Listera, Erysipelothrix, Lactobacillus, Bifidobacterium, Pre/Pro-biotics Flashcards
(28 cards)
1
Q
Listeria Monocytogenes General info
A
- Zoonotic
- Milk / Dairy, deli cuts, ready-made salads, frozen vegetables
- Common in Pregnant, Neonates, & Elderly
2
Q
Listeria Morphology
A
- Gram Positive
- Rods
- Non-spore forming
- Flagella, Motile (rolling)
- Facultative Anaerobe
- Grows even at 4˚C
3
Q
Listeria Culturing
A
- Simple culture media
- B-Hemolysis
- Facultative I.C
- Can grow at low temps
4
Q
Listeria Non-Toxic Virulence factors
A
- Adhesins: Glycoprotein R, Internalin A/B (epith, M-cells peyers)
- ActA Protein: Actin polymerization for movement
- Flagella
- PrfA (pos reg factor)
5
Q
Listeria Toxic Virulence factors
A
- Listeriolysin O (pores)
- PLC
- Stress response genes to survive digestive enzymes
6
Q
Listeria Diseases
A
- Gastroenteritis in Healthy
- Pregnant Chorioamnionitis, Abortion, Sepsis
- Neonatal Meningitis, Granulomatosis Infantiseptica
7
Q
Listeria Treatment
A
- Listeria naturally resistant to Cephalosproins
- Ampicillin + Gentamycin (not in kids)
- Trimethoprim-Sulfamethoxazole (bactericidal)
- No vaccine
8
Q
Listeria Microbiological Diagnosis
A
- Culture specimen
- CAMP positive
- B-Hemolysis
9
Q
Erysipelothrix Rhusiopathaie General Info
A
- In wild & domestic animals (swine, turkey, fish)
- Zoonotic infection
10
Q
Erysipelothrix Morphology
A
- Gram Positive
- Rods
- Non-spore forming
- Encapsulated
- Non-motile
11
Q
Erysipelothrix Cultivation
A
- Slow growth in Microaerophilic condition
- a-Hemolysis on blood agar
12
Q
Erysipelothrix Virulence Factors
A
- Neuraminidase: colonization & penetration
- Capsule (antiphyagocytic)
13
Q
Erysipelothrix Diseases
A
- Erysipelas in Swine
- Erysipeloid in Humans (no pus)
14
Q
Erysipelothrix Treatment
A
Penicillin
(self-limiting disease)
- Vaccine for animals
15
Q
Lactobacillus Genus Info
A
- Used in Probiotics
- Over 200 Species
- L. Acidophilus, Fermentum, Casei, Delbrueckii
16
Q
Lactobacillus General Info
A
- Normal microbiota of Vagina, Colon, Oral cavity
- Ferments oligosaccharides in Vagina producing acid for Low pH
- Dental carries by fermenting carbs producing acids like lactic A, deminiralization
17
Q
Lactobacillus Morphology
A
- Gram Positive
- Rods
- Non-spore forming
- Anaerobic
18
Q
Lactobacillus Cultivation
A
- Tomato-juice agar
- Rogosa Medium
- Big, white, round colonies with smooth edges
19
Q
Lactobacillus Diseases
A
- Dysbiosis: Bacterial Vaginosis, Cytolytic Vaginosis
- Immunocomp: Sepsis, Endocarditis (very rare)
20
Q
Lactobacillus Treatment
A
- Bacterial Vaginosis: Metronidazole, Clindamycin
- Cytolytic Vaginosis: Sodium Bicarbonate douche, Suppository
21
Q
Lactobacillus Microbiological Diagnosis
A
- Vaginal Smear microscopy
- Clue cells: Vaginal epithelial cells with clustered bacteria show vaginosis
22
Q
Bifidobacterium General Info
A
- Normal microbial flora of Humans & Animals
- Most dominant in Colon of Infants
- Adults in Oral & Gut microbiota
- Help digestion
- Used in Probiotics
23
Q
Bifidobacterium Microbiota
A
- Oral: B. Dentium & Denticolens
- Colon: B. Adolescentis & Longum
24
Q
Bifidobacterium Morphology
A
- Gram Positive
- Rods
- Non-spore forming
- Anaerobic
25
Bifidobacterium Diseases
Non-pathogenic, if found then just contamination
26
Bifidobacterium & Infants
Microbiota produces Vitamin K produced by microbiota like Bifidobacterium
Kids need it for blood clotting or else Hemorrhagic disease of newborn
Administered after birth as injection
27
Probiotics
- Primarily Gram Positive Bacteria like Bifidobacterium & Lactobacillus, also yeast like Saccharomyces
- To improve/restore colon flora after antibiotics especially
28
Prebiotics
- Non-digestible materials for humans mainly oligosaccharides for growth of Probiotics
- Feed our bacteria
- Support our normal microbiota
