Microdeck_14431061 (1) Flashcards
(325 cards)
1
Q
TSA w/sheep blood, diff w/hemolysis, most nonfastidious bacteria
A
SBA Sheep blood agar
2
Q
Isolation of Haemophilus and Neisseria, factors X &V, incubated at increased CO2But best for…Neisseria gonnerhea better on…
A
CHOC, chocolate agarBetter for HaemophilusBest for gonnerhea and meningitis is modified thayer martin
3
Q
CHOC supplies which factors and is incubated with increased what?
A
X, V, increased CO2
4
Q
Columbia colistin-nalidixic acid agar, CNAisolates…suppresses…
A
isolates GPsuppresses GN
5
Q
Phenyl ethyl alcohol agarPEAisolates for … and …inhibits
A
isolates GPC and anaerobic GNRinhibits enteric GNR
6
Q
PEA
A
Phenyl ethyl alcohol agar
7
Q
Columbia colistin-nalidixic acid agar
A
CNA
8
Q
EMB
A
eosin methylene blue
9
Q
EMB, isolates…inhibits….LF are … To … E.coli what kind of color…
A
isolates enteric GNRinhibits GPLF are green-black to purpleE.coli have a green metallic sheen
10
Q
MACisolates…inhibits…LF color…
A
isolates enteric GNRinhibits GP
11
Q
bile salts and crystal violet that inhibit most GP are in what medium
A
MAC
12
Q
SMACsorbitol MacConkey isolates……doesn’t ferment sorbitol and are colorless
A
E.coli 0157:H7
13
Q
4 medias for isolation of salmonella and shigella
A
SS, HE, XLD, GN
14
Q
Hektoen enteric (HE) isolates these twoinhibits
A
Salmonella and shigellainhibits normal GI flora
15
Q
XLD
A
xylose lysine deoxycholate
16
Q
Cold loving, optimum temp 15C
A
psychrophiles
17
Q
moderate temp, 37C most pathogenic organisms
A
mesophiles
18
Q
heat loving, 50-60C
A
thermophiles
19
Q
obligate anaerobes are harmed by
A
O2
20
Q
Facultative anaerobes can grow…
A
with or w/out O2
21
Q
microaerophiles prefer
A
lower O2
22
Q
capnophiles prefer
A
higher CO2
23
Q
Aerotolerant
A
do not need O2, and not hurt by it
24
Q
Typical incubation CO2% temp/name of ideal temphumidity
A
5-10% CO235-37C/98.6F/optiumum50-70% humidity
25
Autoclave lbs, mins, temp
15lbs, 15min, 121C
26
QC for wet sterilization/autoclave
Bacillusstearothermophilus
27
Two halogens that are disinfectants
idoine and chlorine
28
Process that inactivates or inhibits microbes (not spores)and example
disinfectionBleach (1:10)
29
Process that kills all microbes including sporesand examples
sterilizationautoclaves
30
Penicillin
beta lactaminhibits cell wells
31
Gram neg anaerobe resistant to peniciilin
B.fragilis
32
Two antibiotics best indicators for poor disk storage
penicilin and methicillin
33
Vancomycin is drug of choice for these two microbes
C.difficile and MRSA
34
Vancomycin is a
glycopoeptide
35
Kirby-Bauer Method uses what agar
MHMueller-Hinton
36
McFarland is what concentration and how many organisms
0.510*8
37
MIC
minimum inhibitory concentrationlowest concentration that prevents growthfirst dilution w/no growth
38
MBC
minimum bacteristatic concentrationlowest concentration results in >99.9% killing
39
E-test
MIC on a stick
40
Modified Thayer-Martin good for these two
N.gonorrhea and N.meningitis
41
Thayer Martin media is what kind of agar
enriched chocolate
42
Difference between TM and Modified
MTM has trimethroprim lactate which inhibits swarming of Proteus
43
Middlebrook 7H10 and Lowenstein-Jenson good forWhich one best for observing microscopically
MycobacteriumMiddlebrook 7H11
44
Species for QC of dry heat oven
B.subtilis
45
BBE good for
B.fragilisBacteroides Bile Esculin
46
CCFA Cycloserine-Cefoxitinfor
C.difficile
47
Regan-Lowe andBordet-Gengou for...which one preferred...
B.pertussisRegan-Lowe preferred
48
BCYEbuffered charcoal yeast extract for
Legionella
49
Cystine-Tellurite, Tinsdale and Loefflers Medium
C.diphtheriae
50
Never refridgerate these 3
CSF, anaerobic and GC
51
Blood culture preped with...draw before...dilution/ml
prep with alcohol, iodinedraw before fever spike1:10 dilution, 10mL
52
2 Stains for acid fast/mycobacteriabest of the two...
Kinyoun and Ziehl-NielsonKinyoun
53
Calcofluor white stains...KOH is used to...
stains yeast/fungiKOH breaks down debris and mucous
54
Gram stainprimary stain, decolorizer, mordant and counterstain
Crystal violetacohol/acetoneiodinesafranin
55
JEMBEC plates used to
transport and grow N.gonorrhoeae
56
Modified Thayer-Martin, Martin LewisNYC, GC-LECT,JEMBEC isolate
N.gonorrhoeae and meningitidis
57
Two species of enterobacteriaceae that are nonmotile
shigella and klebsiella
58
Species of enterobacteriaceae that is both motile and nonmotile at diff temps
Yersinia
59
Two genuses that form spore
Bacillus and clostridium
60
Disinfectant for walls/floors that doesn't kill spores/myco/noneveloped
quarternary ammoniums
61
Phenol
disinfectant, doesn't kill spores
62
2% aqueous glutaraldehyde
used when items can't be sterlized by heatcold sterlization
63
Sodium polyanethod sulfonate (SPS)
used to grow bacteria by preventing clotting, phagocytosis and bactericial effects
64
Urethral contamination of urine is greather than Squamous epis/hpf
10
65
Resistance to penicillin should be tested for
beta lactamase production
66
beta-lactamase test that uses nitrocefin and there is hydrolysis of the beta lactim ring resulting in red color
Chromogenic cephalosporin metodor Nitrocefin test
67
Tests beta lactamase w/penicillin-pheno red substrate leading to color change from red to yellow to penicilloic acid
Acidimetric method
68
Test for beta lactamase where betalactamse hydroyze penicillin G and reduce iodine, resulting in fading of blue to colorless
Iodometric method
69
Potassium tellurite used to id
Corynebacterium diphtheriae
70
Potassium tellurite inhibits...color of C.diph
gp/gn nornal floragray black
71
Loeffler serum agar enhances these two things for C.diph
pleomorphism and metachromatic granules
72
Elek test detects toxin for
C.diph
73
TCBSthiosulfate citrate bile salts used for
Vibrio
74
2 kinds of alpha heme
s.pneumoviridians
75
3 kinds of beta heme
Group A, BL.mono
76
1 gamma heme
enterococci
77
GPC resistant to bacitracin
Staph
78
Cause of toxic shock and food poisoning
S.aureus
79
Staph that is nonheme and common cause of hospital aquired UTI
S.epi
80
staph that is mostly novobiocin sensitive
CNS
81
Antibiotic disk that diff between CNS and S.saproS.Sapro versus CNS susceptibility
NovobiocinAll CNS suspectible to Novobiocinexcept S.sapro is resistant
82
Strain of CNS that is resistant to novobiocin
S.sapro
83
Pigmented, high domed large GPC
micrococcus
84
Test that diff staph versus strep
catalase
85
Mannitol salt agar, MSA selects for
S.aureus, pos
86
90% of strep, cause of rheumatic fever, scarlet fever
Group A StrepS.pyogenes
87
Strep that is sensitive to BacitracinHow many units on disks
Group A strepS.pyogenes0.02-0.04
88
Strep NF of female genital and cause of neonatal issues
Group B strepS.agalactiaae
89
Diff Group A from B with what antibiotic...which one is which
BacitracinGroup A is sensitiveGroup B is resistant
90
Strep that is hippurate poss and CAMP pos (shows arrowhead)
group B strepS.agalactiae
91
beta Strep that is PYR pos
Grp A strep
92
Non heme strep that causes nosocomial UTI and hydrolyzes esculin
Group D Strepnonenterococci
93
two examples of Group D strep
S.bovis, S.gallolyticus
94
Used to be called strep, alpha/non heme, hydrolyzes esculin and grows in NaCL
enteroccouccus
95
Two oval GPC organisms that can cause nosocomial UTI, is in GI, and hydrolyze esculin
Group D and enterocccocci
96
What diff Group D and enterococci
Enterococcus grows in NaCl
97
GPC lancet shaped, alpha heme, encapsulated normal in uppper respiratory
S.pneumo
98
Strep that is sensitive to Optochin
S.pneumo
99
P disk is called
Optochin
100
Strep that is sensitive to optochin and is bile soluble
S.pneumo
101
2 Tests that diff S.pneumo from viridiansWhich one is pos for both
optochin and bile solubilityS.pneumo posviridians neg
102
Group F
microaerophillicS.milleri
103
GPC that has butterscotch odor
microaerophillics.milleri
104
Large GPC that are nonpatho, in environment/skin, pigmented and microdase pos
micrococcus
105
Antibiotic that will kill MRSA
Vancomycin
106
Two genera with GN dipplococci and oxidase pos
Neisseria, Moraxella
107
GNDC coffee bean shaped, oxidase pos,
Neisseria
108
GNDC that ferments glucose only
N.gonn
109
GNDC that is uppe respir, ferments both glucose and maltose
N.meningitidis
110
GNDC of upper respiratory in kids, ferments all glucose, maltose and lactose
N.lactamica
111
Organism that causes upper respiratory, otitis media, hockey puck colonies
M.cattarhalis
112
GNDC that is neg for sugars, DNase pos, butyrate esterase pos
M.catarrhalis
113
3 oxidase pos GNDC
N.mening,gonnM.catt
114
GNDC needs Co2, 48hrs to grow, ferments glucose only
N.gonn
115
N.mening transmitted by...seen mostly in...ferments these two sugars...
respiratory dropskidsglucose, maltose
116
These two test diff M.cat from Neisseria
DNase posbutyrate esterase pos
117
M.cat seen in...
resp infections
118
spore forming GP rods
bacillus
119
4 nonspore forming GP rods
CLENCorynebacteriumLiseriaErysipelothrixNocardia
120
CLEN acronym for nonspore forming GP rods
CorynebacteriumListeriaErysipelothrixNocardia
121
Gram variable/pos with clue cells, bacterial vaginosis
Gardnerella vaginalis
122
GPR that appears as chinese letters and has metachromatic granules
Corynebaceriumdiphtheriae
123
Which medium will help enhance metachromic granules in C.diphtheriae
Loefflers
124
Which GPR has metachromatic granules on Loefflers
C. diphtheriae
125
Elek test tests for...in what organism
toxin productionC.diphtheriae
126
Ingrient in Tinsdale agar and color change due to its hydrolysisorganism diff on this
telluriteblackC.diphtheriae
127
GPR with tumbling motility/umbrella motility at RT
L.monocytogenes
128
GPR with test tube brush growth in gelatin and cause of occupational infections for fisherman etc
Erysipelothrixrhuiopathiae
129
GPR spores, ground glass
Bacillus
130
Bamboo shoots, medusa head
B.anthracis
131
Bacillus that causes food poisoning due to fried rice toxin
B.cereus
132
Most Bacillus are motile except for
B.anthracis
133
GPR on skin/mucous, club shaped
Corynebacterium
134
GPR grows on Loeffler, has metachromic granules, has TOX gene
Corynebacteriumdiphtheriae
135
GPR that is hospital acquired, most commonly isolated diphtheroid
C.jeikeium
136
GPR that can cause issues in newborns and food poisoning
L.mono
137
GPR causing skin/lung infections and has sulfur granules
Nocardia
138
Nocardia that causes skin infectionsq
N.brasiliensis
139
Nocardia that causes lung infections
N.asteroides
140
What diff L.mono fron diptheroids/corynebacterium
motility
141
Another name for metachromatic granules
Babes-Ernst granules
142
Potassium tellurite inhibits...
normal flora so that C..diph can grow
143
GP to Gvariable cocco bacillius that can be grown in cold enrichment
L.mono
144
Diptheroid that is NF in throat
C.pseudo-diphtheriticum
145
Two tests to diff L.mono from Corynebacteriumwhich one pos/neg
motility and salicinL.mono: motile, salicin +Cory: nonmotile, salicin -
146
What stain does mycobacteria use
acid fast, Kinyoun
147
NALC and NaOH do what for mycobacteria
NALC liquefyNaOH digest/decontaminate
148
Mycobacteria need to be incubated with...at...
with 5% CO2, at 35C
149
Two mediums for mycobacteria, which one clear and can examine microscopically
Middlebrook 7H11: clear plateLowenstein-Jensen: green slant
150
Tween 80 test diff and is pos inpos turns...neg is...
Myco kansasiipink/redamber
151
What can distinguish M.tb from others
Nitrate and niacin
152
Niacin and nitrate can differentiate these two mycobacteria
M.tbM.bovis
153
M.tb is nicacin/nitate...M.bovis is niacin/nitrate...
tb is posbovis is neg
154
Arylsulfatase test diff what genusArylsulfatase is an enzyme that splits free phenolphthalein from the tripotassium salt of phenolphthalein disulfite. The test for the identification of .... species is performed in a tube containing a substrate of phenolphthalein.
mycobacterium
155
3 stains for acid fast
KinyounZiehl-NeelsenFluorochrome
156
Primary stain, decolorizer, and counterstain in Kinyoun and Ziehl-Neelsen stains
Primary: carbolfuchsinDecolorizer: acid alcoholCounter: Methylene blue
157
Primary stain, decolorizer, and counterstain in Fluorochrome stain for acid fast
Primary: Auramine-rhodaminedecolorizer: acid alcoholCounter: potassium permanganate
158
Color of AFB on Kinyoun and Ziehl-Neelsen
red rods, blue backgroud
159
Which acid fast stain requires heat
Ziehl-Neelson
160
Which acid fast stain requires cold
Kinyoun
161
Diagnostic test and source for M.leprae
acid fast tissue
162
Hansens disease is also called
Leprosy
163
Macrophages w/acid fast bacilli
lepra cells
164
Lumpy jaw cause
A.israelii
165
Actinomyces israeli is ana or aero
anaerobic
166
GNR not NF, swimmers ear, Motile, grape like odorOxidase pos, cat pos
Pseudomonas aeruginosa
167
GNR bacterial diarrhea, seagulls, darting corkskrew motility
Campybacterjejuni
168
GNR ulcers, urea breath test
Helicobacter pylori
169
GNR TCBS, rice water stools, motile, comma shaped
Vibrio cholerae
170
GNCB upper respiratory, CHOC, can have capsules
H.influenze
171
Haemophilus pink eye
aegyptius
172
Haemophilus STD,chancroid, bipolar, schools of fish
ducreyi
173
Haemophilus that require both X and V
influenze, aegyptius
174
Haemophilus that requires only X (hemin)
ducreyi
175
H.parahaemolyticus and parainfluenzae requires which factor
V
176
GNR cause of trench fever, cat scratch disease
Bartonella
177
GNCB obligate intracellular parasiteinhalation; with undulant fever, unpasterized milk, animals, bioterrism
Brucella
178
GNCB tularemia/rabbit fever, level 3 pathogen
Francisella
179
GNR from air conditioners, Pontiact fever, BCYE (needs cytsteine)
Legionella
180
GNCB Pasterurella that is caused by bite/scratch, musty odor
multocida
181
Legionella that is brown on Feeley-Gorman rion cysteine agar
L.pneumophila
182
String test, positive result looks like
If the result is positive, the bacterial cells will be lysed by the sodium deoxycholate or Sodium taurocholate, the suspension will lose turbidity, and DNA will be released from the lysed cells causing the mixture to become viscous. A mucoid “string” is formed when an inoculating loop is drawn slowly away from the suspension.
183
String test diff these three genuses and also diff these two species
Vibrio spp. from Aeromonas spp. and Plesiomonas shigelloides.Vibrio cholerae (string test positive) from other Vibrio spp. (string test negative).
184
Vibrio that is yellow on TCBS, string test pos
Vibrio cholerae
185
Vibrios that are green on TCBS and cause bacteremia
parahemolyticus, vulnificus
186
The only Brucella that is basic fuchin pos
suis (pigs)
187
suis is fuchin pos
Brucella that is fuchin pos
188
The only Brucella that is thionin neg
abortus (cow)
189
abortus thionin neg
Brucella that is thionin neg
190
The only Brucella that is H2S neg
melentensis (goat)
191
melentensis doesn't H2S
Brucella that doesn't producte H2S
192
Bordetella that is urease positive, oxidase positive
bronchiseptica
193
GN bipolar, plague, fleas/rats, safety pin
Yersenia pestis
194
HACEKMost HACEK are capnophilic, mouth flora, pathogenic in immunocompromised
HaemophilusActinobacillusCardiobacterium hominisEikenella corodensKingella kingae
195
Campylobacter is best in what atmospheric condition
obligate microaerophilliclikes chickens, 42C
196
obligate microaerophilliccurved GN rodgull wingedlikes monstly N
Campy
197
Campy gas mixture %O, CO2, N
5% O210% CO285% N2
198
curved GNR nonferment, microaerophillic, nitrate pos
C.jejuni
199
How to stain mycoplasma and ureaplasma
Dienes stain
200
Two genus of bacteria that are the smallest free living and have no cell wall
Mycoplasma and Ureaplasma
201
Cause of primary atypical pneumonia, walking pneumonia
M.pneumoniae
202
Which free living microbe has fried egg appearance
M.pneumoniea
203
How to stain and how to visiualize T.pallidum
silverdarkfield
204
Cause of syphilis
Treponoma pallidum
205
How is leptospira passed to humans
via animal urine
206
Borrelia that causes relapsing fever
B.reccurentis
207
Cause of Lyme diseasetransmitted by
B.burgdorferiIxodes ticks
208
Chlamydiaceae, Rickettsia, Coxiella, and Ehrlichia all are
obligate intracellular parasites
209
Chlamydiaceae have no...in their cell walland are stained with...
peptidoglycanGiesma
210
Chlamydia species STD
trachomatis
211
Chlamydia with parrot fever
psittaci
212
Chlamydia ass. w/Guillian Barre and grown on HELA cells
pneumoniae
213
Test that uses Proteus OX antigens to detech rickettsial antibody
Weil Felix
214
5 Rickettsiae conditions
Rickettsial poxRocky Mt. Spotted FeverQ and murine fever Typhus fever
215
Q Fever is actually caused by this rickettsiael organism
Coxiella burnetti
216
PRAS
pre reduced anaerobically sterilizedmedia
217
Classic principle of anaerobic culture
Jar tech, palladium pelletsH2/CO2 w/waterindicators
218
GPR with double zone of beta, box car, gas gangrene, food
Clostridium perfringens
219
Clostridium that doesn't usually have spores
C.perfringens
220
Naegler test on egg yolk agar used for
C.perfringens
221
Molar tooth, lumpy jaw GPR anarobe
Actinomyces israelii
222
Acceptable Anaerobic specimens
BF, Blood, Bile, BMasipirate, woundBiopsy, tissue
223
Unacceptable anaerobic specimens
swabssputum, washings, gastricvoided, catheterized urinefeces
224
Anaerobe that forms black, dark halos on BBE due to esculin hydrolysis
Bacteroides fragilis
225
Ideal atmosphere for anaerobes
80-90% N10% CO25%H
226
QC for anaerobes
O2 is color, white colorless no O2
227
Finegoldia magna, Peptostreptococcus, Peptoniphilus all are what kind of microbes
anaerobic GPC
228
Anaerobic GPR with molar tooth, affects brain/facw
Actinomyces
229
Two clostridium that have oval subterminal spores
botulinumdifficile
230
Clostridium doesn't have spores
perfringens
231
Clostridium with round terminal spore
ramosum
232
Anaerobic GPR antibiotic ass. diarrhea
C.difficile
233
Anaerobic GPR NF skin, acne
Propionibacterium
234
Anaerobic GPR NF mouth/female genital, lactic acid/pH
Lactobacillus
235
Anaerobic GNCNF upper, red flouresnce
Veillonella
236
Anaerobic GNR lung infection, tapered, fluoresence chartreuse/green yellow
Fusobacterium
237
Anaerobic GNR head,neck flurousence brick red
Prevotella
238
3 nonmotile
Shigella, KlebsiellaB.antracis (rest bacillus are motile)
239
Tuft of flagela at polar end
Lophitrichous
240
Uniform flagella over body
Petitrichous
241
Amphitrichous
Flagella at both poles
242
Have capsules: Most are respiratory CKSHN
Crypto neoKleb pneumoS.pneumoH.influN.memin
243
3 stains for metachromatic granules, best oneMAN
Methylene blue bestAlbertNeisser
244
Forms of sterilization
Autoclave 121, 15, 15Filtration Boiling Fractionalization Dry heatEthylene oxide gas
245
Gram stain ingredients
Crystal violetIodineEthyl alcohol Safranin
246
Ziel/kinyoun stain ingredients
Carbol fushin/phenolHCl/alcoholMethylene blue
247
Naso swabs used for these two
H.influN.mening
248
Urine cultureProcess time...Loop size, multiply by...Sig CFU...
1hr, 24hr fridge0.001ml, x1000>100,000 sig
249
Sputum stain, best sample req
Gram<10 epi, >25 PMN/lpf
250
ChromagarOxacillin, Cefoxitin used for
MRSA
251
Diff microccous from Strep
CatalaseMicrococcus cat pos
252
Staph epi from s.sapro
Novobiocin susceptible
253
Bacitracin susceptible PYR pos
GAS
254
Oxiase positive PVNM-CH
PseudomonasVibrio chNeisseriaM.cattCampyHeli,Haemo
255
Genital cultureTransport with these 2, AtCulture w/in, on
Transport modified Stuart's or Amies charcoal at RTCulture with in 12hrs on JEMBEC
256
Agar melts at, solidify at
Melts 100Solidify 45-50
257
Four ingredients in modified TMVCN, TL
VancomycinColistinNystatinTrimethoprim Lactate
258
Nitrocefin is a...Used to detect...Turns..
Chromogenic cephalosporinDetect cleavage of beta lactam ringRed
259
X factorV factor
X Hemin V NAD
260
Needs X,Vsatelitte phenomkidsmeningitis,epiglotitis
H.influe
261
Diff L.mono from GrpB strep
L.mono is motile and catalase pos
262
Two microbes that are CAMP and hippyrate pos
GrpBL.mono
263
Salicin used to diff these two.. which one pos
L.mono from Corynebacterium L.mono salicin pos
264
GPR partially acid fast, beaded, branching, filamentous. Urease positive
Nocardia
265
Curved Gram negative rod, gull winged MicroaerophillicNeeds increased Nitrogen42C
Campylobacter
266
Campy reqAtmosphere, temp
Microaerophillic 85 N10 Co25 O42C
267
Aspects of enteriobacteriace
GNROxidase negativeCatalase posNitrate to nitriteMotile except for kleb,shig,yer Ferments glucose, acid
268
Selective media for S&S with bromthymol blue and acid fuchsin as indicators
HE
269
GPR, spores, nonmotileString of pearls w/penicillin, Medusa
B.anthracis
270
Urease +PUNCH
ProteusUreaplasmaNocardiaCrytoneo,CorynebacHelipylori
271
Media where Mercury droplets can be seen in B.pertussis
Bordet gengou
272
E.Coli IMViC
++--
273
Kleb IMViC
--++
274
Most common cause of UTI females
Ecoli
275
Selects with Crystal violet, bile salts....diff with lactose
MAC
276
Selects with eosin Y, methylene blue...diff w/sucrose and lactose
EMB
277
Selects with bile salts, diff lactose/sucrose/salicin and has H2S indicator
HE
278
What are clear and colorless on HE? What is clear with black dot?
Shigella clearsalmonella clear w/black dot
279
What color are NF from the intestine on HE
orange/pink
280
What is the H2S indicator in He, SS, and XLD
Sodium thisulfate
281
selects with brilliant green, biles salts and diff with lactose and H2S
SS
282
Selects witih bile salts, diff with lactose/surcrose/xylose and H2S
XLD
283
Two enterobact that produce H2S
Salmonella and proteus
284
Enterobactero DNase pos and red
Serratia
285
Deaminase pos and Rapid urease posPPM
ProteusProvidenciaMoroganella
286
PPM are all pos/have these two enzymesProteusProvidenciaMoroganella
deaminase, urease
287
Typhoid fever caused by
Salmonella typhi
288
Positive nitrate test is...confirm with...
Clearzinc powder
289
TSI yellow butt
glucose
290
Yellow slant
lactose or sucrose
291
A/A which sugars
slant: lactose and/or sucrosebutt: glucose
292
K/A
butt: glucose only
293
K/K
none
294
black butt
H2S
295
Which test needs trytophan and is called Kovacs reagent
Indole
296
Citrate tests for microbes that
use citrate for their sole source of carbon
297
Kleb is citrate...
posIMViC --++
298
E.coli is citrate
negIMViC++--
299
Indicator that tests the ability of microbes to make glucose and drop the pH
methyl red
300
EMB selects and diff these group of microbes
enterobactericae
301
EMB has lactose to diff, what color is E.colicolor of NLF
green, LFclear
302
Test that tests microbes ability to make acetone from glucose
Voges Proskauer
303
Voges Proskauer2 pos: K/E2 Neg: E.cY
Pos: Kleb, EnterobacNeg: E.coli, Yer
304
In PPA, phenylalanine is deaminated to...three PPA pos...PPM
phenylpyruvid acidProteusProvidenciaMorganella
305
Enzyme that breaks down aminos to amines (alkaline) w/limited glucosefirst ph turns yellowwhen decarboxylized alkaline amine turns purple
Decarboxylase
306
Aminos used in decarboxylase test
LysineArginineOrthinine
307
Positive nitrate reduction test changes what color and inidicates...
positive changes red, indicates has only nitrate reductase
308
Initial no color in negative nitrate reduction test indicates
No color may mean microbe has both or neither enzymes nitrate/nitrite reductase
309
How to confirm no color reaction in nitrate reduction test....red..no color..
add powered zinc, will catalyze nitrate to nitritered...has neither enzymeclear...has both nitrite/nitrate
310
To ferment lactose need these two enzymes
permease: brings into cellB-galactosidase: breaks down lactose
311
What are microbes called that lack permease but have B-galactosidase
slow lactose fermenters
312
Which test detects B-galactosidaseWhat does pos look like
ONPGit resembles lactosepos=yellow
313
Sodium deoxycholate is used in
String test for vibrio choloera
314
Test/medium that uses low peptone to allow visible acid production by oxidizers
OFoxidition fermentation medium
315
With OF medium, what color is fermenters...where is the color for oxidizers...
acid, yellowoxidziers only on top due to needing oxygen
316
What is the indicator in OF media
bromthymol blue
317
Clostridium spores:oval/sub: bot,diffround: ramnone: perf
Clostridium spores:oval/sub: bot,diffround: ramnone: perf
318
Obligate intracellular parasite w/anthropod vectorGNCB that resemble bacteriaWeil-Felix (proteus ag)PCR lab titer
Rickettsia
319
Coxiella Burretii
obligate intracellular parasiteQ feveraerosol from birth of infectedbioterrism
320
Two genus that have intracellular morulae in cells and transmitted by ticks
Ehrlichia chaffeensis:mono/neuAnaplasma phagocytophilum:grans
321
Lyme disease, bulls eye rashIxodes damminiserological
Borelia burgedorferi
322
obligate intra parasiteSTD:parrots:PCR
Chlamydiatrachomatispsittaci
323
D test
double diffusionD appearance due to flattened zone of inhibition, resistant to clindamycin
324
Disk diffusion req
agar 4.0mmMH 150mmno more than 12 disksno CO2pH 7.2-7.4Ca/Mg
325
Ingredient in McFarland
Barium Chloride
