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2A MIDTERMS > BACTE LEC STREP 2 > Flashcards

BACTE LEC STREP 2 Flashcards

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1
Q

Positive for Bacitracin Test

A

S. pyogenes
M. luteus

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2
Q

Negative for Bacitracin Test

A

S. agalactiae
S. aureus
S. viridans streptococci

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3
Q

S. pyogenes Specimens of Choice

A

Throat Swab/ Pharyngeal and Tonsillar swabs

Tissue Biopsy

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4
Q

used to differentiate group B strep, from other streptococcal species

A

CAMP test

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5
Q

CAMP test principle states that Organisms that produce a diffusible extracellular hemolytic protein (CAMP Factor) that acts synergistically with the beta-lysin of Staphylococcus aureus to cause ______________________ of red blood cells

A

enhanced lysis

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6
Q

Bacteria that will yield positive to CAMP test

A

S. agalactiae or Group B Strep
L. monocytogenes

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7
Q

Positive result for CAMP test

A

enhanced hemolysis indicated by arrow head shaped zone of B-hemolysis

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8
Q

Bacteria that is negative for CAMP test

A

S. pyogenes

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9
Q

Test for detecting the enzyme hippuricase (aka Hippurate hydrolase)

A

Hippurate Hydrolysis

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10
Q

What is the positive result for Hippurate Hydrolysis Test

A

Formation of deep purple end product

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11
Q

Bacteria that is positive for Hippurate Hydrolysis Test

A

S. agalactiae

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12
Q

Bacteria that is Negative for Hippurate Hydrolysis Test

A

S. pyogenes

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13
Q

Limitations of Hippurate Hydrolysis Test

A

false positive result may occur when incubation with ninhydrin is > 30mins

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14
Q

Bacteria that is a part of the normal microbiota of the female genital tract and lower gastrointestinal tract (occasional colonizer of upper RT)

A

S. agalactiae (G-B)

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15
Q

Colonies characteristic in BAP of S. agalactiae

A

Grayish/ white mucoid colonies surrounded by a small/ narrow zone of b-hemolysis

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16
Q

important virulence factor that prevents phagocytosis but is ineffective after opsonization

A

CAPSULE

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17
Q

is the most significant component of the capsule and a critical virulence determinant

A

SIALIC ACID

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18
Q

S. agalactiae is the common cause of _______________

A

neonatal meningitis

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19
Q

most important determining factor in early-onset infection of neonatal meningitidis

A

Presence of GBS in the vagina of the mother

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20
Q

All pregnant women should be screened for GBS at ______________ gestation

A

35-37 weeks gestation

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21
Q

detection of GBS in pregnant women is accomplished by collecting __________________ with swabs between 35-37 weeks of gestation

A

vaginal and rectal material

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22
Q

Broth used for the growth of S. agalactiae

A

Todd-Hewitt Broth
Strep B Carrot Broth

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23
Q

Broth used for the growth of S. agalactiae that produce an orange or red pigment after 6 hours

A

Strep B Carrot Broth

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24
Q

test for the presumptive identification of enterococci and organisms in the Streptococcus bovis group

A

Bile Esculin Test

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25
used for differentiation of enterococci and Group D streptococci form non- group D viridans streptococci
Bile Esculin Test
26
Bile esculin test is a ____________________________ agar used to isolate and identify _____________________ and the ______________
selective differential group D streptococci enterococci
27
in Bile Esculin test the selective ingredient that inhibits the growth of most gram positive organisms is ________________, while ____________ is the differential component
Oxgall (bile salt) Esculin
28
Positive result for the Bile Esculin Test
Growth and blackening of the agar slant
29
bacteria positive in Bile Esculin Test
E. faecalis
30
bacteria negative in Bile Esculin Test
E. coli S. pyogenes
31
Streptococci that is a part of normal microbiota of the skin, nasopharynx, GI tract and genital tract
Group C and G Streptococci
32
causes infections that resemble S. pyogenes
Group C and G Streptococci
33
used to determine the ability of an organism to grow in high concentrations of salt, also used to differentiate enterococci from nonenenterococci
Salt (6.5%) Tolerance Test
34
Positive result for Salt Tolerance Test
Visible turbidity in the broth, with out without a color change from purple to yellow
35
bacteria positive in Salt Tolerance Test
E. faecalis
36
bacteria negative in Salt Tolerance Test
Streptococcus gallolyticus
37
To avoid false negative result in Salt Tolerance Test, the broth should be ___________ before interpretation. On the other hand, inoculating the broth too heavily may give a _______________________ result
Gently mixed, false positive
38
bacteria that are natural inhabitants of the intestinal tract, which sometimes exhibit "pseudocatalase" reaction or weak bubbling in the catalase due to peroxidase
Enterococcus
39
enterococcus has acquired resistances to _______________ and __________________
Tetracyclines and vancomycin
40
enterococcus has intrinsic resistance to ___________, including the ______________ and __________________
Beta-lactam antibiotics penicillin cephalosporins
41
used to determine the effect of optochin on an organism,
optochin susceptibility test
42
is an antibiotic that interferes with the ATPase and production of adenosine triphosphate ion microorganisms
Optochin
43
Optochin is an antibiotic that interferes _____________ but alpha-streptococci are resistant
pneumococci
44
Positive result for Optochin susceptibility test
Zone of inhibition at least 14mm in diameter with 6mm disk
45
Equivocal result for Optochin susceptibility test
Any zone of inhibition less than 14mm is questionable for pneumococci
46
Positive result for Optochin Susceptibility test
S. pneumoniae
47
Negative result for Optochin Susceptibility test
S. pyogenes
48
test that differentiates S. pneumoniae from alpha hemolytic streptococci. It also correlates with optochin susceptibility
Bile solubility test
49
Positive result for Bile Solubility test
Colony disintegrates; an imprint of the lysed colony may remain in the zone
50
Bacteria that is positive for S. pneumonia
Streptococcus pneumonia
51
Negative that is positive for S. pneumonia
E. faecalis
52
A biochemical reaction in which a capsular antibodies bind to the capsule of a bacterium
Neufeld Quelling Test
53
Member of the much more virulent streptococcus group _____________
Streptococcus pneumoniae
54
Gram stain reaction and morphology of S. pneumoniae
Gram positive with lancet or bullet shaped
55
S. pneumoniae can be isolated using ________ medium and requires ______________
Sheep Blood Agar, 5-10% CO2
56
Colony morphology of S. pneumoniae
Small round, glistening, dome-shaped, colonies that are transparent, with an entire edge
57
hemolysis on S. pneumoniae may be attributed to ______________
pneumolysin
58
Colonies of S. pneumoniae exhibit a ________________ when incubated aerobically for 24 hours
wide zone of alpha-hemolysis
59
major virulence factor of S. pneumoniae
Polysaccharide capsule
60
enzyme of S. pneumoniae that degrades surface structures of host mucus membranes
Neuraminidase
61
enzymes of S. pneumoniae that facilitate bacterial colonization on mucosal surfaces by eliminating immunoglobulins such as IgA, IgG, and IgM
Ig proteases
62
an oxygen sensitive toxin that is cytolytic for cells
Pneumolysin O
63
facilitates the release of pneumolysin O and other toxic proteins or inflammatory substances from cells
Autolysin
64
virulence factor of S. pneumoniae that reacts with some components of the immune response resulting in the activation of some non specific host immune response
C- Substance
65
S. pneumoniae is the number cause of ________________________________, especially in the elderly persons and in patients with underlying diseases
Bacterial Pneumonia
66
bacteria that is a constituent of the normal microbiota of the upper RT, female genital tract and the Gi tract
Viridians Streptococci
67
The term viridans means _________ referring to the _______________________
Green Alpha-hemolysis
68
Viridans Streptococci produces a characteristic odor of ________________
Butterscotch, Sweet odor of honeysuckle especially in CHOC Agar
69
bacteria in the mitis group
S. mitis, S. pneumonia, S. sanguis, S. oralis
70
bacteria in the Mutans group
S. mutans, sobrinus
71
V. streptococci is the most common cause of ___________________, a condition associated with a transient bacteremia
subacute bacterial endocarditis
72
most common isolates/ group for isolating associated bacterial endocarditis in native valves
S. mitis group
73
a bacteria that when viewed has the highest correlation with gastrointestinal carcinoma
S. gallolyticus subsp. gallolyticus

2A MIDTERMS (20 decks)

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