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Flashcards in FA Gram Neg Lab Algorithm Deck (20):
1

Gram neg, oxidase +, comma-shaped (3 bugs)

Campylobacter jejuni,
Vibrio cholerae,
Helicobacter pylori

2

Gram neg, oxidase +, comma-shaped, grows at 42 degrees C

Campylobacter jejuni

3

Gram neg, oxidase +, comma-shaped, grows in alkaline media

Vibrio cholerae

4

Gram neg, oxidase +, comma-shaped, produces urease

H pyloir

5

Lactose fermenting gram - rods

Klebsiella, E coli, Enterobacter, Cirbobacter, Serratia

6

Fast lactose fermentors (gram - rods)

Klebsiella, E coli, Enterobacter

7

Slow lactose fermentors (gram - rods)

Citrobacter, Serrati

8

Gram - rods, lactose nonfermentor, oxidase NEG

Shigella,
Salmonella
Proteus
Yersinia

9

Gram - rods, lactose nonfermentor, oxidase POS

Pseudomonas

10

Lactose fermenting enteric bacteria grow what color colonies on MacConkey agar?

PINK!

11

what bugs are lactose fermentors? test with maConKEE'S agar

citrobacter, klebsiella, e coli, enterobacter, serratia

12

How does e coli ferment lactose?

produces beta-galactosidase, which breaks down lactose into glucose and galactose

13

lactose fermentors grow as purple/black colonies on what kind of agar?

EMB

14

On EMB, what do e coli colonies look like?

purple colonies with a green sheen

15

Why are gram negs resistant to penicillin G and vancomycin?

the gram neg outer membrane lair inhibits entry of penicillin G and vanco (but may allow ampicillin or amoxicillin)

16

Gram neg coccoid rods

H influenzae,
Pasteurella
Brucella
Bordetella pertussis

17

Gram neg coccoid rod plus factor V and X requirement

H influenzae

18

gram neg coccoid plus animal bite

Pasteurella

19

Gram neg diplococci

N meningitidis or N gonorrhoeae

20

how do tell neisseria apart?

maltose (meningitidis ferments; gonorrhoeae does not)