Ch 11 - Genetic Engineering Flashcards Preview

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Flashcards in Ch 11 - Genetic Engineering Deck (20)
1

recognize specific DNA sequences and cut DNA at those sites

restriction enzymes

2

Protect prokaryotes from hostile foreign DNA (e.g., viral genomes)

restriction enzymes

3

protect cell’s DNA for restriction enzymes

modification enzymes

4

method used to separate DNA molecules based on size

gel electrophoresis

5

base pairing of single strands of DNA or RNA from two different sources to give a hybrid double helix

nucleic acid hybridization

6

a hybridization procedure where DNA is in the gel and probe is RNA or DNA

southern blot

7

a hybridization procedure where RNA is in the gel and probe is RNA or DNA

northern blot

8

isolation and incorporation of a piece of DNA into a vector so it can be replicated and manipulated

molecular cloning

9

What are the three main steps of gene cloning?

1) Isolation of source DNA
2) Insertion of DNA into vector
3) Introduction of cloned DNA to host

10

enzyme that joins two DNA molecules

DNA Ligase

11

performed in vitro and introduces mutations at a precise location

site-directed mutagenesis

12

cassettes are inserted into the middle of the gene

gene disruption

13

Encode proteins that are easy to detect and assay

reporter genes

14

Promoters or coding sequences of genes of interest can be swapped with those of reporter genes to elucidate gene regulation under various conditions

gene fusions

15

Vector transfer is carried out by ______ or ________

chemical transformation or electroporation

16

in blue/white screening, ______ colonies do not have vector with foreign DNA inserted

Blue colonies

17

in blue/white screening _______ have foreign DNA inserted

white colonies

18

lacZ gene is inactivated by insertion of foreign DNA

insertional inactivation

19

allow experimenter to control the expression of cloned genes

expression vectors

20

Effective _______ _________ are used to prevent expression of other genes on the plasmid

transcription terminators