Proteins part 1

Question 1

Proteins contains how many percent of cell’s dry weight?

Answer 1

50-70%

Question 2

Most abundant macromolecules in the body

Answer 2

Proteins

Question 3

Proteins are expressed in?

Answer 3

g/dL

Question 4

What element differentiates protein from other macromolecules.

Answer 4

Nitrogen

Question 5

Proteins are synthesize in the?

Answer 5

Liver

Question 6

These are produced in the plasma cells

Answer 6

Immunoglobulins

Question 7

positively or negatively charged.

Answer 7

Amphoteric

Question 8

TRUE OR FALSE:
Proteins are water soluble and amphoteric?

Answer 8

TRUE

Question 9

What is the charge of CHON in Alkaline pH?

Answer 9

Negative

Question 10

What is the charge of CHON in Acidic pH?

Answer 10

Positive

Question 11

What type of bond holds the AA together?

Answer 11

Peptide bonds / Amide linkages

Question 12

Give 3 essential AA

Answer 12

Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan and Valine

Question 13

Refers to the identity and specific order of amino acid residues in the polypeptide chain (amino acid sequence)

Answer 13

Primary structure

Question 14

Determines the overall shape of the protein

Answer 14

Primary structure

Question 15

Refers to the sequence of AA in a peptide or protein

Answer 15

Primary structure

Question 16

Conformation of the segments of polypeptide chain.

Answer 16

Secondary structure

Question 17

What are the sheets that composes the secondary structure?

Answer 17

Alpha-helix and beta-pleated sheet

Question 18

What type of bond maintains the secondary structure?

Answer 18

Hydrogen bond

Question 19

Intramolecular folding of the polypeptide chain into a compact 3-dimensional structure.

Answer 19

Tertiary structure

Question 20

This refers to the denaturation of proteins that occurs when temperature change or in the presence of organic solvents or reagents that may change the tertiary structure.

Answer 20

Unfolding

Question 21

What type of bond maintains tertiary structure?

Answer 21

Covalent Disulfied bond

Question 22

Association of several polypeptide subunits into a larger “oligomeric” aggregate unit.

Answer 22

Quaternary structure

Question 23

Incorporation of 2 or more polypeptide chains or subunits into a larger unit

Answer 23

Quaternary structure

Question 24

What are the examples of Quaternary structures?

Answer 24

Creatine kinase, Lactate dehydrogenase, and Hemoglobin

Question 25

How many subunits does Hemoglobin has?

Answer 25

4

Question 26

Proteins can be differentiated based on?

Answer 26

A. Differential solubility B. Molecular size C. Molecular mass D. Electrical charge E. SURFACE ADSORPTION

Question 27

– CHON are affected by pH, ionic strength, temperature and dielectric constant.

Answer 27

Differential solubility

Question 28

pH where proteins have no net charge

Answer 28

Isoelectric point

Question 29

ions that has 2 different charges but net charge on the molecule is ZERO.

Answer 29

Zwitterions

Question 30

Accomplished through ultracentrifugation or dialysis

Answer 30

Molecular size

Question 31

Can be accomplished through mass spectrometry

Answer 31

Molecular mass

Question 32

Can be accomplished using ion exchange chromatography and electrophoresis.

Answer 32

Electrical charge

Question 33

Can be done using chromatography

Answer 33

Surface adsorption

Question 34

Between serum and plasma, which one contains Fibrinogen?

Answer 34

Plasma

Question 35

Due to the absence of Fibrinogen in serum, the total content decreases into how many percent?

Answer 35

4%