEthylene oxide gas

243
Q

Gram stain ingredients

A

Crystal violetIodineEthyl alcohol Safranin

244
Q

Ziel/kinyoun stain ingredients

A

Carbol fushin/phenolHCl/alcoholMethylene blue

245
Q

Naso swabs used for these two

A

H.influN.mening

246
Q

Urine cultureProcess time…Loop size, multiply by…Sig CFU…

A

1hr, 24hr fridge0.001ml, x1000>100,000 sig

247
Q

Sputum stain, best sample req

A

Gram<10 epi, >25 PMN/lpf

248
Q

ChromagarOxacillin, Cefoxitin used for

A

MRSA

249
Q

Diff microccous from Strep

A

CatalaseMicrococcus cat pos

250
Q

Staph epi from s.sapro

A

Novobiocin susceptible

251
Q

Bacitracin susceptible PYR pos

A

GAS

252
Q

Oxiase positive PVNM-CH

A

PseudomonasVibrio chNeisseriaM.cattCampyHeli,Haemo

253
Q

Genital cultureTransport with these 2, AtCulture w/in, on

A

Transport modified Stuart’s or Amies charcoal at RTCulture with in 12hrs on JEMBEC

254
Q

Agar melts at, solidify at

A

Melts 100Solidify 45-50

255
Q

Four ingredients in modified TMVCN, TL

A

VancomycinColistinNystatinTrimethoprim Lactate

256
Q

Nitrocefin is a…Used to detect…Turns..

A

Chromogenic cephalosporinDetect cleavage of beta lactam ringRed

257
Q

X factorV factor

A

X Hemin V NAD

258
Q

Needs X,Vsatelitte phenomkidsmeningitis,epiglotitis

A

H.influe

259
Q

Diff L.mono from GrpB strep

A

L.mono is motile and catalase pos

260
Q

Two microbes that are CAMP and hippyrate pos

A

GrpBL.mono

261
Q

Salicin used to diff these two.. which one pos

A

L.mono from Corynebacterium L.mono salicin pos

262
Q

GPR partially acid fast, beaded, branching, filamentous. Urease positive

A

Nocardia

263
Q

Curved Gram negative rod, sea-gull winged MicroaerophillicNeeds increased Nitrogen42C

A

Campylobacter

264
Q

Campy reqAtmosphere, temp

A

Microaerophillic 85 N10 Co25 O42C

265
Q

Aspects of enteriobacteriace

A

GNROxidase negativeCatalase posNitrate to nitriteMotile except for kleb,shig,yer Ferments glucose, acid

266
Q

Selective media for S&S with bromthymol blue and acid fuchsin as indicators

A

HE

267
Q

GPR, spores, nonmotileString of pearls w/penicillin, Medusa

A

B.anthracis

268
Q

Urease +PUNCH

A

ProteusUrea-plasmaNocardiaCrytoneo,CorynebacHelipylori

269
Q

Media where Mercury droplets can be seen in B.pertussis

A

Bordet gengou

270
Q

E.Coli IMViC

A

++–

271
Q

Kleb IMViC

A
  • -++
272
Q

Most common cause of UTI females

A

Ecoli

273
Q

Selects with Crystal violet, bile salts….diff with lactose

A

MAC

274
Q

Selects with eosin Y, methylene blue…diff w/sucrose and lactose

A

EMB

275
Q

Selects with bile salts, diff lactose/sucrose/salicin and has H2S indicator

A

HE

276
Q

What are clear and colorless on HE? What is clear with black dot?

A

Shigella clearsalmonella clear w/black dot

277
Q

What color are NF from the intestine on HE

A

orange/pink

278
Q

What is the H2S indicator in He, SS, and XLD

A

Sodium thiosulfate

279
Q

selects with brilliant green, biles salts and diff with lactose and H2S

A

SS

280
Q

Selects witih bile salts, diff with lactose/surcrose/xylose and H2S

A

XLD

281
Q

Two enterobact that produce H2S

A

Salmonella and proteus

282
Q

Enterobactero DNase pos and red

A

Serratia

283
Q

Deaminate…Enzymes that catalyse this reaction are called…

A

Deamination is the removal of an amino group from a molecule.deaminases.Deamination occurs in the presence of the deaminase enzyme that catalyzes the breaking of the bond that binds the amino group to the rest of the amino acid.

284
Q

Deaminase pos: acronym Rapid urease pos: acronym

A

Deaminase: PPMProteusProvidenciaMorganellaUrease: PMProteus, Morganella

285
Q

PPM are all pos/have these two enzymesProteusProvidenciaMoroganella

A

deaminase, urease

286
Q

Typhoid fever caused by

A

Salmonella typhi

287
Q

Positive nitrate test is…confirm with…

A

Pinkzinc powderif the medium turns red after the addition of the nitrate reagents, it is considered a positive result for nitrate reduction because it was reduced to nitrite. If it’s clear either nitrate is present or it reduced past nitrite. Confirm with zinc. After zinc if it turns red, it’s a true negative, nitrate didn’t reduce. If it’s clear after zinc, then nitrite was reduced further and is positive.

288
Q

TSI yellow butt

A

glucose

289
Q

Yellow slant

A

lactose or sucrose

290
Q

A/A which sugars

A

slant: lactose and/or sucrosebutt: glucose

291
Q

K/A

A

butt: glucose only

292
Q

K/K

A

none

293
Q

black butt

A

H2S

294
Q

Which test needs trytophan and is called Kovacs reagent

A

Indole

295
Q

Citrate tests for microbes that

A

use citrate for their sole source of carbon

296
Q

Kleb is citrate…

A

posIMViC –++

297
Q

E.coli is citrate

A

negIMViC++–

298
Q

Indicator that tests the ability of microbes to make glucose and drop the pH

A

methyl red

299
Q

EMB selects and diff these group of microbes

A

enterobactericae

300
Q

EMB has lactose to diff, what color is E.colicolor of NLF

A

green, LFclear

301
Q

Test that tests microbes ability to make acetone from glucose

A

Voges Proskauer

302
Q

Voges Proskauer2 pos: K/E2 Neg: E/Y

A

Pos: Kleb, EnterobacNeg: E.coli, Yer

303
Q

In PPA, phenylalanine is deaminated to…three PPA pos…PPM

A

phenylpyruvid acidProteusProvidenciaMorganella

304
Q

Enzyme that breaks down aminos to amines (alkaline) w/limited glucosefirst ph turns yellowwhen decarboxylized alkaline amine turns purple

A

DecarboxylaseDecarboxylation occurs in the presence of a decarboxylase enzyme that catalyzes the breaking of the bond that binds the carboxylic group to the rest of the amino acid.

305
Q

Aminos used in decarboxylase test

A

LysineArginineOrthinine

306
Q

Positive nitrate reduction test changes what color and inidicates…

A

positive changes red, indicates has only nitrate reductase

307
Q

Initial no color in negative nitrate reduction test indicates

A

No color may mean microbe has both or neither enzymes nitrate/nitrite reductase

308
Q

How to confirm no color reaction in nitrate reduction test….red..no color..

A

add powered zinc, will catalyze nitrate to nitritered…has neither enzymeclear…has both nitrite/nitrate

309
Q

To ferment lactose need these two enzymes

A

permease: brings into cellB-galactosidase: breaks down lactose

310
Q

What are microbes called that lack permease but have B-galactosidase

A

slow lactose fermenters

311
Q

Which test detects B-galactosidaseWhat does pos look like

A

ONPGit resembles lactosepos=yellow

312
Q

Sodium deoxycholate is used in

A

String test for vibrio choloera

313
Q

Test/medium that uses low peptone to allow visible acid production by oxidizers

A

OFoxidition fermentation medium

314
Q

With OF medium, what color is fermenters…where is the color for oxidizers…

A

acid, yellowoxidziers only on top due to needing oxygen

315
Q

What is the indicator in OF media

A

bromthymol blue

316
Q

Clostridium spores:oval/sub:round:

A

Clostridium spores:oval/subterminal: bot,diff, sometimes perfround terminal: tetani, ram

317
Q

Obligate intracellular parasite w/anthropod vectorGNCB that resemble bacteriaWeil-Felix (proteus ag)PCR lab titer

A

Rickettsia

318
Q

Coxiella Burretii

A

obligate intracellular parasiteQ feveraerosol from birth of infectedbioterrism

319
Q

Two genus that have intracellular morulae in cells/WBC and transmitted by ticks

A

Ehrlichia chaffeensis:mono/neuAnaplasma phagocytophilum:grans

320
Q

Lyme disease, bulls eye rashIxodes damminiserological

A

Borelia burgedorferi

321
Q

obligate intra parasiteSTD:parrots:PCR

A

Chlamydiatrachomatispsittaci

322
Q

D test

A

double diffusionD appearance due to flattened zone of inhibition, resistant to clindamycin

323
Q

Disk diffusion req

A

agar 4.0mmMH 150mmno more than 12 disksno CO2pH 7.2-7.4Ca/Mg

324
Q

Ingredient in McFarland

A

Barium Chloride

325
Q

K/Vi antigen

A

Capsular antigenVi is K, made by S.typhiPolysaccharide, heat labile, prevents phago

326
Q

H antigen

A

Flagellar Serotype SalmonellaProtein, heat labile

327
Q

O antigen

A

sOmaticSalmonella and ShigellaLipopolysaccharideHeat stable

328
Q

Opportunistic enterobacteriacea in reptiles, fresh water fish, H2S, indoor, motile++–

A

Edwardsiella tarda

329
Q

3 enterobac with ++–

A

P.vulgarisE.coliEdwardsiella

330
Q

3 enterobac with –++

A

Kleb pneuEnterobacterSerratia marcesens

331
Q

Enterobacteriaciae that swarms

A

Proteus

332
Q

SPEC is H2S

A

Salmonella ProteusEdwardsiellaCitro

333
Q

3 Nonmotile entero at 35C

A

ShigellaKlebYersenia

334
Q

Pseudomonas aeruginosa, Acinetobacter, Stenotrophomonas maltophilia are all

A

Nonfermenting gram negative rods

335
Q

Which one is NF Pseudomonas aeruginosa, Acinetobacter, Stenotrophomonas maltophilia

A

Acinetobacter of skin, pharynx

336
Q

Two GNR, cause gastroenteritis, doesn’t grow on TCBS, oxidase pos, motile

A

AeromonasPlesiomonas shigelloides

337
Q

Ornithine pos selects for

A

Enterobacter

338
Q

TSI:SEEK A/A

A

SerratiaEscherichaEnterobacterKleb

339
Q

K/K

A

Pseudomonas

340
Q

Fastidious, Capnophlic, GNCB Oral flora

A

HACEK

341
Q

HAECK, GNCB, pits agar, bleach odor

A

Eikinella corrodens

342
Q

3 GNR Enterobacteriaceae with IMViC –++

A

Kleb pneumoEnterobacterSerratia

343
Q

3 GNR Enterobacteriaceae with IMViC ++–

A

ProteusE.coliEdwardsella

344
Q

Kleb versus SerratiaMotility, lactose

A

Kleb nonmotile, LFSerratia motile, NLF

345
Q

Of these 3 no fermenting GNR, which one is oxidase posPseudo aeroAcinebacterStenotro

A

Pseudo

346
Q

2 Enterobacteriaceae that produce H2S

A

SalmonellaProteus

347
Q

3 Enterobacteriaceae that has deaminase/PPA pos

A

ProteusProvidenciaMorganella

348
Q

Enterobacteriaceae that is DNase pos

A

Serratia

349
Q

2 Enterobacteriaceae that are urease pos

A

Proteus, Morganella

350
Q

3 Enterobacteriaceae that are - -++/VP pos

A

KlebEnterobacter Serratia

351
Q

Test that diff GPC from GNDC, reactions

A

OxidaseGPC(staph, strep): oxidase negGNDC(neisseria): oxidase pos

352
Q

Of the species of mycobacterium which one isn’t a rapid grower

A

gordonae

353
Q

Lactose fermenting enterobacteraciea, acrynom/organisms

A

CEEK (seek w/a C)CitrobacterEnterobacterE.coliKleb

354
Q

M.gordonae is slow

A

M.gordonae is slow

355
Q

4 GPR anaerobic acrynom, names

A

Clostridium Lactobacillus Actinomyces Propionibacterium

356
Q

Only GN cocci anaerobic

A

Veillonella

357
Q

4 GPR nonspore1 GPR spore

A

CorneybacteriumListeria monoErysipelothrixNocardiaspore…Bacillus

358
Q

CampyHeliVibrio are all

A

GNR

359
Q

Hemophilus is what shape/color

A

GNCB

360
Q

Bartonella and Legionella gram stain

A

GNR

361
Q

Brucella, Franciella, and Pasterurella gram stain

A

GNCB

362
Q

Clostridium, Actinomyces,Propionibacterium,Lactobacillus all are …., gram stain

A

GPR anaaerobic

363
Q

Two organisms that cause pelvic inflammatory diseases

A

N.gonnChlamydia trachomatis

364
Q

Small, nonmotileCoccobacillusGrows in egg yolk sac

A

Rickettsia

365
Q

2 organism have Bacterial type cell wall

A

Chlamydia: no peptidoglycanRickettsia

366
Q

Naglar agar selects for

A

Clostridium

367
Q

GNR, fastidious, Air conditioner Best medium for legionella..L.pneumo forms brown pigment on…

A

BCYE: buffered charcoal yeast extract has cysteineL.pneumo: feeley gorman iron cysteine

368
Q

Bordetella that is urease, oxidase pos

A

Bronchiseptica

369
Q

4 ways to id mycobacteria and optimal atmosphere/% and temp

A

Growth ratePigmentBiochemTempCO2, 5%35C

370
Q

Rhodococcus equi is GPC and takes…to go from coccoid to rod

A

24hrs

371
Q

Blood cysteine glucose agar used for

A

Fran. tularensis

372
Q

β-Lactamases are enzymes that degrade the β-lactam ring. They can be encoded chromosomally or on extrachromosomal elements.This process of testing for enzyme by subculturing is called…

A

Plasmid coding

373
Q

Name of test where DNA is hydrolyzed and what positive looks like

A

DNaseMedium is colorless around organism

374
Q

In chromogenic cephalosporin test for betalactamase, the intact beta lactam ring is…If organism cleaves ring it turns ..

A

Clear/yellow=intactRed=cleaved

375
Q

Sign of Lyme disease at site of tick bite called

A

ECMErythema chronicum migrans

376
Q

Disease that causes raised, red, itchy rash (larva currens), often between your butt cheeks, on your thighs or on your wrists.

A

strongyloidiasis

377
Q

larva currens is also called

A

strongyloidiasis

378
Q

A red rash that usually begins on the face or neck, later spreading to the chest, trunk, arms and legs is called and caused by

A

Scarlet feverGrpA step

379
Q

Bulls eye is also called…Seen in what disease…

A

Erythema chronicum migransLyme

380
Q

Mycosel is…with..and…It is a highly selective media for the isolation of pathogenic fungi from materials having a large flora of other fungi and bacteria. It is not general purpose media for the isolation of all fungi (including molds and saprophytic yeasts).

A

Sabouraud Agar with Chloramphenicol and Cycloheximide

381
Q

Trichophyton that is urease-pos and penetrates hair shaft

A

Mentagrophytes

382
Q

Tuberculate macroconidia seen in what phase..Of what organism…

A

Mycelial phaseH.capsulatum

383
Q

Broad based budding yeast seen in… Phase of ..

A

Tissue phaseBlasto dermatitis

384
Q

Alternating barrel shaped arthroconidia seen in this phase… Of…

A

MycelialC.immitis

385
Q

Round, thick walled spherule with endospores seen is what phase and organism

A

YeastC.immitis

386
Q

Mariners wheel buds seen in what phase and organism

A

YeastPara brasiliensis

387
Q

Cutaneous larva migrans, creeping eruption caused by

A

Ancyclostoma braziliense

388
Q

Creeping eruption called… By….

A

Cutaneous larva migransAncyclostoma braziliense

389
Q

Capillariasis

A

The nematode (roundworm) Capillaria (=Paracapillaria) philippinensis causes human intestinal capillariasis. Unlike C. hepatica, humans are most likely the main definitive host. Transmission occurs primarily through eating undercooked fish.

390
Q

eosinophilic meningitis, eosinophilic meningoencephalitis, or EM.

A

A. cantonensis: People can get infected by eating raw or undercooked snails or slugs or contaminated produce.B. procyonis: People get infected by accidentally ingesting infectious parasite eggs. These eggs can be found in raccoon feces and environments (such as dirt) contaminated with raccoon feces.G. spinigerum: People can get infected by eating raw or undercooked freshwater fish or eels, frogs, poultry, or snakes

391
Q

In hookworm and strongly they infect by … … With what form

A

Skin penetrationFilariform

392
Q

best way to id mycoplasma

A

inhibition of growth by specific antiseraThe growth inhibition test stems from the fact that high titre antiserum added into mycoplasma growth medium will inhibit the growth of the homologous mycoplasma species against which the antiserum was produced.A method of mycoplasma species identification is described, based upon inhibition of the PPLO growth on solid media around antiserum-impregnated filter paper disks.

393
Q

Name for bacteria that ferment lactose into acid, turn media pink

A

Coliform

394
Q

CLEN versus CLAP

A

CLEN: GPR Corney, Listo, Eryt, NocardiaCLAP: GPR anaerobic Clostridium, Lacto, Acinto, Propio

395
Q

phase where growth ceases due to nutrients exhausting and toxic metabolites build up

A

stationary

396
Q

phase with litte/no multiplication but active enzymes, still maturing

A

lag

397
Q

phase where theres a logarithmic, doubling of growth/binary fision

A

exponential

398
Q

phase where waste builds up, nutrients depleted and cells stop metabolic functions and dye

A

death, declining

399
Q

Most effective form of sterilization, high pressure, high temp

A

autoclave 15lbs, 15min, 121C

400
Q

sterlization that uses moist heat-fractional alternate heating,heat, sit, heat, sit

A

fractionalization

401
Q

sterlization for proteineous things that can’t be heated

A

filtration

402
Q

sterlization at 100C, 15-30min, doesn’t kill all spores viruses

A

boiling

403
Q

Dry heat/flame and ethylene oxide gas are two forms of

A

sterlization

404
Q

process that removes, kills, deactivates all life

A

sterilization

405
Q

Dry versus moist QC species,Both are genus…Species…

A

BacillusDry: subtilisMoist: stearo-thermophilus

406
Q

Process that inactivates bacteria on nonliving surfaces

A

disinfectant

407
Q

Method using chemicals/antiseptics to destroy germs that cause infections on living tissues

A

antisepsis

408
Q

In acid fast, what is red, what is blue

A

red=acid fast bacteriablue=nonacid fast, background

409
Q

Part of gram stain that makes dye less soluble to help dye adhere to cell wall

A

iodine

410
Q

sodium poly-anethol sulfonateSPS used to…

A

grow bacteria

411
Q

Pathogenic alpha strep in upper respiratory

A

Strep pneumo

412
Q

SPS grows bacteria by preventing these three

A

prevents clotting, phagocytosis and bacterialcidal affect of human serum

413
Q

Most abundant NF in throat

A

alpha heme strep (virridans)

414
Q

Group A beta strep best collected with

A

throat swab

415
Q

H.influen, N.mening, B.pertusus best collect with

A

nasopharyngeal swabsupper respiratory

416
Q

Most common pathogen in throat

A

beta strepS.pyogenes

417
Q

Positive coagulase test is

A

clotting of plasmacoagulase reacts with plasma/fibrinogen

418
Q

Sensitive and faster way to test CSF for bacterial antigen

A

latex agg

419
Q

2 GPC that are catalase catalase positive

A

staph, micrococcus

420
Q

2 GPC that are catalases neg

A

Strep, entero

421
Q

Hippurate hydrolysis differentiates…Hippurate hydrolysis postive in these two…

A

Diff: Group B from Group APos: Group B and L.mono

422
Q

GPCNonhemolyticCatalase pos, coag negsusceptible to novobiocin

A

Staph epi

423
Q

GPC beta hemolyticBacitracin sensitivePYR +necrotizing fascitis

A

Grp AStrep pyogenes

424
Q

GPCbeta hemolysisHippurate+/CAMP+Neonatal meningitis/sepsis

A

GrpBStrep agalactiae

425
Q

GPCCNS that is resistant to novobiocin…CNS that is sensitive to novobiocin…

A

resist: saprosensitive: epi

426
Q

GPCCNScatalase +Novobiocin resistantUTI young woman

A

Staph sapro

427
Q

GPC and GNDC can be separated by this test

A

oxidase

428
Q

Neissera/Moraxella: GNDC, oxidase…Staph/Strep: GPC, oxidase…

A

Neissera/Moraxella: oxidase posStaph/Strep: oxidase neg

429
Q

GPC, lancet,capsulealpha hemesensitive to optochin, bile soluble

A

Strep pneumo

430
Q

3 Group D streps

A

Strep bovis, galloEnteroccocus

431
Q

All are….posof the 3 strep, which one likes NaCL

A

bile esculin posenterococcus loves salt

432
Q

Neissera morph…Heemophilis morph…

A

Neisseria is GN cocciHaemophilis is GN rod

433
Q

GNDC with adjacent flat sides what genus

A

Neisseria

434
Q

Both Thaymer martin and modified TM have these 4 things

A

Colistin, nystatin, vancoin chocolate agar

435
Q

What does Modified TM have taht TM doesn’t

A

trimethoprim lactateinhibits swarming of proteus

436
Q

Neisseria species all degrade…But can differentiate speicies with these two sugars…

A

all degrade glucosedifferentiate with maltose and lactorse

437
Q

N.gonn degrades…N.men degrades…N.lac degrades…

A

gonn: glucosemen: glucose, maltoselac: glucose, maltose, lactose

438
Q

GNDCoxidase positiveDNase,Butyrate +middle ear

A

Morexalla catarrhalis

439
Q

Corneybacterium diphtheriae morph

A

GPRnon motile

440
Q

GPRnonmotileBabesErnstgray black on telluritegrows on Loeffler

A

Corney diph

441
Q

Tellurite in K-tellurite inhibits…selects…

A

inhibits NF, gp/gnselects C.diph

442
Q

Medium that enhances pleomorphism, granules for Corney diph…Medium where corney diph is gray-black…

A

Loeffler serum againK-tellurite

443
Q

E-test…Elek test..

A

E-test: antimicrobial testElek: detect Corn diph toxin

444
Q

Gram positive aerobic, rest aerobic are GN-Cocci-Rod

A

Cocci:-staph, strep, micrococcus-entero, group D(bovis,gallo)Rod: BCLEN-Bacillus-Corny, Listeria, Erysi, Nocardia

445
Q

Gram positive anaerobic, rest are GN-Cocci:-Rods:

A

cocci: peptococcusRods: CLAP-Clostri,Lacto, Acinto,Propi

446
Q

Most common GNR anaerobic

A

Bacter fragilis

447
Q

Diphtheroids are corneybacterium that are NF in throat, which species is NF in throat

A

C.pseudo-diphtheriticum

448
Q

GPCBcoccocus to rod in 24hrs

A

R.equiequivalent with coccus and rod

449
Q

GP to gram variable CBcold/icecream, motile at 25catase +,CAMP+,hipp+

A

Listo. mono

450
Q

Two genus diff by motilty and salicin

A

Listeria and Corney

451
Q

motile and salicin pos…nonmotile and salicin neg…

A

pos: L.mononeg: Corney

452
Q

Strict anaerobes that are acid fast and need 5% CO2

A

myco-bacteriumnot myco-plasma (no cell wall)

453
Q

Middlebrook 7H11 used for…color…Lowenstein-Jensen used for..color…

A

Middle: mycobacteria, clearLJ: mycobacteria, green

454
Q

Tween 80 diff…pos..neg…

A

diff Mycobacterium kansasiipos: pink/redneg: amber

455
Q

Niacin/nitrate diff these two mycobacterium

A

M.tb from M.bovis

456
Q

Mycobaterium tb naicin/nitrate…M.bovis niacin/nitrate…

A

M.tb posMbovis neg

457
Q

Anaerobic organism that causes lumpy jaw

A

A.israelii

458
Q

Urease +PUNCH has urease in it

A

ProteusUrea-plasma, duh!NocardiaCrypto neo/CornyHeli

459
Q

GPRpart of CLEN(gram positive aerobic rod)will not grow with antibioticspartially acid fastbeaded, branching, filamentousurease, catalase pos

A

Nocardia

460
Q

GNR from nasopharengeal swab-fastidious, small-filamentious

A

H.influe

461
Q

Haemophilus genus needs…-influ needs…-para needs…-ducreyi needs…

A

enriched choc agarinflu: X,Vpara: Vducreyi:X

462
Q

Haemophilus will satellite on BAP with Staph streak because

A

Staph supplies the V and then hemolysis will suppply the X

463
Q

GNRfastidiousanimal bite

A

P.multo

464
Q

Cause of:-whopping cough…-meningitis, flu, epiglottis-Pontiac

A

-whopping cough: B.pertusis-meningitis, flu, epiglottis: H,influ-Pontiac: Legionella pneumo

465
Q

Bordetella that is urease/oxidase pos

A

bronchiseptica

466
Q

Brucella that doesn’t make H2S

A

melitensis doesn’t make H2S

467
Q

Brucella that is inhibited by thionin

A

arbortus inhibited by thionin

468
Q

GNRfastidiousbrown on feely-gorman iron cysteine

A

Legionella pneumoneeds cysteine

469
Q

BCYE used for

A

Legionella

470
Q

GNR fastidiousrabbits, bites, bioterrism

A

Franciellatularensis

471
Q

GNR fastidious in ACs

A

Legionella pneumo

472
Q

Campylobacteria is…Needs…%….%….%

A

micro-aerophillic85% N10% CO25% O (least amt)

473
Q

GNR curvedgull winged appearance, motilemicroaerophillic, needs increased N at 42Calso nitrate pos and nonfermentative but who the hell is going to remember that

A

Campy jejuni

474
Q

Campy jejuni uses what medium if enteritis

A

campy blood agar duh!

475
Q

Ass with peptic ulcer urea pos

A

Helicobacter pylori

476
Q

Epi cell with gram variable bacteria

A

clue cell

477
Q

Pleomorphilic, gram variableNF vagina, clue cells, sniff test

A

Gardenella vaginalis

478
Q

SS 4 media

A

XLDSSHEGN broth

479
Q

SS media ingredientsXLD:SS:HE:GN broth:

A

XLD: xylose, lysine, deoxycholateSS: brilliant green, lactoseHE: bromtyhmol blue, acid fuchsin-lactose/sucroseGN broth: deoxcholate, citrate

480
Q

Color of S/S on HE

A

clear, clear w/dot

481
Q

Two H2S indicators in triple sugar agar

A

ferric ammonium citrate and sulfate

482
Q

IMViC

A

Indole, methyl red, VP, citrate

483
Q

IMViC:E.coli…Kleb…

A

Ecoli: ++–Kleb:–++

484
Q

IMViC3 that are ++–3 that are –++

A

++– PEE: proteus, E.coli, Ed–++ KES: Kleb, Enter, Ser

485
Q

In PPA, phenalalaine is ….to phenylpyruvid acid

A

deaminated

486
Q

acrynon: Organisms that are PPA pos, deaminate phenylalanine

A

PPMP:proteusP:providenM:morganella

487
Q

3 aminos used in decarboxylase test that breaks down aminos to amines

A

LAOlysine, arginine, ornithine

488
Q

positive DNase is…two DNase pos genera…

A

clearing around colonyM.catt, Serr

489
Q

Nitrate reduction:-change color immediately=-no changed color imm, and clear w/zinc…-clear imm, color after zinc…

A

-change color imm=pos-clear, clear w/zinc=pos for both-clear imm, color w/zinc=neg

490
Q

Lactose fermenters needs these two enzymes to ferment lactose

A

Beta-galactosidasePermease

491
Q

Test that detects slow LF that lack permease but have Betagalastosidase

A

ONPG

492
Q

Oxidation fermentation media has … … to allow visibility of acid production by oxidizers

A

low peptone

493
Q

tubes used in OF…One…w/…one…

A

2one with oilone openOF need oxygen and will grow on top/yellow

494
Q

Most isolated nonfermenteroxidase posgreen, grape smellswimmers ear

A

Pseudomonas aeruginosa

495
Q

GN nonfermentative NF of skin

A

acinetobacter calcoa

496
Q

Nagler agar selects for

A

Clostridium perfringens

497
Q

Bacteroides fragilis is resistant to

A

penicillin

498
Q

Acronymn for anaerobic GPR/bacilli

A

CLAPC=clostridiumL=LactoA=AcintoP=Propioni

499
Q

The only GPC anaerobe…The only GNC anaerobe..

A

GPC: PeptococcusGNC: Veillonella

500
Q

Anaerobic GPR/bacilli that makes double zone of hemolysis

A

C.perfringens

Back up cards AAB (19 decks)

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