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Flashcards in DNA Synthesis Deck (14):
1

The daughter DNA strand is synthesized in which direction?

5'-->3'

2

The template strand of DNA is read in which direction during synthesis of a daughter strand?

3'-->5'

3

DNA Pol requires what in order to replicate DNA?

Free 3' --OH

4

This enzyme adds primers to DNA for replication

Primase (RNA primer)

5

Function of telemerase

Extend telomeres using reverse transcription

6

Prokaryotic DNA replication initiation

Binding of DnaA protein at point of orgin OriC, proceeds bidirectionally

7

This enzyme adds RNA primers to DNA strand in prokaryotic replication

DNA primase

8

This DNA Pol fills in the gaps left by Okazaki fragments

Pol I

9

Function of clamp proteins (prokaryotic)

Hold DNA Pol onto template strand

10

DNA Pol that has "proofreading" function

DNA Pol III

11

DNA synthesis can only occur during what time of the eukaryotic cell cycle?

S phase

12

What is the major DNA Pol in eukaryotes?

Pol delta

13

How long are Okazaki fragments in eukaryotes?

~200 bp

14

Two functions of eukaryotic topoisomerases

1. Relieve tension (Topo I)
2. Untangle DNA (Topo II)-->cancer target, only in proliferating cells