Flashcards in Ch 11 - Genetic Engineering Deck (20)
1
recognize specific DNA sequences and cut DNA at those sites
restriction enzymes
2
Protect prokaryotes from hostile foreign DNA (e.g., viral genomes)
restriction enzymes
3
protect cell’s DNA for restriction enzymes
modification enzymes
4
method used to separate DNA molecules based on size
gel electrophoresis
5
base pairing of single strands of DNA or RNA from two different sources to give a hybrid double helix
nucleic acid hybridization
6
a hybridization procedure where DNA is in the gel and probe is RNA or DNA
southern blot
7
a hybridization procedure where RNA is in the gel and probe is RNA or DNA
northern blot
8
isolation and incorporation of a piece of DNA into a vector so it can be replicated and manipulated
molecular cloning
9
What are the three main steps of gene cloning?
1) Isolation of source DNA
2) Insertion of DNA into vector
3) Introduction of cloned DNA to host
10
enzyme that joins two DNA molecules
DNA Ligase
11
performed in vitro and introduces mutations at a precise location
site-directed mutagenesis
12
cassettes are inserted into the middle of the gene
gene disruption
13
Encode proteins that are easy to detect and assay
reporter genes
14
Promoters or coding sequences of genes of interest can be swapped with those of reporter genes to elucidate gene regulation under various conditions
gene fusions
15
Vector transfer is carried out by ______ or ________
chemical transformation or electroporation
16
in blue/white screening, ______ colonies do not have vector with foreign DNA inserted
Blue colonies
17
in blue/white screening _______ have foreign DNA inserted
white colonies
18
lacZ gene is inactivated by insertion of foreign DNA
insertional inactivation
19
allow experimenter to control the expression of cloned genes
expression vectors
20