BACTE LEC BIOCHEMICAL TEST Flashcards
(150 cards)
1
Q
Tests designed to demonstrate the Physiological and chemical characteristics of microorganisms, by elimination to identify the microorganisms specifically
A
BIOCHEMICAL TESTS
2
Q
Demonstrates the enzyme system of a bacterial cell wall
A
BIOCHEMICAL TESTS
3
Q
Biochemical tests are based on the _____________ of an organism
A
PHENOTYPE
4
Q
Serological test that identifies strains of microorganisms that differ in their antigenic composition
A
Serotyping
5
Q
Test based on the characterization of microbial proteins
A
Matrix Assisted laser desorption/ ionization-time of flight mass spectrometry (MALDI-TOF MS)
6
Q
Used to enhance the recovery and identification of enteric bacteria
A
Molecular Diagnosis
7
Q
It involves the use of DNA probes and nucleic acids amplification
A
Molecular Diagnosis
8
Q
The fermentation of sugar is detected by acid production that yields a change of color in the culture medium
A
Carbohydrate utilization
9
Q
test used to determine whether Gram-positive rod utilizers glucose and/or lactose fermentatively or has no reaction
A
Carbohydrate Utilization Test
10
Q
facilitates the entry of lactose through the bacterial cell wall
A
B-galactosidase permease
11
Q
Hydrolyzes lactose in to glucose and galactose
A
B-galactosidase
12
Q
Anaerobic breakdown of carbohydrate resulting often in the production of large amounts of __________________
A
ORGANIC ACIDS
13
Q
Common carbohydrates used in culture media
A
GLUCOSE
LACTOSE
SUCROSE
14
Q
utilization of carbohydrate aerobically
A
OXIDATION
15
Q
utilization of carbohydrate anaerobically
A
FERMENTATION
16
Q
bacteria that produce a single acid
A
HOMOLACTIC ACID FERMENTERS
17
Q
bacteria that produce several different acids
A
MIXED ACID FERMENTERS
18
Q
What are the rapid lactose fermenters
A
(EKE)
E. coli
Klebsiella spp.
Enterobacter spp.
19
Q
What are the late lactose fermenters
A
(Cool Scientists Yearn for Helpful Studies & Solutions)
Citrobacter
Serratia
Yersinia enterocolitica
Hafnia alvei
Salmonella arizonae
Shigella sonnei
20
Q
pH indicators used for Carbohydrate Utilization
A
Bromcresol purple
Andrade’s fuchsin
Phenol red
21
Q
Medium used for the Carbohydrate utilization test
A
Liquid or semisolid meat extract broth to which an indicator and carbohydrates are added
22
Q
pH indicator for TSIA test
A
Phenol red
23
Q
H2S indicator for TSIA test
A
ferrous sulfate and sodium thiosulfate
24
Q
Inoculum technique for TSIA test
A
stab and Streak (butt-slant)
25
Ratio of CHO content for TSIA test
10:10:1
(1% lactose, 1% sucrose, 0.1% glucose)
26
TSIA reactions should not be read beyond ________________ of incubation
24hours
27
characteristic of uninoculated TSI agar
Red at pH 7.4
28
carbohydrates in the butt portion of the TSI agar
glucose
29
carbohydrates in the slant portion of the TSI agar
Sucrose and Lactose
30
Result: Alkaline slant/ Acid butt without black precipitate and gas
K/A
Neg H2S
Neg Gas
31
Result: Alkaline slant/ Alkaline butt
K/K
32
This reaction in TSIA test is typical among the non-fermentative organisms
K/K (no fermentation of sugar)
33
This reaction in TSIA test is when Sucrose and lactose are not fermented
K/A
34
Bacteria that will have the ff. result when tested in TSIA test:
K/A
Neg H2S
Neg Gas
SPSE
Shigella, Providencia, Serratia, Anaerogenic E. coli
35
Result: acid slant/ Acid butt with gas
A/A
Neg H2S
Pos Gas
36
This reaction in TSIA test is when all sugars are fermented
A/A
37
Bacteria that will have the ff. result when tested in TSIA test:
A/A
Neg H2S
Pos Gas
EKE
Escherichia, Klebsiella, Enterobacter
38
Result: Alkaline slant/ Acid butt with black precipitate and gas
K/A
Pos H2S
Pos Gas
39
Bacteria that will have the ff. result when tested in TSIA test:
K/A
Pos H2S
Pos Gas
SPCE
Salmonella, Proteus, Citrobacter, Edwardsiella
40
Result: acid slant/ Acid butt with gas. All sugars are fermented
A/A
Neg H2S
Pos Gas
41
Bacteria that will have the ff. result when tested in TSIA test:
A/A
Pos H2S
Pos Gas
Citrobacter freundii, Proteus, Salmonella
42
PAD test culture medium
Phenyl ethyl Alanine Agar
43
PAD test positive reaction
Green-colored complex on the slant
44
Quality control for TSIA A/A Gas (+)
E. coli
45
Quality control for TSIA K/A H2S (+)
Proteus mirabilis
46
Quality control for TSIA K/A Gas(+/-), H2S (+)
Salmonella serotype, tryphimurium
47
Quality control for TSIA K/A
Shigella flexneri
48
Quality control for TSIA
K/K
Pseudomonas aeroginosa
49
Quality control for TSIA
A/A H2S (-)
EKE
E. coli
Klebsiella spp.
Enterobacter
50
Quality control for TSIA
K/A H2S (+)
SPACED
Salmonella
Proteus
Arizonae
Citrobacter freundii
Edwardsiella tarda
51
Quality control for TSIA
K/A H2S (-)
YES SPMC
Yersinia pestis
E. coli
Serratia spp.
Shigella spp.
Providencia
Morganella
Citrobacter spp.
52
PAD test stands for
Phenylalanine Deaminase test
53
TSIA test stands for
Triple Sugar Iron Agar test
54
PAD test contents
0.2% phenylalanine
55
PAD test confirmatory reagent
10% ferric chloride
56
IMVIC test stands for
Indole
Methyl red
Voges Proskauer
Citrate
test
57
the lysis of the substrate tryptophan by the bacterial enzyme forms _____
indole
58
Reagent for the Indole portion in IMVIC test
PDAB (p-dimethylaminobenzaldehyde)
59
positive result for the Indole portion in IMVIC test
Red Coloration of the topmost layer
60
negative result for the Indole portion in IMVIC test
Yellow Coloration of the topmost layer
61
Two methods of Indole portion in IMVIC test
KOVACs Method
EHRLICHs Method
62
Medium: Tryptone broth or Trypticase broth
Positive Result: Development of a pink to deep red color
KOVACs Method
63
Medium: Tryptone broth or Trypticase broth with XYLENE
Positive Result: Brilliant red ring between the solvent and the medium
EHRLICHs Method
64
more sensitive method in Indole test
Ehrlich's Method
65
Indole test involving the ff:
Reagent: PDAC (p-Dimethylaminocinnamaldehyde)
Positive Reaction: Blue Color
SPOT INDOLE test
66
INDOLE (+) Microorganisms
May PEKPEC Po
Morganella
Proteus vulgaris
E. coli
Klebsiella oxytoca
Providencia
Edwardsiella tarda
Citrobacter koseri
Plesiomonas
67
test that determines the ability of the organism to produce and maintain stable acid end products (pyruvic acid) from glucose fermentation
METHYL RED and VOGES PROSKAUER test
68
Methyl Red test positive products and reaction
MIXED ACID FERMENTATION (Acidic Pathway)
Products: FLAS (Formic acid, Lactic Acid, Acetic Acid, Succinic Acid)
Reaction: Bright Red Color in addition of Methyl Red indicator
69
Vogues Proskauer test positive products and reaction
BUTYLENE GLYCOL PATHWAY (Neutral Pathway)
Products: Acetyl Methyl Carbinol (Acetoin)
Reaction: Red Color in addition of Barritt's A and B
70
MEDIUM for MRVP test
Clark and Lubs Medium/ MRVP broth/ Peptone glucose broth
71
INCUBATION for MRVP test
3-5 days @ 35 deg C
72
Quality Control for MRVP test: MR (+)/ VP (-)
E. Coli
73
Quality Control for MRVP test: MR (-)/ VP (+)
Enterobacter aerogenes
74
MRVP negative reaction
Yellow Color
75
VP positive microorganisms
C KEESH
Chronobacter sakazakii
Klebsiella
Ewingella
Enterobacter
Serratia
Hafnia
76
Test that determines the ability of an organism to utilize sodium citrate as its only carbon source and inorganic ammonium salts as its only nitro source
SIMMONS CITRATE test
77
Medium used for Simmons Citrate Test
Simmons Citrate
78
pH indicator used for Simmons Citrate Test
Bromothymol Blue
79
Negative reaction for Simmons Citrate Test
Green color (Acidic 6.9)
80
Positive reaction for Simmons Citrate Test
Blue Color (Alkaline 7.6)
81
Positive control microorganism used for Simmons Citrate Test
Enterobacter aerogenes
82
Negative control microorganism used for Simmons Citrate Test
Escherichia coli
83
CITRATE test involving the ff:
pH indicator: Phenol Red
Negative Reaction: Yellow
Positive Reaction: Pink (Alkaline pH)
CHRISTENSEN CITRATE MEDIUM
84
Citrate (+) microorganisms
Pero Sa CHEEKS
Providencia
Serratia
Citrobacter
Hafnia
Ewingella
Enterobacter
Klebsiella
Salmonella enteritidis
85
test used to determine whether an enteric organism is motile; must have a flagella
MOTILITY test
86
medium used for Motility Test
SIM (Sulfur Indole Motility) Medium
Semisolid motility medium
87
Reagent to aid in visualization in Motility test
1% triphenyletrazolium chloride
88
Positive motility reaction for Motility test
movement away from the stab line creating a hazy appearance
89
Positive Control microorganism for Motility Testing
Escherichia coli
90
Negative Control microorganism for Motility Testing
Staphylococcus aureus
91
Non motile group under 37 deg C
SKY
Shigella
Klebsiella
Yersinia enterocolitica
92
test where the principle is on the degradation of amino acids and their specific end products
DECARBOXYLASE TEST
93
End product of lysine after decarboxylation
Cadaverine + CO2
94
End product of Ornithine after decarboxylation
Putrescine
95
test that measures the enzymatic ability of an organism to decarboxylate (hydrolyze/ remove) an amino acid to form amine (putrescine or cadaverine) in an anerobic environment
DECARBOXYLASE TEST
96
Decarboxylase test base medium
Glucose and amino acids
1% Lysine
1% Ornithine
1% Arginine
97
Reagent for Decarboxylase test to promote anaeobiosis
4mm mineral oil
98
pH Indicator for Decarboxylase test
Bromcresol purple and cresol red
99
negative reaction for Decarboxylase test
Yellow Color
100
positive reaction for Decarboxylase test
Purple color
101
Positive control microorganism in Decarboxylase test for:
Arginine
Lysine
Ornithine
Arginine: Pseudomonas aeruginosa
Lysine: Klebsiella pneumoniae
Ornithine: Enterobacter aerogenes
102
Negative control microorganism in Decarboxylase test for:
Arginine
Lysine
Ornithine
Arginine: E. coli
Lysine: Citrobacter freundii
Ornithine: Proteus vulgaris
103
glucose fermenter control microorganism in Decarboxylase test
Klebsiella pneumoniae
Enterobacter aerogenes
104
LIA test stands for
Lysine Iron Agar test
105
used to determine whether a Gram-negative rod decarboxylates or deaminates lysine and produce H2S gas
Lysine Iron Agar Test
106
Medium used for LIA test
Lysine Iron Agar (Slant and butt)
107
Inoculation technique used for LIA test
Stab and Streak
108
Major component for LIA test
Lysine, Peptones, and 0.1% glucose
109
H2S indicator used for LIA test
Ferric Ammonium citrate and Sodium thiosulfate
110
pH indicator used for LIA test
Bromocresol purple
111
Positive reaction for slant in LIA test
Red/ Burgundy Color
112
Positive reaction for butt in LIA test
Purple Color (No fermentation of glucose)
113
The test being conducted in the slant of the LIA test
Lysine Deamination
114
The test being conducted in the butt of the LIA test
Lysine Decarboxylation
115
LIA test microorganism for ff results:
K/K
Lysine deamination (-)
Lysine decarboxylation (+)
Salmonella
116
LIA test microorganism for ff results:
K/A
Lysine deamination (-)
Lysine decarboxylation (-)
Shigella and Citrobacter
117
LIA test microorganism for ff results:
R/A
Lysine deamination (+)
Lysine decarboxylation (-)
Proteus, Providencia, Morganella
118
ONPG test stands for
Ortho-Nitrophenyl-B-D-Galactopyranoside-Test
119
Test that determines the presence of late or slow lactose fermenting strains
ONPG test
120
Test that differentiates organisms which can produce B-galactosidase
ONPG test
121
Positive reaction for ONPG test
Yellow Color
122
Positive control microorganism for ONPG test
Shigella sonnei
123
negative control microorganism for ONPG test
Salmonella serotype, Tryphimurium
124
Test to determine the ability of an organism to reduce nitrate to nitrite
Nitrate reduction test
125
Medium used for Nitrate Reduction test
Peptone broth with KNO3
126
Reagents used for Nitrate Reduction test
Sulfanilic Acid and A-naphthylamine
127
reagent used to confirm negative nitrite result in Nitrate Reduction test
Zinc Dust
128
Positive reaction for Nitrate Reduction test
Red Color
129
Negative reaction for Nitrate Reduction test
Colorless
130
Positive control microorganism for Nitrate Reduction test
Proteus mirabilis
Kingella denitrificans
131
Negative control microorganism for Nitrate Reduction test
Acinetobacter baumanii
Neisseria gonorrheae
132
test that detects the ability of an organism to secrete urease which hydrolyzes urea
Urease Test
133
Positive Result for Urease Test
Pink Color
134
Rapid urease producers (within 1-2 hours)
TP PUNCH
Proteus
Providencia
Morganella
Ureaplasma
Nocardia
Helicobacter pylori
T. mentagrophtes
C. neoformaris
135
Slow urease producers (within 4 hours)
CKEYS
Citrobacter
Klebsiella
Enterobacter
Yersinia
Serattia
136
test that determines the production gelatinase (protease) which hydrolyzes and liquifies the gelatin the medium
Gelatin Liquefaction test
137
Medium used for Gelatin Liquefaction test
Nutrient gelatin deep tubes that contain 12% gelatin
138
Incubation for Gelatin Liquefaction test
48 hours incubation @ 37 deg C, 30 mins refrigeration
139
Positive reaction for Gelatin Liquefaction test
Liquefied gel
140
Negative reaction for Gelatin Liquefaction test
Solid gel
141
Positive control microoranisms for Gelatin Liquefaction test
Serratia and Proteus
142
employs strips of clear blue plastic covered with a gray-green coating of gelatin
Gelatin Strip Method
143
Positive reaction for Gelatin Strip Method
gelatin coating is slowly hydrolyzed and the blue plastic strip becomes visible
144
Negative Gelatin Strip Method
no blue plastic visible on the gelatin strip
145
test that identifies the ability of the organism to utilize sodium malonate as its sole source of carbon
Malonate test
146
Medium used for Malonate test
Malonate Broth
147
pH indicator used for Malonate test
Bromothymol blue
148
Positive reaction for Malonate test
Blue color @ 7.6 pH
149
Negative reaction for Gelatin Strip Method
Green color
150
Positive control microorganisms for malonate test
Enterobacter and Salmonella
