Flashcards in FA Special Stains and Cultures Deck (22)
Chlamydia, Borrelia, Ricketsiae, Trypanosomes, and Plasmodium can all be visualized with what special stain?
Giema staining bugs - Certain Bugs Really Try My Patience
This special stain turns glycogen & mucopolysaccharides bring pink;
PAS (periodic acid-Schiff)
PAS the sugar
What bug can be detected by PAS?
What bugs can be detected by Ziehl-Neelsen (carbon fuchsin)?
Acid-fast organisms (Nocardia, Mycobacterium)
What bug can be detected by India ink?
What bugs can be detected by silver stain
Fungi (eg Pneumocystic), Legionella, H pylori
What bug can be isolated with chocolate agar with factors V (NAD+) and X (hematin)?
What bugs can be isolated with Thayer-Martin (or VPN) media?
N gonorrhoeae, N meningitidis
(To connect Neisseria, please use your VPN client)
What are the three components of Thayer-Martin (VPN) culture?
Why is vanco included in VPN?
Inhibits gram positive organisms
Why is polymyxin included in VPN?
inhibits gram negs except neisseria
why is nystatin included in VPN?
What can be isolated with Tellurite plate or Loffler's media? (These are two things)
What can be isolated with Lowenstein-jensen agar?
What can be isolated with Bordet-Gengous (potato) agar?
What can be isolated with Eaton's agar?
What grows pink colonies on MacConkey's agar?
Why do lactose fermentors turn MacConkey pink?
Fermentation produces acid, turning colony pink
What grows on eosin-methylene blue (EMB) as colonies with green metallic sheen?
What grows on charcoal yeast extract agar buffered with cystein and iron?