Vibrio, Plesiomonas, Aeromonas

Question 1

Vibrio Cholerae Info

Answer 1

• Not member of Enetobacteriaceae
• Fresh water & salt water
• Acid-Labile
• Like alkaline conditions
• Feco-Oral transmission

Question 2

Cholera Morphology

Answer 2

• Gram Negative Rod (comma-shaped)
• Fimbriae
• Facultative Anaerobic
• Oxidase Positive

Question 3

Cholera Cultivation

Answer 3

TCBS Agar = Thiosulfate-Citrate-Bile-Sucrose Agar
- V. Cholerae: Yellow colony
- V. Parahemolyticus: Green colony

Question 4

Cholera Virulence factors

Answer 4

Cholera toxin acts on Gs, increasing cAMP, lots of secretion in intestines and water loss

Question 5

Cholera species

Answer 5

O1, O139, El-Tor serotypes most important
- O1 divided into Ogawa, Inaba, Hokojima serotypes

Question 6

Cholera Disease

Answer 6

• Rice-water Diarrhea
• 20L of water may be lost per day
• Can lead to hypovolemic shock
• Low grade fever, vomiting

Question 7

Cholera Treatment

Answer 7

• Most important is rehydration orally or IV
• Doxycycline in severe cases

Question 8

Non-Cholera Vibrios

Answer 8

• V. Parahemolyticus (leading diarrhea in Japan)
• V. Vulnificus

Question 9

Non-Cholera Vibrios info

Answer 9

• Gram Negative curved Rods
• Halophil Vibrios (salt)
• Spread in seawater, seafood (90% oysters)

Question 10

Non-Cholera Vibrios Diseases

Answer 10

• Wound Infections (from sea water)
• Gastroenteritis (from seafood)
  Treated using Doxycycline

Question 11

Plesiomonas Shigelloides

Answer 11

• Gram Negative Rods
• Ubiquitous in water (fresh/salt)
• Oxidase Positive
• Motile
• Gastroenteritis, Sepsis
  Can be self-limiting = Carbapenem

Question 12

Aeromonas Hydrophila

Answer 12

• Gram Negative Rods
• Ubiquitous in water (fresh/salt)
• Facultative Anaerobe
• Gastroenteritis bloody & purulent, Wound infection, Pneumonia (drowning), Nosocomial
  Can be antibiotic resistant = Ciprofloxacin