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bbquick Flashcards

1
Q

3 bacteria implicated in contamination of blood, 1 thats not3 parasites transmitted by blood

A

Yersinia, Citrobacter, Pseudomonas-these are the books answer-possibly staph/strepE.Coli has notPlasmodium, Trypanosoma(cruzi), Babesia

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2
Q

Primary resonse Ig, time appears…Secondary response Ig, time appears…% get immunizied to blood grp ag

A

Primary IgM, 7-14 daysSecondary IgG 1-2 days1-4%

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3
Q

ABO labels versus dyes colors-A,B,O,AB-A,B,D/A1B

A

Labels:-A: yellow-B: pink-O: blue-AB: black/gray/whiteDye:-A: blue-B: yellow-D/A1B: clear

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4
Q

anti -A1 made from…anti-H made from…

A

anti -A1 made from…Dolichos biflorusanti-H made from…Ulex europeus

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5
Q

Potentiators:-which one decreases incubation-which one books says increases sensitivity-shortens incubation, increases speed/sensitivity, decreases zeta, phosphate buffered saline/glycine-increases sensitivity for IAT, longer incubation-increases sensitivity, removes water increasing concentration of ab, enhances warm auto-eliminate rxns, can enhance coldwarm auto-reduce disulfide bonds, eliminates Kell

A

Potentiators:-decreasse incubation LISS-increase sensitvity albuminLISS low ionic strenght sln-shortens incubation, increases speed/sensitivity, decreases zeta, phosphate buffered saline/glycineBSA/albumin-increases sensitivity for IAT, longer incubationPEG-increases sensitivity, removes water increasing concentration of ab, enhances warm autoProteolitic enyzmes-eliminate rxns, can enhance coldwarm autoDTT, dithiothreitol-reduce disulfide bonds, eliminates Kell

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6
Q

Reaction grading…which one is caused by transfusion, some agg with sea of free rbc…which one is microscopic/cloud…4+3+2+1+MFWPNeg

A

Reaction grading…4+ solid button,clear3+ lrg clump, clear2+ med clumps, clear1+ sml agg, turbidMF some agg, sea of free(transfusion)WP microscopic cloudyNeg no agg,cloudy

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7
Q

Allogenic Donor reqs:Age:Temp/C/F:Wt:Hmg/Hct: femalemaleBP:Pulse:

A

Allogenic Donor reqs:Age: >=16Temp/C/F: 37.5C/99.5FWt: >=110lb/50kgHmg/Hct: 12.5/38%female 12.5/38%male 13/39BP: 90-180/50-100Pulse: 50-100

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8
Q

Autologous donor req…Age..Hmg/HCT..other…

A

Autologous-no age req-Hmg/HCT 11/33%-no bacterimia-collect >72hrs before-ABO/Rh testing

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9
Q

Vaccine defferments..TB skin pos…measles/mumps/rubeola/polio no symptoms..Hep B…Germen measles/chicken pox/ West Nile….

A

Vaccine defferments..TB skin pos…no defferal if all else normalmeasles/mumps/rubeola/polio no symptoms..2 wksHep B…3 wksGermen measles/chicken pox/ West Nile….4 wks

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10
Q

Wait time for plasmapheresis donor to wait before donate WB…Wait time for WB donor to donate plasmapheresis…

A

Wait time for plasmapheresis donor to wait before donate WB…48hrsWait time for WB donor to donate plasmapheresis…72hrs-need to test TP if >1x/2mths at 4mth intervals

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11
Q

Main reason to be defered for 1yrs…3 random…

A

exposure to hepatitis, malaria, HIV-had sex/living with, tatoo/trans, hep B immuneglobulin, mucous/skin->72hrs in jail-completed syphillis/gonn treatment or reactive STS-bitten by dog

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12
Q

Reason to be deffered 3yrs

A

asymptomatic from endemic area of malaria or had malaria before/completed treatment

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13
Q

Permnament deferral main reasons…random reason…

A

Permnament..-definete disease, habits high risk for hep/malaria/CJD,Babeosis,T.cruzi, HIV, HTLV-Viral hep after age 11-drug-human growth hormone

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14
Q

Main testing for products…

A

ABO/Rh, ab screen-Syphillis/RPR-Hepatitis(HBsAg,antiHBc, antiHCV)-HIV(anti1/2)-HTLV(anti1/2)-T.cruzi, WN, Zika, Babesia

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15
Q

RBC temps etcstorage…transport..open good for…Frozen RBC temp, time-% glycerol, thaw at, store at, good for1 unit raises hmg/hct by

A

RBC nonfrozen-store 1-6C-transport 1-10-open=24hrsFrozen: -65C, 10yrs-40% glycerol, thaw at 37C, store 1-6, good for 24hrsraise 1g/3%(rbcx3=hct)

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16
Q

FFP storage:-shorest time, longest time-thaw temp, store at, time-good for..Cryo storage-2 main things contain-good for once thawed, kept at-thawed/open time

A

FFP coag def-1 yr at -18-7yrs at -65-thaw at 30-37, store 1-6, 24hrsCryo fibrinogen, F8-1 yr at -18-6hrs thawed, leave at RT-4hrs thawed/pooled

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17
Q

PLTS:-days, temp, with-open: pooled, apheresisGranulocytes/washed-time good for with no-washed preventsIrradiated -days -prevents

A

PLTS-light spin removes rbc, heavy spins down wbc/plts-5 days, 20-24 w/agitation-4 hrs plts pooled open-24hrs aphresis openGranulocytes: 24hrs, 20-24,no agitationwashed rbc: 24hrs, prevents allergy to plasma proteins in IgA defIrradiated: 28 days, prevents GVHD

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18
Q

additivesshorest to longest…

A

rejuvenating sln: restores 2,3DPG/ATP-store 1-6C, use w/in 24hrsACD/CPD/CPD2: 21 days-anticoag citrate dextrose, -citrate phosphate dextroseCPDA1: 35 days, has adenine at endADSOL/AS/additives: 42 days

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19
Q

Storage of blood changes

A

Decreased:-pH (lactic acid)-Na (moves into cells while K moves out)-2-3DPGIncreased: -K(moves out while Na moves in)-NH4

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20
Q

Backbone of sugars is…O gene is…H is…-H gene codes for/what is glycosyltransferase/sugar A gene codes for..B gene codes for..

A

Backbone is Gal with Fuc off bottomGenes add onto Gal to the left…O gene is inactive, H is unmodifiedhas fuco-syl-transferase/fucoseA has N-acetyl-galac(NAC)B has D-galac

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21
Q

Bombay phenotype is..antibodies in serum..Phenotype with absense of Rh…

A

absense of ABO ag, Ohhas A,B,H-type as O-not agg w/anti-H, no HRhnull is absense of Rh ag

22
Q

H ag greatest to leastgreatest, least

A

greatest H is O, least is Oh(none)A2>B>A2B>A1>A1BO>A2>B>A2B»A1>A1B>Oh-A2>B-more A, B less H so A2>A2B-A2>A1

23
Q

Se: expression ABO ag in BF, secretor status-dominant, 80% have Se geneSe codes for these three…-need….to have A or B-Se doesn’t code forLe: adsorbed onto red cell, in saliva/plasma-need Le to make…-need.. to make…

A

Se codes for H, then A and B, doesn’t code for SeLe:-need Le to make Lea-need Lea to make Leb

24
Q

ABO frequency greatest to least/%

A

O>A>B>ABO 46A 41B 9AB 4

25
Q

Subgroups:-MF with antiA, neg with antiA1 lectin…-A1 cell pos, antiA1 lectin neg-antiA 3+, antiB 3+, A1 cells 2+, antiA1Lectin neg

A

Subgroups:A3 is mixed field w/antiA1Ax isnt mixedMF with antiA, neg with antiA1 lectin: A3-barely any A=MF,-antiA1 lectin neg=no A1 ag=not A1A1 cell pos, antiA1 lectin neg=Ax-A1 cell pos=has antiA1-antiA1 lectin neg=not A1antiA 3+, antiB 3+, A1 cells 2+, antiA1Lectin neg: A2B-antiA,anti B 3+=AB-A1 cells 2+=has antiA1=not A1-antiA1 lectin neg=not A1=A2

26
Q

Autoab that is IgG, donath landsteiner, PCHab that is IgM, cold agg disease, Mycoplasma pneumonia, blacks…ab seem infants, infectious mono…

A

P: Autoab that is IgG, donath landsteiner, PCHI: ab that is IgM, cold agg disease, Mycoplasma pneumonia, blacks…i: ab seem infants, infectious mono…

27
Q

5 naturally occuring ab

A

ABOLewis, P, MCw

28
Q

Rh null…Oh…

A

Rh null no Rh agOh no ABO ag

29
Q

Tja part of..Cw/f part of…U part of…

A

Tja/PP1PK part of PCw/f part of RhU part of MNS

30
Q

D how many have…K, k how many have…

A

D 85% haveK only 9% hask 99% has

31
Q

most little letters has 90-100% frequencys,e,k

A

s=90e=98k=99

32
Q

most others have 70-85%…except these 3….

A

70-85% -Fya/Fyb, Jka/Jkb-M/N, P1-Lebexceptions…Lea=22E=29S 55

33
Q

Fy…-Ig, temp-dosage/enzymes?-Fya-b-, seen in, %, resistance to-most common ab

A

Fy-IgG, 37/AHG-dosage/destroyed by enzymes-Fya-b- blacks, 68%, resist vivax/knowlesi-Fya ab more common

34
Q

3 groups that dont cause HDN

A

lewis, lutheranM/N

35
Q

Kidd…Jka/Jkb/Jk3Ig, temp, dosage/enzymes, seen in

A

IgG, 37/AHG, show dosage, enhanced by enzymes, seen in delayed trans rxn

36
Q

Kell: next to D, 2nd most potent ag-% lackIg, temp, enzymes, dosageTwo systems in K, which one low/highwhich one Mcleod, xlinked males, Ko genesk: cellano-how many have

A

Kell: no one has, no dosage/no enzyme-90% lack, antibody common easy to find units-IgG,37/AHG, unffected by enzymes/no dosage-Kpa/Jsa is low-Kpb/Jsb is high-Kx: 100% have, ones that don’t are McLeod,males-K0/nullk/Cellano: 99 have

37
Q

System with MF, RT/saline, no HDN destroyed by enzymes

A

Lutheran

38
Q

Grp IgM, common in all esp blacks, cold reactive, not on infant, cold agg disease, M.pneumo

A

I

39
Q

Rh..Ig, temp, dosage enzymes% have D…most common rh neg genotype…most common cause of HDN next to D…weak D common in…

A

Rh-IgG, 37/AHG, dosage/enzymes-85% have, weak D blacks-genotype: cde/cde-most common HDN after D is c

40
Q

IgM, enhanced by enzymes, naturally occuring, neutralized by hydatid cyst

A

P1

41
Q

IgG, 37/AHGpart of M/N, if you have this you have UU neg is seen in…and resistant to…

A

S/spart of M/N, have S, have UUneg blacks, resistant to ficin

42
Q

Dosage…4 grps

A

-Kidd, Fy, Rh-M/N/Skidd and duffy the rh monkey eat lots of MNS

43
Q

Enyzmes destroy…all but….are enhanced…

A

enzymes destroy-Fy, M/N/Sall but Kell are enhanced

44
Q

QCfridge, freezer..temp, how oftencheck alarm…chartstemp…waterbath/heat…centrifuge…how often

A

fridge 1-6, freezer -18 dailycharts weeklycentrifuge 6mthsalarm 3x mthtemp w/NIST yearlywaterbath/heat 37

45
Q

Polyspecific AHG has…color…how to check reagent…type of cells, rxnIf CC agg, means these 3 things

A

IgG and C3d (not b), greencheck with check cells, grpO, WPCC agg means-ahg added-washed well-ahg didnt react with PT red cells

46
Q

AHG testing..false pos..false neg…

A

false pos:-pos DAT/complement fixed-over centrifuging/reading-septicemia/bacteria -Whartons jelly in cordfalse neg:-inadequate washing: ahg neutralized, ab eluted-prozone-dilution w/saline, didn’t add reagent-over/under centrifugation/incubation

47
Q

IAT time, tempDAT temp

A

IAT 15-60min (w/out LISS) at 37, washDAT wash cells first, then add poly, IS-if neg sit RT

48
Q

3 drugs that cause false pos DAT1 drug doesn’t

A

penicillin, cephalothinmethyldopaaspirin wont

49
Q

Common Rh antigen that warm autoab directed against

A

e

50
Q

Ab screen/ID rxns…-same strength in 1 phase…-varying strength…-rxn diff phases…-all cells in AHG, AC pos…-all cells in AHG AC neg…-all cells at 37, neg AHG, AC pos….-no rxn in screen/ab panel but xmatch incompatible….-only rxn in AHG and PT anemic, AC pos….

A

Ab screen/ID rxns…-same strength in 1 phase…single ab-varying strength…multiple ab-rxn diff phases…warm, cold-all cells in AHG, AC pos…warm auto-all cells in AHG, AC neg…high frequency ag-all cells at 37, neg AHG, AC pos….rouleaux-no rxn in screen/ab panel but xmatch incompatible….low freq ab-only rxn in AHG and PT anemic, AC pos….warm auto HA

51
Q

Procedure to remove ab bound to sensitized rbc…cannot remove….Procedure to separate multiple ab…

A

elution, cannot remove complementmultiple=adsorption

52
Q

AET/DTT, ZZAP, 2ME all are….and….

A

sulfhydrl reagents, inactivate

Back up cards AAB (19 decks)

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