Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

2A MIDTERMS > BACTE NFO 2 > Flashcards

BACTE NFO 2 Flashcards

(54 cards)

Start Studying
1
Q

NONFERMENTING
GRAM NEGATIVE BACILLI usually presents oxidase ____ reaction

A

Positive

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

NONFERMENTING
GRAM NEGATIVE BACILLI presents resistance to the ff. antimicrobials

A
  • aminoglycosides
  • third-gen cephalosporin
  • penicillin
  • fluoroquinolones
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

How to classify Non Fermenters based on Biochem reaction

A

★ Growth on MAC
★ Oxidase reaction
★ Glucose OF Test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

★ Obligate aerobe, motile, and rod shape
★ Grows at 37 – 42 oC, grows well at 35
oC
★ Colonies have a grape-like or corn-taco or fruity
odor

A

PSEUDOMONAS AERUGINOSA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Colonies of PSEUDOMONAS AERUGINOSA have a grape-like or corn-taco or fruity
odor due to _______________

A

2- aminoacetophenone

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

DISEASE ASSOCIATED with P. aeruginosa

A

★ Ventilator-associated pneumonia
★ Jacuzzi or hot tub syndrome
(necrotizing rash)
★ Otitis media (swimmers or divers)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

P. aeruginosa Pigments

A

○ Pyorubin (red)
○ Pyocyanin (blue)
○ Pyoverdine (green)
○ Pyomelanin (black or brown)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Bacteria that presents a flat, spreading, mucoid colonies serrated
edges, metallic sheen in BAP

A

P. aeruginosa

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

P. aeruginosa reaction in MAC

A

NLF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Color of P. aeruginosa in Nutrient Agar

A

Green

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

P. aeruginosa biochem test

A

➔ Oxidase (+)
➔ Pyocyanin (+)
➔ Fluorescein (+)
➔ Motility (+)
➔ TSI: K/K
➔ Gelatin Hydrolysis (+)
➔ Nitrate Reduction (+)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Cetrimide Agar stimulators

A

magnesium chloride and potassium
sulphate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Cetrimide Agar positive reaction

A

yellow green to blue colonies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Cetrimide agar Inhibitor

A

cetyltrimethylammonium bromide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Associated with pneumonia in patients with
Cystic fibrosis or Chronic granulomatous
disease

A

BURKHOLDERIA CEPACIA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Identify Bacteria based on the ff:

➔ Oxidase (+)
➔ Pyocyanin (-)
➔ Fluorescein (-)
➔ Motility (+)
➔ Gelatin Hydrolysis (V)
➔ Nitrate Reduction (V)
➔ Fermented Sugars
> Glucose (+)
> Lactose (+/V)
> Mannitol (V)
> Xylose (+/V)

A

BURKHOLDERIA CEPACIA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

BURKHOLDERIA CEPACIA odor character

A

Dirt Like Odor

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Bacteria that presents a smooth, slightly raised, yellow to brown,
sometimes beta hemolysis strains in BAP

A

BURKHOLDERIA CEPACIA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

BURKHOLDERIA CEPACIA in MAC

A

NLF (Older colonies sometimes cause false positive)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

SELECTIVE MEDIA for BURKHOLDERIA CEPACIA

A
  • Burkholderia Cepacia Selective Agar (BCSA)
  • Pseudomonas Cepacia (PC) Agar
  • OF BASE, POLYMYXIN B,
    BACITRACIN AND LACTOSE (OFBL)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

is an agar that has improved the recovery and isolation of B.
cepacia when compared to other selective
mediums

A

OF BASE, POLYMYXIN B, BACITRACIN AND LACTOSE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

OFBL positive reaction

Study These Flashcards
A

yellow colonies with yellow halos

23
Q

causative agent of Glanders Disease (Farcy Disease)

Study These Flashcards
A

BURKHOLDERIA MALLEI

24
Q

Identify Bacteria based on the ff:

➔ Oxidase (+)
➔ Pyocyanin (-)
➔ Fluorescein (-)
➔ Motility (-)
➔ Gelatin Hydrolysis (-)
➔ Nitrate Reduction (+)
➔ Fermented Sugars
> Glucose (+)
> Lactose (V)
> Mannitol (-)
> Xylose (V)

Study These Flashcards
A

BURKHOLDERIA MALLEI

25
Agent of Vietnamese Time Bomb or Melioidosis (an endemic Glanders-like disease)
BURKHOLDERIA PSEUDOMALLEI
26
BURKHOLDERIA PSEUDOMALLEI other name
whitmore bacillus
27
Identify Bacteria based on the ff: ➔ Oxidase (+) ➔ Pyocyanin (-) ➔ Fluorescein (-) ➔ Motility (+) ➔ Gelatin Hydrolysis (V) ➔ Nitrate Reduction (+) ➔ Fermented Sugars > Glucose (+) > Lactose (+) > Mannitol (V) > Xylose (+)
BURKHOLDERIA PSEUDOMALLEI
28
Bacteria with cream to yellow-orange, smooth and mucoid for young colonies (24 – 48 hours) and dry to wrinkled ( > 3 days); nonhemolytic; putrid odor in BAP
BURKHOLDERIA PSEUDOMALLEI
29
BURKHOLDERIA PSEUDOMALLEI in MAC reation
NLF but appears pink (oxidize lactose)
30
BURKHOLDERIA PSEUDOMALLEI selective media
Ashdown Medium
31
BURKHOLDERIA PSEUDOMALLEI preferred specimen
throat, rectal, sputum
32
Ashdown Medium inhibitor
crystal violet and gentamicin
33
Ashdown Medium indicator
neutral red
34
Ashdown Medium positive reaction
pinkish-purple, flat and dry colonies
35
Causes severe lung infections, bacteremia in cystic fibrosis patients
Burkholderia cepacia complex
36
Causes melioidosis, skin abcess
Burkholderia pseudomallei
37
Causes localized suppurative or pulmonary infections
Burkholderia mallei
38
Associated with cystic fibrosis patients, opportunistic infection
Burkholderia gladioli
39
Causes recurrent meningitis and cystic fibrosis patients
Ralstonia spp.
40
Causes bacteremia, peritonitis, infection in cystic fibrosis patients
Cupriavidus spp.
41
★ Most common Acinetobacter species seen in clinical specimen
A. baumannii (glucose oxidizing)
42
A. baumanni is _______________ resembles lactose fermenting organism
SACCHAROLYTIC
43
ASACCHAROLYTIC ACINETOBACTER spp.
A. iwoffii (glucose non-oxidizing)
44
Most hemolytic strain of Acinetobacter
A. haemolyticus
45
Was known as “MIMA”, because it was said to mimic the appearance of neisseria in the gram stain in female genital tract specimens
A. baumannii
46
Acinetobacter in MAC
bluish or peach to pink
47
translucent appearance large, gummy, translucent, gray-to-white, convex, entire colonies
Acinetobacter
48
A. baumannii in EMB
cornflower blue color colony
49
★ Third most common non-fermentative, gram-negative bacillus ★ Found in water, sewage and plant materials ★ Oxidase negative, catalase negative
STENOTROPHOMANS MALTOPHILIA
50
STENOTROPHOMANS MALTOPHILIA drug of Choice
SXT
51
Bacteria with lavender green to light purple pigment in BAP
STENOTROPHOMANS MALTOPHILIA
52
Identify Bacteria based on the ff: ➔ Oxidase (-) ➔ Motility (+) ➔ Nitrate Reduction (V) ➔ Fermented Sugars > Glucose (+) > Maltose (+)
STENOTROPHOMANS MALTOPHILIA
53
Identify Bacteria based on the ff: ➔ Oxidase (-) ➔ Motility (-) ➔ Nitrate Reduction (-) ➔ Fermented Sugars > Glucose (+) > Maltose (-)
Saccharolytic A. baumannii
54
Identify Bacteria based on the ff: ➔ Oxidase (-) ➔ Motility (-) ➔ Nitrate Reduction (-) ➔ Fermented Sugars > Glucose (+) > Maltose (V)
Asaccharolytic A. iwoffii

2A MIDTERMS (20 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions