Flashcards in FA Bacterial Genetics Deck (19):
Ability to take up naked DNA (i.e. from cell lysis) from environment (also known as "competence")
transformation is a feature especially of what three bacteria?
(FA says remember with SHiN...)
transformation uses what kind of DNA?
any naked DNA
How could you keep transformation from occurring?
Adding deoxyribouclease to environment will degrade naked DNA in medium --> no transformation seen.
What contains the genes required for sex pilus and conjugation?
What do you call a bacterium without the plasmid containing the genes required for sex pills and conjugation?
What is plasmid made out of?
what happens during F+ x F-?
plasmid is replicated and transferred through pilus from F+ to F-. no chromosomal genes transferred.
What do you call it when F+ plasmid becomes incorporated into bacterial chromosomal DNA
High frequency recombination (Hfr) cell.
Replication of incorporated plasmid DNA may include
some franking chromosomal DNA
what is Hfr x F-
Tranfer of plasmid and chromosomal genes
Segment of DNA "jumps" (excision and reintegration) from one location to another (plasmid to chromosome or vice versa)
When excision occurs during transposition, what may also be incorporated and transferred to another bacterium?
some flanking chromosomal DNA
a "packaging" event in which lytic phage infects bacterium, leading to cleavage of bacterial DNA. Parts of bacterial chromosomal DNA may become packaged in viral capsid. Phage infects another bacterium, transferring those genes.
an "excision" event in which lysogenic page infects bacterium; viral DNA incorporates into bacterial chromosome. When phage DNA is excised, flanking bacterial genes may be excised with it. DNA is packaged into phage viral capsid and can infect another bacterium.
what type of phage is associated with generalized transduction?
what type of phage is associated with specialized transduction?
genes for the following 5 bacterial toxins can be encoded in a lysogenic phage and transferred via specialized transduction (ABCDE)
ShigA like toxin
Botulinum toxin (certain strains)
Erythrogenic toxin of Strep pyogenes