Ch 2 - Microscopy Flashcards Preview

Microbiology - Biol3880 > Ch 2 - Microscopy > Flashcards

Flashcards in Ch 2 - Microscopy Deck (38)
1

Microscopy that allows for the visualization of live samples

Phase-contrast

2

microscopy image is dark cells on a light background

phase-contrast

3

improves contrast of a sample without the use of dye

phase-contrast

4

microscopy image appears light on a dark background

dark-field microscopy

5

Light reaching the lens has been scattered by specimen

dark-field microscopy

6

stylus measures weak repulsive forces between it and the specimen

atomic force microscopy

7

Uses a computerized microscope coupled with a laser source to generate a three-dimensional image

Confocal Scanning Laser Microscopy

8

Computer can focus the laser on single layers of the specimen

Confocal Scanning Laser Microscopy

9

Specimen is coated with a thin film of heavy metal

scanning electron microscope

10

very large specimens can be observed

scanning electron microscope

11

Prokaryotic/eukaryotic cells generally have a single, circular DNA molecule called a chromosome

prokaryotic

12

Prokaryotic/ Eukaryotic DNA is linear

eukaryotic

13

Typically two copies of each chromosome: prokaryotic/eukaryotic

eukaryotic

14

may have small amounts of extra-chromosomal DNA called plasmids : prokaryotic/eukaryotic

prokaryotic

15

During cell division, nucleus divides by mitosis
: prokaryotic/eukaryotic

eukaryotic

16

Evolutionary relationships between organisms

phylogeny

17

Obtain their energy from the oxidation of organic molecules

chemoorganotrophs

18

Obtain their energy from the oxidation of inorganic molecules

chemolithotrophs

19

use oxygen to obtain energy

aerobes

20

obtain energy in the absence of oxygen

anaerobes

21

Contain pigments that allow them to use light as an energy source

phototrophs

22

this photosynthesis produces oxygen

oxygenic

23

this photosynthesis does not produce oxygen

anoxygenic

24

Use carbon dioxide as their carbon source

autotrophs

25

Require one or more organic molecules for their carbon source

heterotrophs

26

All known pathogenic prokaryotes are

bacteria

27

______make up the largest phylum of Bacteria

proteobacteria

28

are proteobacteria gram (-) or Gram (+)?

Gram (-)

29

are cyanobacteria gram (-) or Gram (+)?

Gram (+)

30

what are the two phyla in the Archae domain?

Euryarchaeota , Crenarchaeota

31

degrade organic matter anaerobically, produce methane

methanogens

32

require high salt concentrations for metabolism and reproduction

extreme halophiles

33

grow in moderately high temperatures and low-pH environments

thermoacidophiles

34

Vast majority of cultured Crenarchaeota

hyperthermophiles

35

algae, fungi, protozoa, and slime molds: eukaryotic or prokaryotic

eukaryotic

36

do algae and fungi have cell walls?

yes

37

do protozoa and slime molds have cell walls?

no

38

microbes with mutualistic relationship between two groups of protists

lichens