FA Special Stains and Cultures Flashcards Preview

Micro > FA Special Stains and Cultures > Flashcards

Flashcards in FA Special Stains and Cultures Deck (22):
1

Chlamydia, Borrelia, Ricketsiae, Trypanosomes, and Plasmodium can all be visualized with what special stain?

Giemsa

2

Giema staining bugs - Certain Bugs Really Try My Patience

Chlamydia
Borrelia
Rickettsiae
Trypanosomes
Plasmodium

3

This special stain turns glycogen & mucopolysaccharides bring pink;

PAS (periodic acid-Schiff)
PAS the sugar

4

What bug can be detected by PAS?

Tropheryma whipplei

5

What bugs can be detected by Ziehl-Neelsen (carbon fuchsin)?

Acid-fast organisms (Nocardia, Mycobacterium)

6

What bug can be detected by India ink?

Cryptococcus

7

What bugs can be detected by silver stain

Fungi (eg Pneumocystic), Legionella, H pylori

8

What bug can be isolated with chocolate agar with factors V (NAD+) and X (hematin)?

H influenzae

9

What bugs can be isolated with Thayer-Martin (or VPN) media?

N gonorrhoeae, N meningitidis
(To connect Neisseria, please use your VPN client)

10

What are the three components of Thayer-Martin (VPN) culture?

Vancomycin
Polymyxin
Nystatin

11

Why is vanco included in VPN?

Inhibits gram positive organisms

12

Why is polymyxin included in VPN?

inhibits gram negs except neisseria

13

why is nystatin included in VPN?

inhibits fungi

14

What can be isolated with Tellurite plate or Loffler's media? (These are two things)

C diphhtheriae

15

What can be isolated with Lowenstein-jensen agar?

M tubuerculossis

16

What can be isolated with Bordet-Gengous (potato) agar?

B pertussis

17

What can be isolated with Eaton's agar?

M pneumoniae

18

What grows pink colonies on MacConkey's agar?

Lactose-fermenting enterics

19

Why do lactose fermentors turn MacConkey pink?

Fermentation produces acid, turning colony pink

20

What grows on eosin-methylene blue (EMB) as colonies with green metallic sheen?

E coli

21

What grows on charcoal yeast extract agar buffered with cystein and iron?

Legionella

22

What grow's on Sabouraud's agar?

Fungi. Sab's a fun guy!