Campylobacter & Helicobacter Flashcards
(14 cards)
1
Q
Campylobacter Species. & reservoirs
A
- C. Jejuni (poultry)
- C. Coli (pigs)
- C. Fetus (cattle/sheep)
2
Q
Campylobacter Info
A
- Cause zoonotic infections
- Infection through contaminated food
- C. Jejuni most common
3
Q
Campylobacter Morphology
A
- Gram Negative
- Curved rods (spiral)
- Microaerophilic
- Prefers 42˚C adapted to bird body temp
4
Q
Campylobacter Cultivation
A
Special medium called Skirow’s Medium
5
Q
Campylobacter Virulence Factors
A
Toxins damaging GI endothelial cell of blood vessels
6
Q
Campylobacter Diseases
A
- Gastroenteritis
- Bloody Diarrhea
- Reactive Arthritis
- Guillan-Barré syndrome: Demyelination, ascending paralysis
- Sepsis or bacteremia (invasive)
7
Q
Campylobacter Treatment
A
- Rehydration
- Macrolides if severe
8
Q
Campylobacter Microbiological Diagnosis
A
- Stool sample cultivation
- Microscopy: Thin, S-shaped bacteria
9
Q
Helicobacter Groups
A
- Gastric Helicobacter (pylori)
- Enterohepatic Helicobacter (cinaedi & finnelliae)
10
Q
H. Pylori Morphology
A
- Gram Negative
- Curved Rods (helical)
- Microaerophilic
- Flagella (motile)
- Fastidious to culture
- Silver stain used
- Urease +, Catalase +, Oxidase +
- Can ferment a.a
11
Q
H. Pylori Virulence
A
- Endotoxin (LPS)
- Adhesins
- Urease Enzyme (alkaline, NH3)
- Polar Flagella
- Cytotoxins (VacA, CagA)
12
Q
H. Pylori Diseases
A
- Acute/Chronic Gastritis
- Gastric & Duodenal Ulcer
- Increased Cancer risk (Gastric Adenocarcinoma, MALT Lymphoma)
- Only bacteria known to be oncogenic
13
Q
H. Pylori Treatment
A
Combined therapy for 2 weeks
- PPI
- Amoxicillin
- Clarithromycin
14
Q
H. Pylori Microbiological Diagnosis
A
- Urea Breath Test (radiolabeled Urea)
- Stool antigen test
- Gastric Biopsy + Silver impreg.
- Biopsy Fluorescent in Situ Hybridization (FISH)
