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2A MIDTERMS > BACTE LEC HACEK > Flashcards

BACTE LEC HACEK Flashcards

(73 cards)

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1
Q

The term Haemophilus is greek word which means ________________

A

Blood Loving

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2
Q

Haemophilus requires preformed factors in the blood which are:

A

X factor
V factor

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3
Q

Preformed factor in the blood that is the only readily available

A

X factor/ Hemin

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4
Q

Preformed factor in the blood that is not readily available and requires exogenous sources

A

V factor/ NADP

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5
Q

In terms of oxygen requirement Haemophilus are __________________________

A

facultative anaerobes, but prefers to be capnophilic

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6
Q

_____________ agar is not suitable for Haemophilus

A

Sheep Blood Agar

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7
Q

_____________ agar can be used for Haemophilus, but is not well maintained in the laboratory

A

Horse or Rabbit Blood Agar

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8
Q

preferred isolation medium of Haemophilus

A

Chocolate Agar

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9
Q

Chocolate agar release ________ and inactivates ___________

A

Hematin (X factor), NADase

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10
Q

Synthetic CA is can be used and contains:

A

NAD, Iron, Vit B12, Thiamine, magnesium, glucose, cysteine, and glutamine

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11
Q

released in the degradation of the product of hemoglobin

A

X factor

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12
Q

X factor is heat ______

A

stable

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13
Q

Also known as NAD or COENZYME 1

A

V Factor

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14
Q

V factor is produced by:

A
  • Some bacteria (S. aureus)
  • Yeast
  • Potato & yeast extract
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15
Q

V factor is heat ______

A

labile

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16
Q

Test based on the ability of the organism to convert the substrate Delta- aminolevulenic acid (ALA) into porphyrins or porphobilinogen

A

Porphyrin (D-ALA) Test

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17
Q

Porphyrin (D-ALA) Test medium used

A

Porphyrin Broth Medium

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18
Q

Porphyrin (D-ALA) Test Substrate

A

Delta- aminolevulenic acid (ALA)

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19
Q

Porphyrin (D-ALA) Test reagent

A

PDAB

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20
Q

Porphyrin (D-ALA) Test result for porphobilinogen positive

A

RED COLOR after PDAB

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21
Q

Porphyrin (D-ALA) Test result for porphyrin positive

A

REDDISH ORANGE at 360nm UV

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22
Q

Test used for the differentiation of Haemophilus spp.

A

X and V factor Test

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23
Q

X and V factor Test medium used

A

Mueller Hinton Agar/ Brain Heart Infusion Agar

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24
Q

X and V factor Test reagent

A

X and V factor impregnated in disks

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25
X and V factor Test result for organisms that need X factor to Grow
Growth in X and XV factor disks
26
X and V factor Test result for organisms that need V factor to Grow
Growth in V and XV factor disks
27
X and V factor Test result for organisms that need X & V factor to Grow
Growth in XV factor disks only
28
H. influenzae Biochem test results
XV (Growth) ALA test (-) Haemolysis (-)
29
H. aegypticus Biochem test results
XV (Growth) ALA test (-) Haemolysis (-)
30
H. parainfluenzae Biochem test results
V (Growth) ALA test (+) Haemolysis (-)
31
H. haemolyticus Biochem test results
XV (Growth) ALA test (-) Haemolysis(+)
32
H. parahaemolyticus Biochem test results
V (Growth) ALA test (+) Haemolysis (+)
33
H. influenzae is also known as the ___________
Pfeiffers Bacillus
34
H. influenzae virulence factors
- CAPSULE - IgA PROTEASE - FIMBRIAE - LPS and Other Membrane Proteins - BETA- LACTAMASE Production
35
The main cause of meningitis in children <5 years old
H. influenzae
36
Main cause of Pink Eye Conjunctivitis
Haemophilus aegypticus
37
Haemophilus aegypticus is also known as _______________
Koch's Week's Bacillus
38
Main cause of STI- Soft chancre
Haemophilus ducreyi
39
has an appearance of schools of fish/ raid road tracks on gram staining
Haemophilus ducreyi
40
Odor of H. influenzae
Mousy or bleach-like odor
41
Haemophilus spp. that is a true pathogen
Haemophilus ducreyi
42
characterized as small, pleomorphic, non-motile, Gram negative Coccobacilli, that are facultative anaerobes, which require X and V factor to grow
Haemophilus spp.
43
Haemophilus spp. that is normally a part of the normal microbiota of the upper respiratory tract
H. influenzae, and others such as H. parainfluenzae, H. parahaemolyticus
44
Haemophilus spp. that is not a part of the normal human microbiota, only found in humans during infection
Haemophilus ducreyi
45
Two patterns of H. influenzae disease
Invasive Disease Localized Infection
46
this is usually caused by encapsulated strains of Haemophilus spp. causing septicemia, meningitis, arthritis, and pneumonia
Invasive Disease
47
this is usually caused by Non typable Haemophilus influenzae, conjunctivitis, sinusitis, otitis media
Localized infection
48
Heamophilus serotype that is likely to cause invasive disease
Serotype B
49
Haemophilus ducreyi incubation time
4-14 days
50
Haemophilus ducreyi most common sites
penis or labia
51
Haemophilus found in the oral cavity, and is the rare cause of ENDOCARDITIS
H. parainfluenzae
52
H. parainfluenzae primary site of infection
Mitral Valve
53
Selective agent for H. aegypticus to be added in CHOC
1% IsoVitale
54
Preferred growth media of H. ducreyi
Nairobi Biplate
55
H. aegypticus must be incubated/ isolated for _____ days
7 Days
56
H. ducreyi must be incubated/ isolated for _____ days
4 Days
57
HACEK stands for
Haemophilus Aggregatibacter Cardiobacterium Eikenella Kingella
58
Most prevalent HACEK species causing endocarditis
Aggregatibacter aphrophilus
59
causative agent for periodontitis
Aggregatibacter actinomycetemcomitans
60
Aggregatibacter that appears raised, convex, granular, yellowish
Aggregatibacter aphrophilus
61
Aggregatibacter that appears to have star shaped colonies
Aggregatibacter actinomycetemcomitans
62
HACEK that infects the aortic valve
Cardiobacterium hominis
63
HACEK spp. that may pit the agar, and presents a rosette formation in microscopy
Cardiobacterium hominis
64
Least common HACEK, causes mixed infection from bites and clenched fist wound
Eikenella corrodens
65
Eikenella corrodens is also known as the _____________
Corroding Bacilli
66
HACEK isolated form degenerative joints and bone infection in children <3 years old
Kingella kingae
67
HACEK that presents a sharp odor of bleach
Eikenella corrodens
68
HACEK that has the tendency to resist decolourization
Kingella kingae
69
HACEK Bacteria that is: Cat (-) Oxidase (V) Glucose (+) Maltose (+) Sucrose (+) Lactose (+)
A. aphrophilus
70
HACEK Bacteria that is: Cat (+) Oxidase (V) Glucose (+) Maltose (+) Sucrose (-) Lactose (-)
A. actinomycetemcomitans
71
HACEK Bacteria that is: Cat (-) Oxidase (+) Glucose (+) Maltose (+) Sucrose (+) Lactose (-)
Cardiobacterium hominis
72
HACEK Bacteria that is: Cat (-) Oxidase (+) Glucose (-) Maltose (-) Sucrose (-) Lactose (-)
Eikenella corrodens
73
HACEK Bacteria that is: Cat (-) Oxidase (+) Glucose (+) Maltose (+) Sucrose (-) Lactose (-)
Kingella kingae

2A MIDTERMS (20 decks)

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