LIPIDS AND LIPOPROTEINS part 3 Flashcards

(39 cards)

1
Q

What are the interferences of Enzymatic method?

A

Elevated levels of ascorbic acid; hemoglobin and bilirubin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What value causes the bilirubin to decrease cholesterol?

A

5 mg/dL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

If Bilirubin exceeds 5mg/dL it decreases cholesterol by how many percent?

A

5-15%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What enzyme acted on cholesterol ester and water to form cholesterol and fatty acid?

A

Cholesterol esterase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What enzyme acted on Cholesterol and oxygen to produce Cholest-4-en-one and hydrogen peroxide?

A

Cholesterol Oxidase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What enzyme acted on hydrogen peroxide and 4-aminanotipyrine to produce quinoneimine dye?

A

Peroxidase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

4-anminoantipyrine is replaceable by ___________ on the third step.

A

Methanol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

In the presence of peroxidase, what happens to methanol?

A

It is oxidized into formaldehyde

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

It is used in detecting acetylaceton

A

Formaldehyde

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

In other methods, Cholest-4-en-3-one or cholesterol is measured at?

A

240nm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

The absorbance of Quinoneimine dye is measured at?

A

500nm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

4-aminoantipyrine is AKA as?

A

4-aminophenazone

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

this uses cholesterol ester, hydrolase, and cholesterol oxidase

A

Oxygen consumption

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What causes the formation of water and oxygen under oxygen consumption?

A

Hydrogen peroxide is mixed with peroxidase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

this measures the oxygen released after the decomposition of hydrogen peroxide

A

Oxygen electrode

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

It is not easily automated unlike others, it generally requires a lot of cholesterol oxidase

A

Oxygen consumption under cholesterol

17
Q

Oxygen consumption under cholesterol requires a lot of?

A

Cholesterol oxidase

18
Q

How many molecules of FA and Glycerol do TGC contain?

19
Q

The molecules of FA and Glycerol is held by what bond?

20
Q

What is the characteristic of TGC?

A

Hydrophobic and water insoluble

21
Q

main storage formof lipid

22
Q

Where is TCG found?

A

Adipose tissues

23
Q

Where is TCG from?

A

Plant sources rich in unsaturated FA

24
Q

TCG from animal sources contains?

A

Saturated FA which are solid at room temp

25
TGC does not contain?
Charged or hydrophilic groups
26
What compactly store long carbon chains (FA) for energy that can be used during fastong states between meals?
Bod
27
TGC serves as an?
Energy source Insulation shock absorber Integral part of cell membrane
28
The breakdown of TAG is facilitated by?
LPL (Lipoprotein Lipase) Epinephrine Cortisol
29
How many hours is the fasting requirements of TGC?
10-12hrs
30
< 150 mg/dL
Normal
31
150-199 mg/dL
Borderline high
32
200-499 mg/dL
High TAG
33
>500
Very high TAG (acute and recurrent pancreatitis)
34
What do TCG evaluates and measure?
Suspected atherosclerosis body's ability to metabolize fat
35
What value of TGC is at risk for coronary artery disease?
Less than or equal to 200
36
What is the most important lipids in the management of coronary artery disease?
TCG and cholesterol
37
What causes the decrease of TAG level by 50%
Postural changes
38
What are the interferences in the methods for TGC?
Ascorbic acid Bilirubin Hemolysis
39
TAG level increases at _______ mg/dL/year between 45 to 65 years. old
2